CST BLOG: Lab Expectations

The official blog of Cell Signaling Technology (CST), where we discuss what to expect from your time at the bench, share tips, tricks, and information.

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Milk or BSA? How to Choose a Blocking Protein for Western Blot [Video]

So you've set the timer to five minutes for the first of three TBST washes for your western blot mem...
Kenneth Buck, PhD Sep 5, 2018

Are you ready to transfer your protein? Wet vs Semi-dry Transfer

You are all amped up to run a western blot to identify “your favorite protein.” The lysates have bee...

Western Blot Normalization: Am I setting up my loading controls correctly?

You’re gathering data from all your experiments and preparing to present to your advisor and thesis ...
Kenneth Buck, PhD Nov 1, 2017

Fluorescent Staining Using Multiple Antibodies: Two Common Techniques

The use of multiple antibodies in a single experiment can provide useful information to researchers....
Tara White Sep 13, 2017

What Are Phospho-Specific Antibodies?

Early exploration of unmapped biological signaling pathways was carried out using radiolabeled phosp...
Kenneth Buck, PhD Jul 26, 2017

Successful Immunofluorescence: Antibody Dilution & Incubation Conditions

Part four of a four-part series on successful immunofluorescence. Read Part 1: The Importance of Val...
Kenneth Buck, PhD May 10, 2017

Successful Immunofluorescence: Fixation and Permeabilization

Part Three of a four-part series on successful immunofluorescence. Read Part 1: The Importance of Va...
Kenneth Buck, PhD Mar 15, 2017

Successful Immunofluorescence: Experimental Controls

Part two of a four-part series on successful immunofluorescence. Read Part 1: The Importance of Vali...
Kenneth Buck, PhD Mar 8, 2017

Successful Immunofluorescence: The Importance of Validation

Part 1 of a four-part series on successful immunofluorescence. Read Part 2: Experimental Controls, P...
Kenneth Buck, PhD Mar 1, 2017

The Questions to Ask Before Starting Your Next IF Experiment

Specificity, consistency, and optimized assay conditions are three key elements that help ensure rel...

Five Steps to Publication-Worthy IF Images

A picture is worth a thousand words, or in the case of immunofluorescent (IF) imaging, a thousand pr...
Tara White Sep 23, 2015

The Cost of a Failed Western Blot

Thinking about lab expenses isn’t as enjoyable as investigating your favorite signaling pathway. How...
Claire S Aug 13, 2015

Four Steps to Better Immunohistochemistry: Steps 2, 3, and 4

This is part two of a two-part series on how to optimize your immunohistochemistry (IHC) protocols. ...
Carolyn P Jul 15, 2015

Four Steps to Better Immunohistochemistry: Antigen Retrieval

It’s Friday night and you could be out with your friends right now, but instead you’re tucked away i...
Carolyn P Jul 7, 2015

From PCR to ChIP-seq: How to Analyze Your ChIP Data

Part one of this series described the importance of including proper controls in your protocols. In ...
Carolyn P Mar 11, 2015

Signal vs Noise: Choosing the Right Antibody for Successful ChIP

Part one of this series described the importance of including proper controls to your protocols. In ...
Carolyn P Mar 4, 2015

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