CST BLOG

The official blog of Cell Signaling Technology, where we discuss what to expect from your time at the bench, share tips, tricks, and information.

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ChIP, CUT&RUN & CUT&Tag: Support Reagents for Successful Chromatin Profiling

If you’ve performed CUT&RUN, CUT&Tag, or ChIP/ChIP-seq experiments, you know how important i...
Andrea Tu, PhD May 8, 2024

How to Accurately Count Cells Manually or with an Automated Cell Counter

There are several situations in the lab where you’ll need to know the number of cells in a given vol...
Kenneth Buck, PhD May 17, 2023

Trim-Away: Rapid, Easy, and Specific Protein Degradation

Foundational approaches for studying protein function in living models—gene editing and RNA interfer...

Comparing Antibody Concentrations & Performance; CST Antibody Details You May Not Know

When purchasing antibodies from Cell Signaling Technology (CST), you may notice that we typically re...
Josh Nathan Oct 12, 2022

Which Fluorophores Can I Combine in My Flow Cytometry Experiment?

Flow cytometry offers the exciting ability to detect and quantify multiple readouts per cell, enabli...
Rob MacDonald, PhD Apr 13, 2022

Subcellular Marker Selection for Immunofluorescence

When performing immunofluorescence (IF) experiments, researchers often multiplex with several antibo...
Ginny Bain, PhD Jan 12, 2022

Why use viability dyes in flow cytometry experiments?

Viability dyes are widely used when counting and passaging cells, assessing chemical toxicity, and p...
Rob MacDonald, PhD Nov 17, 2021

Tools for Modulating Cells

Cell behavior is a tightly regulated, finely tuned process where various different signals work toge...
Andrea Tu, PhD Oct 13, 2021

Assay Kits and Tools for Monitoring Cell State

Have you been wondering how to monitor a cell state or cellular response? From a basic research stan...
Andrea Tu, PhD Oct 6, 2021

How S6 Ribosomal Protein Rabbit Antibody Can Help with Your IF Staining

One antibody is used in our lab so often that we each keep our own bulk stock! This antibody is S6 R...

A Tale of Two Stains: Ponceau S vs Coomassie Blue

You've just finished running your lysate sample by SDS-PAGE, so you're pretty confident your protein...

Western Blotting: Best Practices for Cell Lysate Preparation

The preparation of a cell lysate is crucial to the success of many assays, including techniques that...
Michelle Graham Sep 2, 2020

Cell Process: How is cytotoxicity assessed?

Changes in cellular health in response to exogenous stimuli can provide keen insight into the biolog...
Tamar Aprahamian, PhD Jul 8, 2020

Seven Assays to Detect Apoptosis

Apoptosis is a highly regulated form of programmed cell death that occurs in multicellular organisms...
Tamar Aprahamian, PhD Jul 1, 2020

Control Your Western Blot: Loading Control Expression in Mouse Tissues

When it comes to mouse tissue samples, not all western blot (WB) loading control proteins are expres...
Supriya Singh, PhD Jun 24, 2020

Antibody Selection for Epitope Tagging: Insights from Myc-Tag Performance

Biological research is about expanding knowledge, so it's not uncommon for scientists to study prote...
Tamar Aprahamian, PhD May 20, 2020

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