Hallmarks of Validation - Ranged Strategy


Posted by Katie C on Feb 19, 2020 3:15:00 AM

While a binary approach is one of the more desirable ways to evaluate antibody specificity, binary models are not always readily available and can be time consuming or expensive to produce for the sole purpose of validating an antibody. Moreover, to assess the sensitivity of an antibody in the application and protocol being used, a complementary hallmark is required.

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Topics: Antibody Performance, Antibody Validation

Hallmarks of Antibody Validation - Binary Strategy


Posted by Katie C on Jan 22, 2020 3:00:00 AM

A binary approach is one of the best ways to evaluate antibody specificity. By testing an antibody in biologically relevant positive and negative expression systems, it is possible to confirm that it  recognizes the target antigen in its native environment without cross-reacting with other biomolecules present in the sample.

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Topics: Antibody Performance, Antibody Validation

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