CST BLOG: Lab Expectations

The official blog of Cell Signaling Technology (CST), where we discuss what to expect from your time at the bench, share tips, tricks, and information.

Lab Expectations listing page

Read More →

4 Situations Where Flow Cytometry Might Be More Useful Than Western Blotting

Western blotting is used to detect proteins and analyze their expression using antibodies, but it do...
Chris Sumner Dec 4, 2019

Critical Considerations for Optimizing Imaging Mass Cytometry

Understanding the microenvironment of disease is critical for the success of targeted therapies. For...
Chris Sumner Nov 20, 2019

Preparing a ChIP-seq DNA Library. Where do I start?

Chromatin immunoprecipitation sequencing (ChIP-seq) is a flexible and powerful technique used by res...
Tamar Aprahamian, PhD Sep 18, 2019

Visualize and Understand Protein Co-expression during EMT

Careful planning and the fine tuning of experimental protocols are key to ensuring clear, interpreta...
Tamar Aprahamian, PhD Aug 7, 2019

How to Choose Enzymatic or Sonication Protocol for ChIP

Researchers use chromatin immunoprecipitation, or ChIP, to identify and characterize protein-DNA int...
Chris Sumner Jun 26, 2019

Video: Can you trust your immunohistochemistry results?

Immunohistochemistry, or IHC, remains the simplest method for detecting biomarker expression while m...
Chris Sumner Jun 19, 2019

Need cell type and function? Use flow cytometry!

What is flow cytometry and how is it used? Flow cytometry enables you to save time and analyze many ...
Tamar Aprahamian, PhD May 8, 2019

General Tips for Successful Cell Culture

Culturing cells in the lab? Following these tips will add to your success and help you avoid wasted ...
Dan D Apr 24, 2019

STR Profiling: What's Growing in Your Incubator?

Cell lines are a crucial part of life science research and development. But did you know an estimate...
Hayley R Mar 6, 2019

When should I use flow cytometry for signaling instead of western blot?

Flow cytometry enables quantitative analysis of protein expression, signaling states, and physical c...
Chris Sumner Jan 9, 2019

Mycoplasma Contamination: Steps for Prevention, Testing, and Treatment

Any form of cell culture contamination can ruin your day and destroy your hard work, but mycoplasma ...
Michelle Graham Dec 5, 2018

Tech Tips Video: How much antibody should I use for ChIP?

It's time to check out another video from the CST Tech Tips playlist! In this edition of Tech Tips, ...
Kenneth Buck, PhD Nov 28, 2018

Myth-busting for ChIP/ChIP-seq: Using Mono vs Polyclonal Antibodies

Researchers who run a lot of chromatin immunoprecipitation "ChIP" assays—maybe even your advisor—mig...
Kenneth Buck, PhD Sep 26, 2018

Milk or BSA? How to Choose a Blocking Protein for Western Blot [Video]

So you've set the timer to five minutes for the first of three TBST washes for your western blot mem...
Kenneth Buck, PhD Sep 5, 2018

Mastering Phospho-Flow: Immune Cell Activation Webinar

To characterize activation of immune cells, measurement of intracellular signaling using phospho-spe...
Chris Sumner Jul 11, 2018

Are you ready to transfer your protein? Wet vs Semi-dry Transfer

You are all amped up to run a western blot to identify “your favorite protein.” The lysates have bee...
Neha G Apr 18, 2018

Keep ChIP’ing Away

So your experiments and data are funneling you down an inescapable path. You need to show direct gen...
Curtis Desilets Jan 31, 2018

Western Blot Normalization: Am I setting up my loading controls correctly?

You’re gathering data from all your experiments and preparing to present to your advisor and thesis ...
Kenneth Buck, PhD Nov 1, 2017

      Categories

      CST Newsletter

      Popular Posts

      Recent Posts
      landscape-blue-blog-gradient