CST BLOG: Lab Expectations

The official blog of Cell Signaling Technology (CST) where we discuss what to expect from your time at the bench, share tips, tricks, and information.

Lab Expectations listing page

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How to Control Your Apoptosis, Autophagy, and Mitophagy Western Blots

Have you ever spent time treating cells, preparing cell extracts, and performing a western blot only...
Whitney Palmer Dec 4, 2023

Identify New Immuno-Oncology Strategies Faster with Rapid, One-Step T Cell Activation & Expansion Kits

The success of immuno-oncology therapeutics has opened the door to novel cancer elimination strategi...
Andrea Tu, PhD Sep 20, 2023

Introducing Validated Antibodies for Simple Western–An Automated Capillary Electrophoresis Immunoassay

Guest blogger Charles Haitjema, PhD, is a Research Area Manager at Bio-Techne. This blog post detail...
Charles Haitjema, PhD Feb 22, 2023

How do I choose a loading control for my western blot?

Think about the last time you generated western blot data. Did you find the predicted differences in...
Supriya Singh, PhD Feb 1, 2023

Analyzing PTM Activation State Using Western Blotting

Post-translational modifications (PTMs) are a critical regulatory mechanism that allows cells to tig...
Andrea Tu, PhD Dec 8, 2021

A Tale of Two Stains: Ponceau S vs Coomassie Blue

You've just finished running your lysate sample by SDS-PAGE, so you're pretty confident your protein...

Recommended Best Practices for Lysate Handling

The preparation of a cell lysate is crucial to the success of many assays, including techniques that...
Michelle G Sep 2, 2020

Loading Control Expression in Mouse Tissues

When it comes to mouse tissue samples, not all western blot loading control proteins are expressed e...
Supriya Singh, PhD Jun 24, 2020

4 Situations Where Flow Cytometry Might Be More Useful Than Western Blotting

Western blotting is used to detect proteins and analyze their expression using antibodies, but it do...
Chris Sumner Dec 4, 2019

Milk or BSA? How to Choose a Blocking Protein for Western Blot [Video]

So you've set the timer to five minutes for the first of three TBST washes for your western blot mem...
Kenneth Buck, PhD Sep 5, 2018

Are you ready to transfer your protein? Wet vs. semi-dry transfer

You are all amped up to run a western blot to identify “your favorite protein.” The lysates have bee...
Neha G Apr 18, 2018

Western Blot Normalization: Am I setting up my loading controls correctly?

You’re gathering data from all your experiments and preparing to present to your advisor and thesis ...
Kenneth Buck, PhD Nov 1, 2017

Have You Ever Wondered: What's The Lowdown on Phospho-Specific Antibodies?

Early exploration of unmapped biological signaling pathways was carried out using radiolabeled phosp...
Kenneth Buck, PhD Jul 26, 2017

The Cost of a Failed Western Blot

Thinking about lab expenses isn’t as enjoyable as investigating your favorite signaling pathway. How...
Claire S Aug 13, 2015

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