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The official blog of Cell Signaling Technology, where we discuss what to expect from your time at the bench, share tips, tricks, and information.

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What support reagents do I need for accurate flow cytometry data?

Generating accurate flow cytometry data requires more than just specific and sensitive antibodies va...
Andrea Tu, PhD Nov 6, 2024

ChIP, CUT&RUN & CUT&Tag: Support Reagents for Successful Chromatin Profiling

If you’ve performed CUT&RUN, CUT&Tag, or ChIP/ChIP-seq experiments, you know how important i...
Andrea Tu, PhD May 8, 2024

Too Low to Detect? Your CUT&Tag Library May Still Be Worth Sequencing

You load your Bioanalyzer, hold your breath, and watch the trace appear. Instead of a clean peak, yo...
Andrea Tu, PhD Feb 5, 2024

How to Control Your Apoptosis, Autophagy, & Mitophagy Western Blots

Have you ever spent time treating cells, preparing cell extracts, and performing a western blot only...
Whitney Palmer Dec 4, 2023

How to Accurately Count Cells Manually or with an Automated Cell Counter

There are several situations in the lab where you’ll need to know the number of cells in a given vol...
Kenneth Buck, PhD May 17, 2023

How do I choose a loading control for my western blot?

Think about the last time you generated western blot data. Did you find the predicted differences in...
Supriya Singh, PhD Feb 1, 2023

Trim-Away: Rapid, Easy, and Specific Protein Degradation

Foundational approaches for studying protein function in living models—gene editing and RNA interfer...

Increase Your Experimental Success with CUT&RUN-Validated Antibodies

CUT&RUN delivers the power of chromatin immunoprecipitation (ChIP) or ChIP-sequencing (ChIP-seq)...
Christopher Fry, PhD Oct 26, 2022

Comparing Antibody Concentrations & Performance; CST Antibody Details You May Not Know

When purchasing antibodies from Cell Signaling Technology (CST), you may notice that we typically re...
Josh Nathan Oct 12, 2022

Which Fluorophores Can I Combine in My Flow Cytometry Experiment?

Flow cytometry offers the exciting ability to detect and quantify multiple readouts per cell, enabli...
Rob MacDonald, PhD Apr 13, 2022

Strategies for Successful Multiplex IF Experiments Using Conjugated Antibodies

One of the benefits of working with immunofluorescent (IF) analysis is the ability to compare multip...
Ginny Bain, PhD Apr 6, 2022

Flow Cytometry Antibody Staining Guide: Surface vs. Intracellular Protocols Explained

Getting started with flow cytometry or beginning to branch out from working solely with live cells t...
Rob MacDonald, PhD Mar 23, 2022

Tools for Classifying Cells and Identifying Protein Localization

When do I want to classify cells? Why do I care where a protein localizes? These are actually two di...
Andrea Tu, PhD Jan 26, 2022

Subcellular Marker Selection for Immunofluorescence

When performing immunofluorescence (IF) experiments, researchers often multiplex with several antibo...
Ginny Bain, PhD Jan 12, 2022

Analyzing PTM Activation State Using Western Blotting

Post-translational modifications (PTMs) are a critical regulatory mechanism that allows cells to tig...
Andrea Tu, PhD Dec 8, 2021

Tools to Perform Genomic Profiling

Techniques used in genomic profiling have come a long way in the past few decades. Researchers can n...
Curtis Desilets Dec 1, 2021

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