When should I use flow cytometry for signaling instead of western blot?


Posted by Chris S on Jan 9, 2019 3:15:00 AM

Flow cytometry enables quantitative analysis of protein expression, signaling states, and physical characteristics (cell size/granularity) at the single-cell level. Modern flow cytometers are capable of collecting data on multiple proteins from thousands of cells per second in a heterogeneous mixture. While flow cytometry is commonly employed to identify cell types using phenotypic markers expressed on the cell surface, it can also be used to measure intracellular signaling events.

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Topics: techniques, Flow

Reflecting on the New Year with Janus and Jak


Posted by Ken B on Jan 2, 2019 10:45:49 AM

In Roman mythology, the New Year and the month of January are associated with Janus, the god of transitions and doorways (1). Janus is best known for having two faces: one looking to the past, and one to the future. Janus also lends his name to the Janus kinase (Jak) family of nonreceptor tyrosine kinases.

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Topics: Immunology, Just for fun

Mycoplasma Contamination!


Posted by Michelle G on Dec 5, 2018 3:15:00 AM

Any form of cell culture contamination can ruin your day and destroy your hard work, but mycoplasma contamination is particularly devastating.

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Topics: Science Education, techniques

Tech Tips Video: How much antibody should I use for ChIP?


Posted by Ken B on Nov 28, 2018 3:15:00 AM

It's time to check out another video from the CST Tech Tips playlist! In this edition of Tech Tips, we'll tackle a common protocol question customers ask our ChIP team: how much antibody to use for chromatin immunoprecipitaion (ChIP) experiments. Adding more antibody isn't always better - watch the video to learn why. 

Can't see the embedded video above? Click the link below to play: 

How much antibody should I use in ChIP assays? | CST Tech Tips
 

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Topics: ChIP, Tech Tips, Protocols, Primary Antibodies

Webinar | Deciphering Neurodegeneration: Inflammation, immune response, and Alzheimer's


Posted by Chris S on Nov 14, 2018 3:15:00 AM

Hear about the potential role of innate immunity in neurodegeneration and cognitive function, particularly in Alzheimer's disease. Learn how immune-related pathways regulate the development, refinement, and elimination of specific axons and synapses during development. Gain an understanding of how recent work can provide insight into protecting synapses in neurodegenerative and psychiatric disorders of synaptic dysfunction.

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Topics: Webinars

Writing a Grant Part 4: Additional Details


Posted by Tamar A. on Nov 7, 2018 3:10:00 AM

Finally, the finish line is approaching! You have completed your specific aims, significance and innovation, and the bulk of your research strategy. You have sent those files for numerous rounds of pre-peer review by your trusted colleagues and mentors. Now it’s time to focus on some smaller, yet still very important details.

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Topics: Science Education, Career Development, techniques

Writing a Grant Part 3: The Experimental Approach


Posted by Tamar A. on Oct 31, 2018 3:10:00 AM

In the previous post, we described how to write an effective significance and innovation section, focused on defining the problem and providing a high-level overview of your proposed solution. In this post, we’ll outline the approach, wherein you’ll expand upon the solution and illustrate exactly how you plan to conduct the research.

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Topics: Science Education, techniques

Writing a Grant Part 2: Significance and Innovation


Posted by Tamar A. on Oct 24, 2018 7:43:37 AM

The significance and innovation section is a recent (within the last 10 years) addition to the NIH and most other foundation grant applications. It is a place for you to showcase WHY the work should be done – WHY there is a significant need for your study, and HOW the work is different from everyone else’s approach. What makes it groundbreaking, original research, work that will advance our scientific knowledge?

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Topics: Science Education, techniques

Writing a Grant Part 1: First Things First


Posted by Tamar A. on Oct 17, 2018 3:15:00 AM

So you’re thinking of writing a grant? Or maybe your mentor has politely suggested that it would be in your best interest to do so?

Where do you start?

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Topics: techniques, Career Development, Science Education

Demystifying Multiplex IHC


Posted by Jen Z on Oct 10, 2018 3:15:00 AM

In recent years, immune checkpoint proteins in the tumor microenvironment have been under intense study. If you work in the immuno-oncology field, chances are you are either performing multiplex IHC (mIHC) or would like to. Ultimately, a multiplexed image like the one featured here provides a multi-layered depiction of a tumor, such that each antibody corresponds to a different fluorescent signal. If you want to detect more targets in your IHC, but aren’t sure how to design a panel of antibodies and fluorophores for mIHC, we’ll walk you through the process in this post.

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Topics: Immunology, IHC, mIHC, Cancer Research, techniques

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