CST BLOG: Lab Expectations

The official blog of Cell Signaling Technology (CST), where we discuss what to expect from your time at the bench, share tips, tricks, and information.

Lab Expectations listing page

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500 Antibodies & Counting: Accelerating Discovery with CST Antibodies Validated for Simple Western Technology

Since 2022, Cell Signaling Technology (CST) and Bio-Techne have been working together to bring best-...
Chris LaBreck, PhD Oct 23, 2024

Validating a Monoclonal Antibody for the ADP-Ribosylation PTM

Protein adenosine diphosphate, or ADP-ribosylation, is a fascinating post-translational modification...

Unraveling Alzheimer’s Disease: Developing a Phospho-Tau 217 Antibody

With Alzheimer’s disease (AD) set to affect more than 7.1 million people over 65 worldwide by 2025, ...
Arica Aiello Aug 14, 2024

How to Control Your Apoptosis, Autophagy, and Mitophagy Western Blots

Have you ever spent time treating cells, preparing cell extracts, and performing a western blot only...
Whitney Palmer Dec 4, 2023

Faster Immuno-Oncology Research with Rapid T Cell Activation & Expansion

The success of immuno-oncology therapeutics has opened the door to novel cancer elimination strategi...
Andrea Tu, PhD Sep 20, 2023

Got the 'NOS'? Fueling Research with New Nitro-Tyrosine Antibody Tools

Since 2000, Cell Signaling Technology (CST) has offered a rigorously tested and well-cited polyclona...

CST Antibodies Validated for Simple Western–An Automated Capillary Electrophoresis Immunoassay

Guest blogger Charles Haitjema, PhD, is a Research Area Manager at Bio-Techne. This blog post detail...
Charles Haitjema, PhD Feb 22, 2023

How do I choose a loading control for my western blot?

Think about the last time you generated western blot data. Did you find the predicted differences in...
Supriya Singh, PhD Feb 1, 2023

Analyzing PTM Activation State Using Western Blotting

Post-translational modifications (PTMs) are a critical regulatory mechanism that allows cells to tig...
Andrea Tu, PhD Dec 8, 2021

A Tale of Two Stains: Ponceau S vs Coomassie Blue

You've just finished running your lysate sample by SDS-PAGE, so you're pretty confident your protein...

Recommended Best Practices for Lysate Handling

The preparation of a cell lysate is crucial to the success of many assays, including techniques that...
Michelle Graham Sep 2, 2020

Control Your Western Blot: Loading Control Expression in Mouse Tissues

When it comes to mouse tissue samples, not all western blot loading control proteins are expressed e...
Supriya Singh, PhD Jun 24, 2020

10 Must-have Markers for Senescence Research

Understanding when and why cell cycle arrest occurs is critical to many fields of research, includin...
Tamar Aprahamian, PhD May 13, 2020

4 Situations Where Flow Cytometry Might Be More Useful Than Western Blotting

Western blotting is used to detect proteins and analyze their expression using antibodies, but it do...
Chris Sumner Dec 4, 2019

When should I use flow cytometry for signaling instead of western blot?

Flow cytometry enables quantitative analysis of protein expression, signaling states, and physical c...
Chris Sumner Jan 9, 2019

Milk or BSA? How to Choose a Blocking Protein for Western Blot [Video]

So you've set the timer to five minutes for the first of three TBST washes for your western blot mem...
Kenneth Buck, PhD Sep 5, 2018

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