CST BLOG: Lab Expectations

The official blog of Cell Signaling Technology (CST), where we discuss what to expect from your time at the bench, share tips, tricks, and information.

Lab Expectations listing page

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Assay Kits and Tools for Monitoring Cell State

Have you been wondering how to monitor a cell state or cellular response? From a basic research stan...
Andrea Tu, PhD Oct 6, 2021

How S6 Ribosomal Protein (5G10) Rabbit Antibody Can Help You With Your IF Staining

One antibody is used in our lab so often that we each keep our own bulk stock! This antibody is S6 R...

A Tale of Two Stains: Ponceau S vs Coomassie Blue

You've just finished running your lysate sample by SDS-PAGE, so you're pretty confident your protein...

Western Blotting: Best Practices for Cell Lysate Preparation

The preparation of a cell lysate is crucial to the success of many assays, including techniques that...
Michelle Graham Sep 2, 2020

Pros and Cons of Different Multiplexing Techniques for IHC and IF

Multiplex immunohistochemistry (mIHC) and multiplex immunofluorescence (mIF) are widely used to iden...

Control Your Western Blot: Loading Control Expression in Mouse Tissues

When it comes to mouse tissue samples, not all western blot (WB) loading control proteins are expres...
Supriya Singh, PhD Jun 24, 2020

Critical Considerations for Optimizing Imaging Mass Cytometry

Understanding the microenvironment of disease is critical for the success of targeted therapies. For...
Chris Sumner Nov 20, 2019

How to Prepare a ChIP-seq DNA Library

Chromatin immunoprecipitation sequencing (ChIP-seq) is a flexible and powerful technique used by res...
Tamar Aprahamian, PhD Sep 18, 2019

How to Choose Enzymatic or Sonication Protocol for ChIP

Researchers use chromatin immunoprecipitation, or ChIP, to identify and characterize protein-DNA int...
Chris Sumner Jun 26, 2019

Video: Can you trust your immunohistochemistry results?

Immunohistochemistry, or IHC, remains the simplest method for detecting biomarker expression while m...
Chris Sumner Jun 19, 2019

If I Could Only See What’s Going on Inside Your Brain...

For generations of neuroscientists, using immunohistochemistry to study the brain in its anatomical ...
Richard Cho, PhD Jun 5, 2019

Need cell type and function? Use flow cytometry!

What is flow cytometry and how is it used? Flow cytometry enables you to save time and analyze many ...
Tamar Aprahamian, PhD May 8, 2019

General Tips for Successful Cell Culture

Culturing cells in the lab? Following these tips will add to your success and help you avoid wasted ...
Dan D Apr 24, 2019

STR Profiling: What's Growing in Your Incubator?

Cell lines are a crucial part of life science research and development. But did you know an estimate...

Can I accelerate my ELISA results?

Running an ELISA can be a pain. Identifying pairs for an ELISA is a tedious business, and that’s bef...
Chris Sumner Jan 16, 2019

When should I use flow cytometry instead of western blot?

Western blotting is used to detect proteins and analyze their expression using antibodies, but it do...

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