CST BLOG: Lab Expectations

The official blog of Cell Signaling Technology® (CST) where we discuss what to expect from your time at the bench, share tips, tricks, and information.

Lab Expectations listing page

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Kenneth Buck, PhD

Kenneth Buck, PhD

Recent Posts:

Video: How to Choose Enzymatic or Sonication Protocol for ChIP

The success of your chromatin immunoprecipitation (ChIP) experiment depends on the fragmentation of ...
Kenneth Buck, PhD Nov 6, 2019

Tech Tips Video: How much antibody should I use for ChIP?

It's time to check out another video from the CST Tech Tips playlist! In this edition of Tech Tips, ...
Kenneth Buck, PhD Nov 28, 2018

Video: Understanding Macrophages in the Tumor Microenvironment

Research Trends in Macrophage Plasticity It’s an exciting time for immuno-oncology research, as pote...
Kenneth Buck, PhD Sep 19, 2018

Tech Tips Video: Milk or BSA? Choosing a blocking protein for Western Blot

So you've set the timer to five minutes for the first of three TBST washes for your western blot mem...
Kenneth Buck, PhD Sep 5, 2018

Q&A: From Signaling Pathways to Career Pathways

Each summer, college and high school interns at CST get a chance to sample work life in a biotech en...
Kenneth Buck, PhD Aug 15, 2018

Western Blot: Am I setting up my loading controls correctly?

You’re gathering data from all your experiments and preparing to present to your advisor and thesis ...
Kenneth Buck, PhD Nov 1, 2017

Have You Ever Wondered: What's The Lowdown on Phospho-Specific Antibodies?

Early exploration of unmapped biological signaling pathways were carried out using radiolabeled phos...
Kenneth Buck, PhD Jul 26, 2017

Successful Immunofluorescence: Antibody Dilution and Incubation Conditions

Part four of a series on immunofluorescence techniques. Check out previous posts on Validation, Expe...
Kenneth Buck, PhD May 10, 2017

Journal Club | Zika virus turns off Akt signaling to hijack autophagy in developing neural tissue

The Zika virus (ZIKV) is related to flaviviruses that cause dengue and yellow fever, and is spread v...
Kenneth Buck, PhD Mar 29, 2017

Successful Immunofluorescence: Fixation and Permeabilization

Part Three of a four-part series on immunofluorescence. Read Part 1: The Importance of Validation an...
Kenneth Buck, PhD Mar 15, 2017

Successful Immunofluorescence: Experimental Controls

 Part two of a four-part series on Immunofluorescence. Check out our posts on Validation and Fixatio...
Kenneth Buck, PhD Mar 8, 2017

Successful Immunofluorescence: The Importance of Validation

Part one of a four-part series on Immunofluorescence. Check out Experimental Controls, Fixation and ...
Kenneth Buck, PhD Mar 1, 2017

Season's Greetings from CST!

The holiday season is traditionally marked by festive choral music, decorated cookies, and in recent...
Kenneth Buck, PhD Dec 21, 2016

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