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The official blog of Cell Signaling Technology, where we discuss what to expect from your time at the bench, share tips, tricks, and information.

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Reliable Data Normalization for CUT&RUN and CUT&Tag with Whole Workflow Spike-In Controls

If you use CUT&RUN or CUT&Tag to map histone modifications, DNA methylation, transcription f...
Alexandra Foley Mar 18, 2026

ChIP, CUT&RUN & CUT&Tag: Support Reagents for Successful Chromatin Profiling

If you’ve performed CUT&RUN, CUT&Tag, or ChIP/ChIP-seq experiments, you know how important i...
Andrea Tu, PhD May 8, 2024

Too Low to Detect? Your CUT&Tag Library May Still Be Worth Sequencing

You load your Bioanalyzer, hold your breath, and watch the trace appear. Instead of a clean peak, yo...
Andrea Tu, PhD Feb 5, 2024

Increase Your Experimental Success with CUT&RUN-Validated Antibodies

CUT&RUN delivers the power of chromatin immunoprecipitation (ChIP) or ChIP-sequencing (ChIP-seq)...
Christopher Fry, PhD Oct 26, 2022

Tools to Perform Genomic Profiling

Techniques used in genomic profiling have come a long way in the past few decades. Researchers can n...
Curtis Desilets Dec 1, 2021

How to Prepare a ChIP-seq DNA Library

Chromatin immunoprecipitation sequencing (ChIP-seq) is a flexible and powerful technique used by res...
Tamar Aprahamian, PhD Sep 18, 2019

Enzymatic vs Sonication: Optimizing Chromatin Prep for Successful ChIP

Part one of this series described the importance of including proper controls in your ChIP protocols...
Carolyn P Jun 26, 2019

ChIP & ChIP-seq: Should I Use Monoclonal or Polyclonal Antibodies?

Researchers who run a lot of chromatin immunoprecipitation ChIP assays—maybe even your advisor—might...
Kenneth Buck, PhD Sep 26, 2018

From PCR to ChIP-seq: How to Analyze Your ChIP Data

Part one of this series described the importance of including proper controls in your protocols. In ...
Carolyn P Mar 11, 2015

Signal vs Noise: Choosing the Right Antibody for Successful ChIP

Part one of this series described the importance of including proper controls to your protocols. In ...
Carolyn P Mar 4, 2015

Take Control: How to Use Positive and Negative Controls for Better ChIP Results

You're a confocal maestro, playing the lasers and the filters and the gain settings like a Stradivar...
Carolyn P Feb 18, 2015

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