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The official blog of Cell Signaling Technology, where we discuss what to expect from your time at the bench, share tips, tricks, and information.

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Why use viability dyes in flow cytometry experiments?

Viability dyes are widely used when counting and passaging cells, assessing chemical toxicity, and p...
Rob MacDonald, PhD Nov 17, 2021

Tools for Modulating Cells

Cell behavior is a tightly regulated, finely tuned process where various different signals work toge...
Andrea Tu, PhD Oct 13, 2021

Assay Kits and Tools for Monitoring Cell State

Have you been wondering how to monitor a cell state or cellular response? From a basic research stan...
Andrea Tu, PhD Oct 6, 2021

How S6 Ribosomal Protein Rabbit Antibody Can Help with Your IF Staining

One antibody is used in our lab so often that we each keep our own bulk stock! This antibody is S6 R...

A Tale of Two Stains: Ponceau S vs Coomassie Blue

You've just finished running your lysate sample by SDS-PAGE, so you're pretty confident your protein...

Western Blotting: Best Practices for Cell Lysate Preparation

The preparation of a cell lysate is crucial to the success of many assays, including techniques that...
Michelle Graham Sep 2, 2020

Cell Process: How is cytotoxicity assessed?

Changes in cellular health in response to exogenous stimuli can provide keen insight into the biolog...
Tamar Aprahamian, PhD Jul 8, 2020

Seven Assays to Detect Apoptosis

Apoptosis is a highly regulated form of programmed cell death that occurs in multicellular organisms...
Tamar Aprahamian, PhD Jul 1, 2020

Control Your Western Blot: Loading Control Expression in Mouse Tissues

When it comes to mouse tissue samples, not all western blot (WB) loading control proteins are expres...
Supriya Singh, PhD Jun 24, 2020

Antibody Selection for Epitope Tagging: Insights from Myc-Tag Performance

Biological research is about expanding knowledge, so it's not uncommon for scientists to study prote...
Tamar Aprahamian, PhD May 20, 2020

10 Must-Have Markers for Senescence Research

Understanding when and why cell cycle arrest occurs is critical to many fields of research, includin...
Tamar Aprahamian, PhD May 13, 2020

How is cell proliferation quantified?

The production of new cells through cellular proliferation impacts the development, growth, and main...
Tamar Aprahamian, PhD Apr 22, 2020

What is cell viability and how can it be measured?

The health of cells in culture is critical to the success of your experiments. Have you ever been ex...
Tamar Aprahamian, PhD Apr 15, 2020

Dual Staining Mouse CD8 and FoxP3 with Confidence

For decades, immunohistochemistry (IHC) has been a powerful technique for the investigation and visu...
Tamar Aprahamian, PhD Sep 25, 2019

How to Prepare a ChIP-seq DNA Library

Chromatin immunoprecipitation sequencing (ChIP-seq) is a flexible and powerful technique used by res...
Tamar Aprahamian, PhD Sep 18, 2019

Enzymatic vs Sonication: Optimizing Chromatin Prep for Successful ChIP

Part one of this series described the importance of including proper controls in your ChIP protocols...
Carolyn P Jun 26, 2019

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