A Tale of Two Stains: Ponceau S vs Coomassie Blue

Posted by Caroline J and Misbah K on Dec 9, 2020 3:00:00 AM

You've just finished running your lysate sample by SDS-PAGE, so you're pretty confident your protein should have migrated at the right molecular weight and is ready to transfer to a membrane for detection by Western Blot. But what if it's not?


Topics: Companion Reagents, techniques, Tech Tips

Recommended Best Practices for Lysate Handling

Posted by Michelle G on Sep 2, 2020 3:00:00 AM

The preparation of a cell lysate is crucial to the success of many assays, including techniques that use antibodies to characterize protein expression. Ideally, throughout the whole process of sample preparation, from lysate collection to analysis, your protein of interest would be well-preserved and protected from the activities of proteases and phosphatases. Proper storage and handling of the lysate are crucial to avoiding degradation and maintaining the ability to detect your protein.


Topics: Western Blot, techniques, Tech Tips

General Tips for Successful Cell Culture

Posted by Dan D on Apr 24, 2019 3:10:00 AM

Culturing cells in the lab? Following these tips will add to your success and help you avoid wasted time or the dreaded cell contamination!


Topics: techniques, Science Education, Tech Tips

Introducing Cell Mentor

Posted by Chris S on Apr 10, 2019 3:15:00 AM

In collaboration with Cell Press, we are excited to announce a new educational resource that will make it easier for biology students and researchers to navigate their careers, get published, and strengthen their laboratory skills to enable experimental success. That new resource is called Cell Mentor™.


Topics: Reproducibility, Career Development, Science Education, Tech Tips

Tech Tips Video: How much antibody should I use for ChIP?

Posted by Ken B on Nov 28, 2018 3:15:00 AM

It's time to check out another video from the CST Tech Tips playlist! In this edition of Tech Tips, we'll tackle a common protocol question customers ask our ChIP team: how much antibody to use for chromatin immunoprecipitaion (ChIP) experiments. Adding more antibody isn't always better - watch the video to learn why. 

Can't see the embedded video above? Click the link below to play: 

How much antibody should I use in ChIP assays? | CST Tech Tips

Don't forget to subscribe to CST's YouTube channel for more videos to help your research!



Topics: Protocols, Primary Antibodies, ChIP, Tech Tips

Tech Tips Video: Milk or BSA? Choosing a blocking protein for Western Blot

Posted by Ken B on Sep 5, 2018 3:00:00 AM

So you've set the timer to five minutes for the first of three TBST washes for your western blot membrane. Now what? Sure, you could check your email or social media for the 30th time before lunch. Or you could do something informative, like check out a CST Tech Tips video! This is a new short video series featuring the same scientists who develop and validate CST antibodies, here to offer insights and protocol tips.


Topics: Protocols, Western Blot, techniques, Tech Tips

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