CST BLOG: Lab Expectations

The official blog of Cell Signaling Technology (CST), where we discuss what to expect from your time at the bench, share tips, tricks, and information.

Lab Expectations listing page

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Beyond Your Primary Antibody: What You Need for a Successful Chromatin Profiling Experiment

If you’ve performed CUT&RUN, CUT&Tag, or ChIP/ChIP-seq experiments, you know how important i...
Andrea Tu, PhD May 8, 2024

How to Control Your Apoptosis, Autophagy, and Mitophagy Western Blots

Have you ever spent time treating cells, preparing cell extracts, and performing a western blot only...
Whitney Palmer Dec 4, 2023

How to Accurately Count Cells Manually or with an Automated Cell Counter

There are several situations in the lab where you’ll need to know the number of cells in a given vol...
Kenneth Buck, PhD May 17, 2023

How do I choose a loading control for my western blot?

Think about the last time you generated western blot data. Did you find the predicted differences in...
Supriya Singh, PhD Feb 1, 2023

Which Fluorophores Can I Combine in My Flow Cytometry Experiment?

Flow cytometry offers the exciting ability to detect and quantify multiple readouts per cell, enabli...
Rob MacDonald, PhD Apr 13, 2022

Antibody Staining Guide for Flow Cytometry

Getting started with flow cytometry or beginning to branch out from working solely with live cells t...
Rob MacDonald, PhD Mar 23, 2022

Tools to Initiate Discovery

You’re at an impasse. Maybe you’re embarking on a new research project, or your current project has ...
Chris Sumner Mar 16, 2022

Tools for Classifying Cells and Identifying Protein Localization

When do I want to classify cells? Why do I care where a protein localizes? These are actually two di...
Andrea Tu, PhD Jan 26, 2022

Subcellular Marker Selection for Immunofluorescence

When performing immunofluorescence (IF), it is common to multiplex with several antibodies and/or dy...
Ginny Bain, PhD Jan 12, 2022

Analyzing PTM Activation State Using Western Blotting

Post-translational modifications (PTMs) are a critical regulatory mechanism that allows cells to tig...
Andrea Tu, PhD Dec 8, 2021

Tools to Perform Genomic Profiling

Techniques used in genomic profiling have come a long way in the past few decades. Researchers can n...
Curtis Desilets Dec 1, 2021

Tools for Screening and Quantifying Proteins

Whether your heart is devoted to cardiac research, you’re excited to investigate neurodegenerative d...
Steve M Oct 20, 2021

Tools for Modulating Cells

Cell behavior is a tightly regulated, finely tuned process where various different signals work toge...
Andrea Tu, PhD Oct 13, 2021

Tools for Monitoring Cell State

Have you been wondering how to monitor a cell state or cellular response? From a basic research stan...
Andrea Tu, PhD Oct 6, 2021

How S6 Ribosomal Protein (5G10) Rabbit mAb Can Help You With Your Immunofluorescence Staining

One antibody is used in our lab so often that we each keep our own bulk stock! This antibody is S6 R...
John B Jan 27, 2021

A Tale of Two Stains: Ponceau S vs Coomassie Blue

You've just finished running your lysate sample by SDS-PAGE, so you're pretty confident your protein...

Recommended Best Practices for Lysate Handling

The preparation of a cell lysate is crucial to the success of many assays, including techniques that...
Michelle G Sep 2, 2020

Loading Control Expression in Mouse Tissues

When it comes to mouse tissue samples, not all western blot loading control proteins are expressed e...
Supriya Singh, PhD Jun 24, 2020

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