Intracellular Flow Cytometry in Action


Posted by Liana G on Sep 21, 2016 3:00:00 AM


Traditionally, flow cytometry has been used to identify distinct cell types within a heterogeneous pool of cells, based on extracellular or surface marker expression, an application commonly known as immuno-phenotyping. However, this technology is also readily amenable to intracellular target detection and can be successfully applied to the study of complex signaling events.

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Topics: Protocols, Flow, Cell Biology, techniques

The Cost of a Failed Western Blot


Posted by Claire S on Sep 14, 2016 3:00:00 AM


Thinking about lab expenses isn’t as enjoyable as investigating your favorite signaling pathway. However, because research money is hard to come by, it is something that should be considered when picking your reagents for western blotting. It makes sense to keep the quality of your primary antibody in mind, because the success of the entire experiment depends on the antibody being reliable, specific, and sensitive. If the antibody does not perform as expected your experiment may fail . . . and the cost of a failed western blot may be more than you think.

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Topics: Antibody Performance, Antibody Validation, Western Blot, techniques, Reproducibility

3 Questions to ask before starting your next immunofluorescence experiment.


Posted by Carolyn P on Sep 7, 2016 3:00:00 AM

Specificity, consistency, and optimized assay conditions are three key elements that help ensure reliable immunofluorescence (IF) staining results each and every time. So, before starting an experiment you should ask yourself the following three questions:

  • Is your antibody specific?
  • Is your antibody supported by an optimized IF protocol?
  • Is your antibody performing consistently?

Let's take them each in turn...

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Topics: Protocols, Primary Antibodies, Antibody Validation, IF-IC, Cell Biology, techniques

4 Steps to Better Immunohistochemistry: Steps 2, 3, and 4


Posted by Carolyn P on Aug 31, 2016 3:00:00 AM

This is part two of a two-part series on how to optimize your IHC protocols. Part one introduced the principles behind antigen retrival. Click here if you missed it...but, if you've got your tissue prepped and ready to go, we'll move on to the next steps in the staining protocol.

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Topics: Protocols, IHC, techniques

4 Steps to Better Immunohistochemistry: Step 1 - Antigen Retrieval


Posted by Carolyn P on Aug 24, 2016 3:00:00 AM

It’s Friday night and you could be out with your friends right now, but instead you’re tucked away in a dark little room filled with microscopes. Spending the evening in the lab seemed like a good choice at the time because you were certain this immunohistochemistry was going to reveal some small - but important - mystery of the universe to you.

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Topics: Protocols, IHC, Companion Reagents, techniques

Webinar | Targeting Cancer Pathways: Understanding Immune Checkpoints


Posted by Carolyn P on Aug 17, 2016 3:00:00 AM

 
In this webinar, the speakers will explore how tumors exploit immune modulatory mechanisms to generate and thrive in their own immunosuppressive microenvironment. In addition, they will:
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Topics: Cancer Immunology, Cancer Research, Webinars

The Reproducibility Crisis: An open letter from CST's Chief Scientific Officer


Posted by Carolyn P on Aug 10, 2016 3:00:00 AM

 

A Message from our Chief Scientific Officer, Roberto Polakiewicz, Ph.D.

The time is now for the scientific community to come together to respond to the challenges of reproducibility and to be part of the solution . . . 

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Topics: Reproducibility

Reproducibility in Research Science - Join the Discussion.


Posted by Carolyn P on Aug 3, 2016 3:00:00 AM

Lack of reproducibility in scientific studies has been a major topic of conversation lately. It's an important conversation for us to have, given the potential of this problem to affect how the research community views the published literature.  Many potential reasons for the problem exist, but CST is focused on the role vendors play in ensuring the reagents being used by the scientific community are properly validated. 

These are important issues that need to be addressed by the scientific community and we should all be part of the conversation . . . 

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Topics: Reproducility

Webinar | Key Signaling Pathways in Cancer: Links to Developmental Biology


Posted by Carolyn P on Jul 27, 2016 3:00:00 AM

It is notable that the same pathways governing the cell growth, death, and differentiation decisions made during embryonic development are also common drivers of adult malignancy. In this webinar, we will explore the idea that a better understanding of developmental biology signaling pathways will advance our understanding of adult tumors and cancer stem cells as well as boost our ability to create effective therapeutics to fight a broad array of cancers.

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Topics: Developmental Biology, Webinars

Investing in our Communities and Promoting Science Education


Posted by Carolyn P on Jul 20, 2016 3:00:00 AM

If you want to build a ship, don't drum up the men to gather wood, divide the work and give orders. Instead, teach them to yearn for the vast and endless sea.
- Antoine de St. Exupery

Every generation has its dreamers; pioneering individuals who aren’t satisfied with the status quo, but who will drive forward towards something bigger, something better . . . 

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Topics: CSR