For antigenic targets where expression of the protein is very low or unknown, the use of recombinant proteins or exogenous expression in a surrogate cell line may be necessary for antibody validation. Although endogenous systems are preferred for their closer representation of in vivo conditions, heterologous strategy offers several advantages.
Fibrosis is a disease that is characterized by scarring and hardening of tissues and organs. Fibrosis can affect all tissues of the body, and left unchecked, can result in organ failure and death. What causes it? Believe it or not, it is a process that stems from wound healing that has gone awry.
Topics: Cell Biology
A multiple antibody strategy is a powerful approach to antibody validation. One of the most common methods to achieve this is to immunoprecipitate (IP) the target with one antibody and subsequently detect it by western blotting with another antibody against the same target. This provides confidence that both antibodies are binding the correct biomolecule.
We’re honored to win two CiteAb awards in 2020: Antibody Supplier of the Decade and Antibody Supplier Succeeding in Alzheimer’s Research. CST was also highly commended as an ELISA Kit Supplier to Watch in 2020. It’s always nice to be recognized for your work. In this case, it’s especially sweet because both efforts are near and dear to our hearts.
Topics: Antibody Performance
An orthogonal strategy for antibody validation involves cross-referencing antibody-based results with data obtained using non-antibody-based methods. This approach is critical to verify existing antibody validation data and to identify any effects or artifacts that are directly related to the antibody in question. Providing an additional level of detail to support results generated by the other strategies outlined within this handbook, orthogonal validation often utilizes data which are available in the public domain.
In order to quickly defeat SARS-CoV-2 (severe acute respiratory syndrome-coronavirus-2，previously named as nCoV) and stop the epidemic, diagnosis and treatment are especially important. As of January 31, 2020, SARS-CoV-2 nucleic acid detection kits from 7 companies have been approved for SARS-CoV-2 detection (data source: NMPA). Meanwhile, researchers are still working hard to develop effective treatments.
While a binary approach is one of the more desirable ways to evaluate antibody specificity, binary models are not always readily available and can be time consuming or expensive to produce for the sole purpose of validating an antibody. Moreover, to assess the sensitivity of an antibody in the application and protocol being used, a complementary hallmark is required.
As we touched upon in a previous blog post, the innate immune system is an important part of our bodies’ immune defenses and it provides us with a general protective response against microbial invaders and foreign proteins. This innate immune system is evolutionarily older than our adaptive immune system, with conserved mechanisms seen in plants, animals, and insects, and it is not specialized against any particular pathogen.
We at Cell Signaling Technology (CST) have been paying close attention to news about the recent and rapid spread of a new form of coronavirus in China. As members of a global organization with many esteemed colleagues in China and all over the world, we are concerned for all who are affected by this terrible respiratory virus and are eager to provide aid to those in need.
Topics: Corporate Social Responsibility
A binary approach is one of the best ways to evaluate antibody specificity. By testing an antibody in biologically relevant positive and negative expression systems, it is possible to confirm that it recognizes the target antigen in its native environment without cross-reacting with other biomolecules present in the sample.