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The official blog of Cell Signaling Technology, where we discuss what to expect from your time at the bench, share tips, tricks, and information.

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10 Must-Have Markers for Senescence Research

Understanding when and why cell cycle arrest occurs is critical to many fields of research, includin...
Tamar Aprahamian, PhD May 13, 2020

What is cell viability and how can it be measured?

The health of cells in culture is critical to the success of your experiments. Have you ever been ex...
Tamar Aprahamian, PhD Apr 15, 2020

Dual Staining Mouse CD8 and FoxP3 with Confidence

For decades, immunohistochemistry (IHC) has been a powerful technique for the investigation and visu...
Tamar Aprahamian, PhD Sep 25, 2019

How to Prepare a ChIP-seq DNA Library

Chromatin immunoprecipitation sequencing (ChIP-seq) is a flexible and powerful technique used by res...
Tamar Aprahamian, PhD Sep 18, 2019

Enzymatic vs Sonication: Optimizing Chromatin Prep for Successful ChIP

Part one of this series described the importance of including proper controls in your ChIP protocols...
Carolyn P Jun 26, 2019

Tissue Clearing Imaging Methods for Whole, Intact Brain Imaging

For generations of neuroscientists, using immunohistochemistry to study the brain in its anatomical ...
Richard Cho, PhD Jun 5, 2019

Need cell type and function? Use flow cytometry

What is flow cytometry and how is it used? Flow cytometry enables you to save time and analyze many ...
Tamar Aprahamian, PhD May 8, 2019

General Tips for Successful Cell Culture

Culturing cells in the lab? Following these tips will add to your success and help you avoid wasted ...

STR Profiling: What's Growing in Your Incubator?

Cell lines are a crucial part of life science research and development. But did you know an estimate...

Can I accelerate my ELISA results?

Running an ELISA can be a pain. Identifying pairs for an ELISA is a tedious business, and that’s bef...
Chris Sumner Jan 16, 2019

When should I use flow cytometry instead of western blot?

Western blotting is used to detect proteins and analyze their expression using antibodies, but it do...

Mycoplasma Cell Contamination: Tips for Prevention, Testing & Treatment

Any form of cell culture contamination can ruin your day and destroy your hard work, but mycoplasma ...
Michelle Graham Dec 5, 2018

Demystifying Antibody Panel Design for Multiplex IHC

In recent years, immune checkpoints in the tumor microenvironment have been under intense study. If ...
Jen Z Oct 10, 2018

ChIP & ChIP-seq: Should I Use Monoclonal or Polyclonal Antibodies?

Researchers who run a lot of chromatin immunoprecipitation ChIP assays—maybe even your advisor—might...
Kenneth Buck, PhD Sep 26, 2018

Milk or BSA? How to Choose a Blocking Protein for Western Blot [Video]

So you've set the timer to five minutes for the first of three TBST washes for your western blot mem...
Kenneth Buck, PhD Sep 5, 2018

Are you ready to transfer your protein? Wet vs Semi-dry Transfer

You are all amped up to run a western blot to identify “your favorite protein.” The lysates have bee...

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