CST BLOG: Lab Expectations

The official blog of Cell Signaling Technology (CST), where we discuss what to expect from your time at the bench, share tips, tricks, and information.

Lab Expectations listing page

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How S6 Ribosomal Protein (5G10) Rabbit Antibody Can Help You With Your IF Staining

One antibody is used in our lab so often that we each keep our own bulk stock! This antibody is S6 R...

A Tale of Two Stains: Ponceau S vs Coomassie Blue

You've just finished running your lysate sample by SDS-PAGE, so you're pretty confident your protein...

Western Blotting: Best Practices for Cell Lysate Preparation

The preparation of a cell lysate is crucial to the success of many assays, including techniques that...
Michelle Graham Sep 2, 2020

Pros and Cons of Different Multiplexing Techniques for IHC and IF

Multiplex immunohistochemistry (mIHC) and multiplex immunofluorescence (mIF) are widely used to iden...

Control Your Western Blot: Loading Control Expression in Mouse Tissues

When it comes to mouse tissue samples, not all western blot (WB) loading control proteins are expres...
Supriya Singh, PhD Jun 24, 2020

How to Prepare a ChIP-seq DNA Library

Chromatin immunoprecipitation sequencing (ChIP-seq) is a flexible and powerful technique used by res...
Tamar Aprahamian, PhD Sep 18, 2019

Enzymatic vs Sonication: Optimizing Chromatin Prep for Successful ChIP

Part one of this series described the importance of including proper controls in your ChIP protocols...
Carolyn P Jun 26, 2019

If I Could Only See What’s Going on Inside Your Brain...

For generations of neuroscientists, using immunohistochemistry to study the brain in its anatomical ...
Richard Cho, PhD Jun 5, 2019

Need cell type and function? Use flow cytometry

What is flow cytometry and how is it used? Flow cytometry enables you to save time and analyze many ...
Tamar Aprahamian, PhD May 8, 2019

General Tips for Successful Cell Culture

Culturing cells in the lab? Following these tips will add to your success and help you avoid wasted ...

STR Profiling: What's Growing in Your Incubator?

Cell lines are a crucial part of life science research and development. But did you know an estimate...

Can I accelerate my ELISA results?

Running an ELISA can be a pain. Identifying pairs for an ELISA is a tedious business, and that’s bef...
Chris Sumner Jan 16, 2019

When should I use flow cytometry instead of western blot?

Western blotting is used to detect proteins and analyze their expression using antibodies, but it do...

Mycoplasma Cell Contamination: Tips for Prevention, Testing & Treatment

Any form of cell culture contamination can ruin your day and destroy your hard work, but mycoplasma ...
Michelle Graham Dec 5, 2018

Demystifying Antibody Panel Design for Multiplex IHC

In recent years, immune checkpoints in the tumor microenvironment have been under intense study. If ...
Jen Z Oct 10, 2018

ChIP & ChIP-seq: Should I Use Monoclonal or Polyclonal Antibodies?

Researchers who run a lot of chromatin immunoprecipitation ChIP assays—maybe even your advisor—might...
Kenneth Buck, PhD Sep 26, 2018

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