CST BLOG: Lab Expectations

The official blog of Cell Signaling Technology (CST), where we discuss what to expect from your time at the bench, share tips, tricks, and information.

Lab Expectations listing page

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Cell Preparation Tips for Accurate Flow Cytometry Data

Generating accurate flow cytometry data requires more than just specific and sensitive antibodies va...
Andrea Tu, PhD Nov 20, 2024

Faster Immuno-Oncology Research with Rapid T Cell Activation & Expansion

The success of immuno-oncology therapeutics has opened the door to novel cancer elimination strategi...
Andrea Tu, PhD Sep 20, 2023

Faster CAR-T Cell Detection with Flow Cytometry-Validated Anti-CAR Linker Antibodies

CST® anti-CAR linker antibodies won The Innovation Award from CiteAb in 2023. Read on to learn why! ...
Amrik Singh, PhD Nov 8, 2022

Which Fluorophores Can I Combine in My Flow Cytometry Experiment?

Flow cytometry offers the exciting ability to detect and quantify multiple readouts per cell, enabli...
Rob MacDonald, PhD Apr 13, 2022

Antibody Staining Guide for Flow Cytometry

Getting started with flow cytometry or beginning to branch out from working solely with live cells t...
Rob MacDonald, PhD Mar 23, 2022

Why use viability dyes in flow cytometry experiments?

Viability dyes are widely used when counting and passaging cells, assessing chemical toxicity, and p...
Rob MacDonald, PhD Nov 17, 2021

Immunology: Immunophenotyping Basics

Immunophenotyping is a technique that uses antibodies to allow the identification and quantification...
Chris Sumner Jun 30, 2021

4 Situations Where Flow Cytometry Might Be More Useful Than Western Blotting

Western blotting is used to detect proteins and analyze their expression using antibodies, but it do...
Chris Sumner Dec 4, 2019

Need cell type and function? Use flow cytometry!

What is flow cytometry and how is it used? Flow cytometry enables you to save time and analyze many ...
Tamar Aprahamian, PhD May 8, 2019

When should I use flow cytometry for signaling instead of western blot?

Flow cytometry enables quantitative analysis of protein expression, signaling states, and physical c...
Chris Sumner Jan 9, 2019

Mastering Phospho-Flow: Immune Cell Activation Webinar

To characterize activation of immune cells, measurement of intracellular signaling using phospho-spe...
Chris Sumner Jul 11, 2018

Webinar | Highly Multiplexed Single Cell Analysis of Tumor Heterogeneity through Time and Space by Mass Cytometry

The study of the tumor ecosystem and its cell-to-cell communications is essential to enable an under...
Chris Sumner Jun 21, 2017

Intracellular Flow Cytometry in Action

Traditionally, flow cytometry has been used to identify distinct cell types within a heterogeneous p...
Liana G Aug 12, 2015

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