Multiplex immunohistochemistry (mIHC) and multiplex immunofluorescence (mIF) are widely used to identify and localize different cell types within tissue samples. But choosing the right technique depends on the aims of the study and the tools available. A recent publication highlights the advantages and disadvantages of different approaches to mIHC/mIF and explains how these methods differ1.
Critical Considerations for Optimizing Imaging Mass Cytometry
Posted by Chris S on Nov 20, 2019 3:15:00 AM
Understanding the microenvironment of disease is critical for the success of targeted therapies. For years, the complex landscape of the tumor microenvironment could only be studied in single-cell snapshots by flow cytometry or limited tissue staining. Advanced multiplexing technologies such as Imaging Mass Cytometry™ (IMC™) now enable the imaging of up to 40 protein markers on both the cellular and tissue level.
Topics: IHC
Imaging Mass Cytometry: Endless possibilities to understand the microenvironment of disease
Posted by Kris H on Oct 23, 2019 3:15:00 AM
Understanding the microenvironment of disease is critical. Targeted cancer therapies may not be effective depending on the combination of cell types in the tumor. Likewise, an immune cell invasion in pancreatic tissue could indicate the progression of type 1 diabetes. For years, this complex landscape could only be witnessed in single-cell snapshots by flow cytometry or limited tissue staining. Imaging Mass Cytometry™ (IMC™) now offers a panorama of this dynamic world.
Topics: IHC, techniques
For decades, immunohistochemistry (IHC) has been a powerful technique for the investigation and visualization of cellular components in their native histological context. IHC has served as an important tool in medicine – enabling the diagnosis of complex pathological conditions – and in basic research to advance the understanding of key biological processes.
Topics: IHC, Immunology
Visualize and Understand Protein Co-expression during EMT
Posted by Tamar A. on Aug 7, 2019 3:10:00 AM
Careful planning and the fine tuning of experimental protocols are key to ensuring clear, interpretable scientific results. This is especially true for immunohistochemistry (IHC) studies, where each step in the often multiday process – from tissue preparation to stain development – can significantly impact the final outcome and analysis. Often, the simultaneous examination of multiple antigens is required to address specific scientific questions, which further complicates IHC protocol development. A general understating of the steps necessary to optimize IHC for multiple targets is essential to achieve reliable results. So, what are these steps?
Topics: IHC
Video: Can you trust your immunohistochemistry results?
Posted by Chris S on Jun 19, 2019 3:05:00 AM
Immunohistochemistry, or IHC, remains the simplest method for detecting biomarker expression while maintaining spatial context within tissues. You know that getting reliable IHC staining results hinges on the specificity and performance of your antibody. These are high stakes experiments, and you want to be 100% confident your antibody will detect the target of interest.
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Topics: IHC
If I could only see what’s going on inside your brain...
Posted by Richard C on Jun 5, 2019 3:15:00 AM
For generations of neuroscientists, using immunohistochemistry to study the brain in its anatomical context typically meant imaging a tiny slice at a time. Using the traditional method of taking micron thick sections, fixing, staining, imaging, and, finally, stitching all the slices together, is a super laborious task, especially for large tissues. But what if you could "look" into an intact mouse brain and identify specific cells and eliminate all of the slicing and stitching?
Topics: IHC, Alzheimer's Disease, Neurodegeneration
In recent years, immune checkpoint proteins in the tumor microenvironment have been under intense study. If you work in the immuno-oncology field, chances are you are either performing multiplex IHC (mIHC) or would like to. Ultimately, a multiplexed image like the one featured here provides a multi-layered depiction of a tumor, such that each antibody corresponds to a different fluorescent signal. If you want to detect more targets in your IHC, but aren’t sure how to design a panel of antibodies and fluorophores for mIHC, we’ll walk you through the process in this post.
Topics: IHC, Cancer Research, mIHC, techniques, Immunology
Switching from Manual to Automated IHC? We've got your back
Posted by Chris G on Aug 30, 2017 3:00:00 AM
If you’ve ever transitioned your IHC experiments from a manual protocol to an automated platform, you may have found the conversion process to be a drag. It isn’t an easy thing to do. For that reason, we’re happy to announce our IHC Leadership in Automation initiative. This rigorous validation initiative expands on our already thorough measures, allowing researchers to not only use CST products with our recommended manual IHC protocol, but also to bridge the assay to new platforms and techniques. Our foray into the world of automated IHC aims to reduce the amount of time researchers spend on assay transfer and protocol optimization.
Topics: Protocols, IHC, Antibody Performance, Antibody Validation, techniques, Automated IHC
Webinar | Highly Multiplexed Single Cell Analysis of Tumor Heterogeneity through Time and Space by Mass Cytometry
Posted by Chris S on Jun 21, 2017 9:38:58 AM
The study of the tumor ecosystem and its cell-to-cell communications is essential to enable an understanding of tumor biology, to define new biomarkers to improve patient care, and ultimately to identify new therapeutic routes and targets.
Topics: IHC