Hallmarks of Validation - Ranged Strategy


Posted by Katie C on Feb 19, 2020 3:15:00 AM

While a binary approach is one of the more desirable ways to evaluate antibody specificity, binary models are not always readily available and can be time consuming or expensive to produce for the sole purpose of validating an antibody. Moreover, to assess the sensitivity of an antibody in the application and protocol being used, a complementary hallmark is required.

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Topics: Antibody Performance, Antibody Validation

Immunology: How does the innate immune system work?


Posted by April L on Feb 12, 2020 3:15:00 AM

As we touched upon in a previous blog post, the innate immune system is an important part of our bodies’ immune defenses and it provides us with a general protective response against microbial invaders and foreign proteins. This innate immune system is evolutionarily older than our adaptive immune system, with conserved mechanisms seen in plants, animals, and insects, and it is not specialized against any particular pathogen.

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Topics: Immunology

Masks and Protective Clothing Needed to Fight Coronavirus: How can we help?


Posted by April L on Feb 5, 2020 3:00:00 AM

We at Cell Signaling Technology (CST) have been paying close attention to news about the recent and rapid spread of a new form of coronavirus in China. As members of a global organization with many esteemed colleagues in China and all over the world, we are concerned for all who are affected by this terrible respiratory virus and are eager to provide aid to those in need.

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Topics: Corporate Social Responsibility

Hallmarks of Antibody Validation - Binary Strategy


Posted by Katie C on Jan 22, 2020 3:00:00 AM

A binary approach is one of the best ways to evaluate antibody specificity. By testing an antibody in biologically relevant positive and negative expression systems, it is possible to confirm that it  recognizes the target antigen in its native environment without cross-reacting with other biomolecules present in the sample.

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Topics: Antibody Performance, Antibody Validation

Introduction to the Hallmarks of Antibody Validation


Posted by Chris S on Jan 15, 2020 3:00:00 AM

Antibodies are essential reagents that support all levels of scientific research. Used in a multitude of applications to identify, quantify, and isolate specific target biomolecules, they have recently become the focus of intense scrutiny for their contribution to the ongoing reproducibility crisis.

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Topics: Antibody Performance, Antibody Validation

Immunology Overview: How does our immune system protect us?


Posted by April L on Jan 8, 2020 3:15:00 AM

Scientists and medical professionals have been studying what makes us sick and the myriad mechanisms the body uses to respond to illness for hundreds of years. Some of these mechanisms are quite simple and some are elegantly complex. In this first installment of the Immunology blog series we will review the different strategies the immune system employs to keep diseases at bay.

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Topics: Immunology

Coming to Lab Expectations in 2020


Posted by Chris S on Jan 1, 2020 3:15:00 AM

            The start of a new year is always a crazy time. There’s plenty to look back on and celebrate, things to anticipate, and of course there are opportunities for improvement. What do we look forward to in 2020? We’re about to celebrate our 5th blog anniversary! It’s hard to believe that Lab Expectations is five years old.

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Topics: Science Education

4 Situations Where Flow Cytometry Might Be More Useful Than Western Blotting


Posted by Chris S on Dec 4, 2019 3:00:00 AM

Western blotting is used to detect proteins and analyze their expression using antibodies, but it does have its limitations. Flow cytometry also employs antibodies, but it uses fluorescence to detect and analyze protein expression on a cell-by-cell basis.

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Topics: Western Blot, Flow, techniques

Critical Considerations for Optimizing Imaging Mass Cytometry


Posted by Chris S on Nov 20, 2019 3:15:00 AM

Understanding the microenvironment of disease is critical for the success of targeted therapies. For years, the complex landscape of the tumor microenvironment could only be studied in single-cell snapshots by flow cytometry or limited tissue staining. Advanced multiplexing technologies such as Imaging Mass Cytometry™ (IMC™) now enable the imaging of up to 40 protein markers on both the cellular and tissue level.

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Topics: IHC

Video: How to Choose Enzymatic or Sonication Protocol for ChIP


Posted by Ken B on Nov 6, 2019 3:00:00 AM

The success of your chromatin immunoprecipitation (ChIP) experiment depends on the fragmentation of chromatin, a critical step in the ChIP protocol. This can be accomplished with either sonication or enzymatic digestion. But how do you decide which chromatin fragmentation protocol to use in your ChIP experiments? A number of factors can influence your choice, making a decision seem daunting. So let’s simplify – watch the video and we'll will show you the way!

 

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Topics: Protocols, ChIP, techniques

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