Seeding Labs


Posted by Chris S on Sep 23, 2020 3:00:00 AM

As stewards of the planet, we’re always trying to find ways to reduce our corporate carbon footprint and offset the environmental damage done through research. Operating a lab requires a lot of electricity: powering the freezers, microscopes, autostainers, and so much more. It all adds up. If you’re like us, you also want to find a way to give back, beyond the good that your research provides.

READ MORE >

Topics: Sustainability

Cell Process: What role do the telomeres play in senescence?


Posted by Tamar A. on Sep 16, 2020 3:00:00 AM

Senescence is a cellular state during which cells remain metabolically active, but irreversibly withdraw from the cell cycle and fail to respond to proliferation-inducing stimuli. Senescent cells influence a number of physiological and pathological processes from cancer to diabetes and aging. Accordingly, understanding why senescence contributes to these conditions may lead to the development of pro- and anti-senescence therapies to treat a range of diseases.

READ MORE >

Topics: Cell Biology

Cell Process: How is cellular senescence related to aging?


Posted by Tamar A. on Sep 9, 2020 3:00:00 AM

Senescence, the cessation of cell division and permanent withdrawal from the cell cycle, is a process that occurs throughout the lifespan — during embryogenesis, growth and development, tissue remodeling, and in wound healing. Senescent cells increase in number during aging and have been implicated in the decline of organismal function over time, as well as in the progression of age related diseases. These include metabolic and cardiovascular disorders, neurodegenerative disease, and cancer. In mammals, aging causes the gradual dysfunction of multiple tissue systems in a heterogeneous fashion, ultimately leading to death. Understanding how and why senescence contributes to the aging process may lead to therapies to slow or even reverse its progression.

READ MORE >

Topics: Cell Biology

Recommended Best Practices for Lysate Handling


Posted by Michelle G on Sep 2, 2020 3:00:00 AM

The preparation of a cell lysate is crucial to the success of many assays, including techniques that use antibodies to characterize protein expression. Ideally, throughout the whole process of sample preparation, from lysate collection to analysis, your protein of interest would be well-preserved and protected from the activities of proteases and phosphatases. Proper storage and handling of the lysate are crucial to avoiding degradation and maintaining the ability to detect your protein.

READ MORE >

Topics: Western Blot, techniques, Tech Tips

Cell Process: What signaling pathways are associated with cellular senescence?


Posted by Tamar A. on Aug 26, 2020 3:00:00 AM

Senescence is the irreversible arrest of proliferation in response to a variety of cellular stressors. This state is associated with changes in intracellular signaling pathways, as well that the secretion of proteins that affect the surrounding tissue microenvironment. Most notably, senescent cells exhibit a persistent DNA damage response, the activation of proteins which control cell cycle arrest, and the senescence associated secretory phenotype. Senescent cells are also resistant to apoptosis and demonstrate altered metabolic activity. Determining why changes in cellular signaling lead to senescence is key to understanding how senescence contributes to normal and pathological processes affecting human health.

READ MORE >

Topics: Cell Biology

PI3K/Akt Signaling Targets Hit Close to Home


Posted by Srikanth S on Aug 19, 2020 3:00:00 AM

The ability of cells to recognize external signal cues (ligands and nutrients) and appropriately respond to a rapidly changing environment involves crosstalk among major cellular signaling networks (1-2). These complex pathways sense intracellular nutrients, metabolic flux, and stress and integrate these distinct signals through numerous downstream effectors (1-2). As a scientist, I find trying to understand the intricate nature of pathways (where they overlap and intersect) to be an interesting puzzle to tease apart.

READ MORE >

Topics: Cell Biology

Cell Process: What is cell senescence?


Posted by Tamar A. on Aug 12, 2020 3:00:00 AM

Cellular senescence is a state of stable cell cycle arrest under which cells remain metabolically active, but no longer divide and do not respond to growth-promoting stimuli. Senescence is triggered by a variety of cellular stressors. These include environmental factors like ionizing radiation or exposure to chemotherapeutic drugs, oxidative stress, DNA damage, mitochondrial dysfunction, and oncogene activation. This process provides a defense mechanism to maintain tissue homeostasis through the sequestration of damaged cells. Senescent cells influence a number of physiological and pathological processes from cancer to diabetes and aging. Accordingly, understanding why senescence contributes to these conditions may lead to the development of pro- and anti-senescence therapies to treat a range of diseases.

READ MORE >

Topics: Cell Biology

How can recombinant proteins help researchers study SARS-CoV-2?


Posted by Tamar A. on Aug 5, 2020 3:15:00 AM

Although coronaviruses have long circulated throughout human populations, the study of these viruses has intensified over the last two decades, due to the rise of novel coronaviruses that have greatly impacted human health. The emergence of severe acute respiratory syndrome coronavirus (SARS-CoV) in 2003, Middle East respiratory syndrome-related coronavirus (MERS-CoV) in 2012, and the recent severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in 2019, has emphasized the importance of understanding the fundamental mechanisms by which these viruses cause disease.

READ MORE >

Topics: Immunology

Immunology: How does the adaptive immune system work?


Posted by April L on Jul 29, 2020 3:00:00 AM

Have you ever wondered how people are able to develop immunity to specific diseases? This important question has been the focus of many critical medical and immunological research endeavors. The answer is that it’s all due to the cells and antibodies of the adaptive immune system.

READ MORE >

Topics: Immunology

Pros and Cons of Different Multiplexing Techniques


Posted by Emma E on Jul 22, 2020 3:00:00 AM

Multiplex immunohistochemistry (mIHC) and multiplex immunofluorescence (mIF) are widely used to identify and localize different cell types within tissue samples. But choosing the right technique depends on the aims of the study and the tools available. A recent publication highlights the advantages and disadvantages of different approaches to mIHC/mIF and explains how these methods differ1.

READ MORE >

Topics: IHC, mIHC

Subscribe to Email Updates

Recent Posts