CST BLOG: Lab Expectations

The official blog of Cell Signaling Technology (CST), where we discuss what to expect from your time at the bench, share tips, tricks, and information.

Lab Expectations listing page

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A Tale of Two Stains: Ponceau S vs Coomassie Blue

You've just finished running your lysate sample by SDS-PAGE, so you're pretty confident your protein...

Recommended Best Practices for Lysate Handling

The preparation of a cell lysate is crucial to the success of many assays, including techniques that...
Michelle Graham Sep 2, 2020

Control Your Western Blot: Loading Control Expression in Mouse Tissues

When it comes to mouse tissue samples, not all western blot loading control proteins are expressed e...
Supriya Singh, PhD Jun 24, 2020

4 Situations Where Flow Cytometry Might Be More Useful Than Western Blotting

Western blotting is used to detect proteins and analyze their expression using antibodies, but it do...
Chris Sumner Dec 4, 2019

Critical Considerations for Optimizing Imaging Mass Cytometry

Understanding the microenvironment of disease is critical for the success of targeted therapies. For...
Chris Sumner Nov 20, 2019

Preparing a ChIP-seq DNA Library. Where do I start?

Chromatin immunoprecipitation sequencing (ChIP-seq) is a flexible and powerful technique used by res...
Tamar Aprahamian, PhD Sep 18, 2019

Visualize and Understand Protein Co-expression during EMT

Careful planning and the fine tuning of experimental protocols are key to ensuring clear, interpreta...
Tamar Aprahamian, PhD Aug 7, 2019

How to Choose Enzymatic or Sonication Protocol for ChIP

Researchers use chromatin immunoprecipitation, or ChIP, to identify and characterize protein-DNA int...
Chris Sumner Jun 26, 2019

Video: Can you trust your immunohistochemistry results?

Immunohistochemistry, or IHC, remains the simplest method for detecting biomarker expression while m...
Chris Sumner Jun 19, 2019

If I Could Only See What’s Going on Inside Your Brain...

For generations of neuroscientists, using immunohistochemistry to study the brain in its anatomical ...
Richard Cho, PhD Jun 5, 2019

Need cell type and function? Use flow cytometry!

What is flow cytometry and how is it used? Flow cytometry enables you to save time and analyze many ...
Tamar Aprahamian, PhD May 8, 2019

General Tips for Successful Cell Culture

Culturing cells in the lab? Following these tips will add to your success and help you avoid wasted ...
Dan D Apr 24, 2019

STR Profiling: What's Growing in Your Incubator?

Cell lines are a crucial part of life science research and development. But did you know an estimate...
Hayley R Mar 6, 2019

When should I use flow cytometry for signaling instead of western blot?

Flow cytometry enables quantitative analysis of protein expression, signaling states, and physical c...
Chris Sumner Jan 9, 2019

Mycoplasma Contamination: Steps for Prevention, Testing, and Treatment

Any form of cell culture contamination can ruin your day and destroy your hard work, but mycoplasma ...
Michelle Graham Dec 5, 2018

Tech Tips Video: How much antibody should I use for ChIP?

It's time to check out another video from the CST Tech Tips playlist! In this edition of Tech Tips, ...
Kenneth Buck, PhD Nov 28, 2018

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