CST BLOG: Lab Expectations

The official blog of Cell Signaling Technology® (CST) where we discuss what to expect from your time at the bench, share tips, tricks, and information.

Trim-Away: Rapid, Easy, and Specific Protein Degradation

Foundational approaches for studying protein function in living models—gene editing and RNA interfer...

Post-Translational Modifications: Analyzing Protein Activation State

Post-translational modifications (PTMs) are a critical regulatory mechanism that allows cells to tig...
Andrea T Dec 8, 2021

A Tale of Two Stains: Ponceau S vs. Coomassie Blue

You've just finished running your lysate sample by SDS-PAGE, so you're pretty confident your protein...

Recommended Best Practices for Lysate Handling

The preparation of a cell lysate is crucial to the success of many assays, including techniques that...
Michelle G Sep 2, 2020

Mouse Tissue Loading Controls

When it comes to mouse tissue samples, not all loading control proteins are expressed equally. For e...
Supriya S Jun 24, 2020

4 Situations Where Flow Cytometry Might Be More Useful Than Western Blotting

Western blotting is used to detect proteins and analyze their expression using antibodies, but it do...
Chris S Dec 4, 2019

Tech Tips Video: Milk or BSA? Choosing a blocking protein for Western Blot

So you've set the timer to five minutes for the first of three TBST washes for your western blot mem...
Ken B Sep 5, 2018

Are you ready to transfer your protein? Wet vs. semi-dry transfer

 You are all amped up to run a western blot to identify “your favorite protein.” The lysates have be...
Neha G Apr 18, 2018

Have You Ever Wondered...How Do I Choose a Loading Control?

For those who wish to bring a measure of rationality to the western blot data in the literature, loa...
Chris S Mar 21, 2018

Have you ever wondered: Am I setting up my loading controls correctly?

You’re gathering data from all your experiments and preparing to present to your advisor and thesis ...
Ken B Nov 1, 2017

Have You Ever Wondered: What's The Lowdown on Phospho-Specific Antibodies?

Early exploration of unmapped biological signaling pathways were carried out using radiolabeled phos...
Ken B Jul 26, 2017

The Cost of a Failed Western Blot

Thinking about lab expenses isn’t as enjoyable as investigating your favorite signaling pathway. How...
Claire S Aug 13, 2015

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