CST BLOG: Lab Expectations

The official blog of Cell Signaling Technology (CST), where we discuss what to expect from your time at the bench, share tips, tricks, and information.

Lab Expectations listing page

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Switching from Manual to Automated IHC? We've got your back

If you’ve ever transitioned your IHC experiments from a manual protocol to an automated platform, yo...
Chris G Aug 30, 2017

Have You Ever Wondered: What's The Lowdown on Phospho-Specific Antibodies?

Early exploration of unmapped biological signaling pathways was carried out using radiolabeled phosp...
Kenneth Buck, PhD Jul 26, 2017

4 Steps to Better Immunohistochemistry: Steps 2, 3, and 4

This is part two of a two-part series on how to optimize your IHC protocols. Part one introduced the...
Carolyn P May 31, 2017

Four Steps to Better Immunohistochemistry: Step 1 - Antigen Retrieval

It’s Friday night and you could be out with your friends right now, but instead you’re tucked away i...
Carolyn P May 24, 2017

Successful Immunofluorescence: Antibody Dilution & Incubation Conditions

Part four of a four-part series on successful immunofluorescence. Read Part 1: The Importance of Val...
Kenneth Buck, PhD May 10, 2017

Successful Immunofluorescence: Fixation and Permeabilization

Part Three of a four-part series on successful immunofluorescence. Read Part 1: The Importance of Va...
Kenneth Buck, PhD Mar 15, 2017

Successful Immunofluorescence: Experimental Controls

Part two of a four-part series on successful immunofluorescence. Read Part 1: The Importance of Vali...
Kenneth Buck, PhD Mar 8, 2017

Successful Immunofluorescence: The Importance of Validation

Part 1 of a four-part series on successful immunofluorescence. Read Part 2: Experimental Controls, P...
Kenneth Buck, PhD Mar 1, 2017

Immunofluorescence: The Questions to Ask Before Starting Your Next Experiment

Specificity, consistency, and optimized assay conditions are three key elements that help ensure rel...
Liana G Oct 19, 2016

Three Questions to Ask Before Starting Your Next Immunofluorescence Experiment

Specificity, consistency, and optimized assay conditions are three key elements that help ensure rel...
Carolyn P Sep 7, 2016

Five Steps to Publication-Worthy IF Images

A picture is worth a thousand words, or in the case of immunofluorescent (IF) imaging, a thousand pr...
Tara White Sep 23, 2015

The Cost of a Failed Western Blot

Thinking about lab expenses isn’t as enjoyable as investigating your favorite signaling pathway. How...
Claire S Aug 13, 2015

Intracellular Flow Cytometry in Action

Traditionally, flow cytometry has been used to identify distinct cell types within a heterogeneous p...
Liana G Aug 12, 2015

Need Better ChIP Results? Use Enzymatic Digestion

The success or failure of a chromatin immunoprecipitation (ChIP) experiment is highly dependent on t...
Carolyn P Aug 5, 2015

Four Steps to Better Immunohistochemistry: Steps 2, 3, and 4

This is part two of a two-part series on how to optimize your immunohistochemistry (IHC) protocols. ...
Carolyn P Jul 15, 2015

4 Steps to Better ChIP results - Step 4 - Downstream Analysis

Part one of this series described the importance of including proper controls to your protocols. In ...
Carolyn P Mar 11, 2015

4 Steps to Better ChIP results - Step 3- Choosing an Antibody

Part one of this series described the importance of including proper controls to your protocols. In ...
Carolyn P Mar 4, 2015

4 Steps to Better ChIP results - Step 2- Prep well

Part one of this series described the importance of including proper controls in your protocols. Now...
Carolyn P Feb 25, 2015

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