CST BLOG: Lab Expectations

The official blog of Cell Signaling Technology (CST), where we discuss what to expect from your time at the bench, share tips, tricks, and information.

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Carolyn P

Carolyn P

Recent Posts:

Reproducibility in Research Science - Join the Discussion

Lack of reproducibility in scientific studies has been a major topic of conversation lately. It's an...
Carolyn P Jun 28, 2017

4 Steps to Better Immunohistochemistry: Steps 2, 3, and 4

This is part two of a two-part series on how to optimize your IHC protocols. Part one introduced the...
Carolyn P May 31, 2017

Four Steps to Better Immunohistochemistry: Step 1 - Antigen Retrieval

It’s Friday night and you could be out with your friends right now, but instead you’re tucked away i...
Carolyn P May 24, 2017

How to Validate a Histone Modification Antibody

Antibodies targeted to histone modifications may bind non-specifically to similar, but off-target hi...
Carolyn P Feb 1, 2017

Three Questions to Ask Before Starting Your Next Immunofluorescence Experiment

Specificity, consistency, and optimized assay conditions are three key elements that help ensure rel...
Carolyn P Sep 7, 2016

The Reproducibility Crisis: An Open Letter from Our Chief Scientific Officer

A Message from our Chief Scientific Officer, Roberto Polakiewicz, Ph.D. The time is now for the scie...
Carolyn P Aug 10, 2016

Investing in Our Communities and Promoting Science Education

If you want to build a ship, don't drum up the men to gather wood, divide the work and give orders. ...
Carolyn P Jul 20, 2016

History of the Monoclonal Antibody - Part I

Monoclonal antibodies are a pretty ubiquitous research tool. In fact, you’d be hard-pressed to find ...
Carolyn P Aug 26, 2015

Need Better ChIP Results? Use Enzymatic Digestion

The success or failure of a chromatin immunoprecipitation (ChIP) experiment is highly dependent on t...
Carolyn P Aug 5, 2015

Four Steps to Better Immunohistochemistry: Steps 2, 3, and 4

This is part two of a two-part series on how to optimize your immunohistochemistry (IHC) protocols. ...
Carolyn P Jul 15, 2015

4 Steps to Better ChIP results - Step 4 - Downstream Analysis

Part one of this series described the importance of including proper controls to your protocols. In ...
Carolyn P Mar 11, 2015

4 Steps to Better ChIP results - Step 3- Choosing an Antibody

Part one of this series described the importance of including proper controls to your protocols. In ...
Carolyn P Mar 4, 2015

4 Steps to Better ChIP results - Step 2- Prep well

Part one of this series described the importance of including proper controls in your protocols. Now...
Carolyn P Feb 25, 2015

4 Steps to Better ChIP results - Step 1- Take control

You're a confocal maestro, playing the lasers and the filters and the gain settings like a Stradivar...
Carolyn P Feb 18, 2015

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