CST BLOG: Lab Expectations

The official blog of Cell Signaling Technology (CST), where we discuss what to expect from your time at the bench, share tips, tricks, and information.

Lab Expectations listing page

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How to Accurately Count Cells Manually or with an Automated Cell Counter

There are several situations in the lab where you’ll need to know the number of cells in a given vol...
Kenneth Buck, PhD May 17, 2023

Trim-Away: Rapid, Easy, and Specific Protein Degradation

Foundational approaches for studying protein function in living models—gene editing and RNA interfer...

Strategies for Successful Multiplex IF Experiments Using Conjugated Antibodies

One of the benefits of working with immunofluorescent (IF) analysis is the ability to compare multip...
Ginny Bain, PhD Apr 6, 2022

Subcellular Marker Selection for Immunofluorescence

When performing immunofluorescence (IF), it is common to multiplex with several antibodies and/or dy...
Ginny Bain, PhD Jan 12, 2022

Multiplexing the Brain: A Galaxy of Possibilities

The average human brain contains over 100 billion cells, which is nearly the same number as there ar...
Richard Cho, PhD Dec 15, 2021

Analyzing PTM Activation State Using Western Blotting

Post-translational modifications (PTMs) are a critical regulatory mechanism that allows cells to tig...
Andrea Tu, PhD Dec 8, 2021

Tools for Monitoring Cell State

Have you been wondering how to monitor a cell state or cellular response? From a basic research stan...
Andrea Tu, PhD Oct 6, 2021

A Tale of Two Stains: Ponceau S vs Coomassie Blue

You've just finished running your lysate sample by SDS-PAGE, so you're pretty confident your protein...

Recommended Best Practices for Lysate Handling

The preparation of a cell lysate is crucial to the success of many assays, including techniques that...
Michelle G Sep 2, 2020

Pros and Cons of Different Multiplexing Techniques for mIHC and mIF

Multiplex immunohistochemistry (mIHC) and multiplex immunofluorescence (mIF) are widely used to iden...
Emma E Jul 22, 2020

Video: How to Choose Enzymatic or Sonication Protocol for ChIP

The success of your chromatin immunoprecipitation (ChIP) experiment depends on the fragmentation of ...
Kenneth Buck, PhD Nov 6, 2019

IMC: Endless Possibilities to Understand the Microenvironment of Disease

Understanding the microenvironment of disease is critical. Targeted cancer therapies may not be effe...
Kris H Oct 23, 2019

General Tips for Successful Cell Culture

Culturing cells in the lab? Following these tips will add to your success and help you avoid wasted ...
Dan D Apr 24, 2019

Tech Tips Video: How much antibody should I use for ChIP?

It's time to check out another video from the CST Tech Tips playlist! In this edition of Tech Tips, ...
Kenneth Buck, PhD Nov 28, 2018

Milk or BSA? How to Choose a Blocking Protein for Western Blot [Video]

So you've set the timer to five minutes for the first of three TBST washes for your western blot mem...
Kenneth Buck, PhD Sep 5, 2018

Keep ChIP’ing Away

So your experiments and data are funneling you down an inescapable path. You need to show direct gen...
Curtis Desilets Jan 31, 2018

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