4 Steps to Better Immunohistochemistry: Steps 2, 3, and 4


Posted by Carolyn P on Aug 31, 2016 3:00:00 AM

This is part two of a two-part series on how to optimize your IHC protocols. Part one introduced the principles behind antigen retrival. Click here if you missed it...but, if you've got your tissue prepped and ready to go, we'll move on to the next steps in the staining protocol.

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Topics: Protocols, IHC, techniques

4 Steps to Better Immunohistochemistry: Step 1 - Antigen Retrieval


Posted by Carolyn P on Aug 24, 2016 3:00:00 AM

It’s Friday night and you could be out with your friends right now, but instead you’re tucked away in a dark little room filled with microscopes. Spending the evening in the lab seemed like a good choice at the time because you were certain this immunohistochemistry was going to reveal some small - but important - mystery of the universe to you.

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Topics: Protocols, IHC, Companion Reagents, techniques

Webinar | Targeting Cancer Pathways: Understanding Immune Checkpoints


Posted by Carolyn P on Aug 17, 2016 3:00:00 AM

 
In this webinar, the speakers will explore how tumors exploit immune modulatory mechanisms to generate and thrive in their own immunosuppressive microenvironment. In addition, they will:
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Topics: Cancer Immunology, Cancer Research

The Reproducibility Crisis: An open letter from CST's Chief Scientific Officer


Posted by Carolyn P on Aug 10, 2016 3:00:00 AM

 

A Message from our Chief Scientific Officer, Roberto Polakiewicz, Ph.D.

The time is now for the scientific community to come together to respond to the challenges of reproducibility and to be part of the solution . . . 

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Topics: Reproducibility

Reproducibility in Research Science - Join the Discussion.


Posted by Carolyn P on Aug 3, 2016 3:00:00 AM

Lack of reproducibility in scientific studies has been a major topic of conversation lately. It's an important conversation for us to have, given the potential of this problem to affect how the research community views the published literature.  Many potential reasons for the problem exist, but CST is focused on the role vendors play in ensuring the reagents being used by the scientific community are properly validated. 

These are important issues that need to be addressed by the scientific community and we should all be part of the conversation . . . 

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Topics: Reproducility

Webinar | Key Signaling Pathways in Cancer: Links to Developmental Biology


Posted by Carolyn P on Jul 27, 2016 3:00:00 AM

It is notable that the same pathways governing the cell growth, death, and differentiation decisions made during embryonic development are also common drivers of adult malignancy. In this webinar, we will explore the idea that a better understanding of developmental biology signaling pathways will advance our understanding of adult tumors and cancer stem cells as well as boost our ability to create effective therapeutics to fight a broad array of cancers.

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Topics: Developmental Biology, Webinars

Investing in our Communities and Promoting Science Education


Posted by Carolyn P on Jul 20, 2016 3:00:00 AM

If you want to build a ship, don't drum up the men to gather wood, divide the work and give orders. Instead, teach them to yearn for the vast and endless sea.
- Antoine de St. Exupery

Every generation has its dreamers; pioneering individuals who aren’t satisfied with the status quo, but who will drive forward towards something bigger, something better . . . 

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Topics: CSR

The genomic address of bivalent nucleosomes


Posted by Liana G on Jul 13, 2016 3:00:00 AM


INTRODUCTION:

The activity of genes and their regulatory elements is, in part, governed by their cell type-specific chromatin organization. Nucleosomes, the building blocks of chromatin, are wrapped around a core of histone proteins that are subject to many post-translational modifications that can either promote or silence gene expression.

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Topics: Epigenetics, Journal Club

Antibody Validation - for Reproducible Results


Posted by Carolyn P on Jul 6, 2016 3:30:00 AM

High-impact, peer-reviewed publications shape the thinking of their respective fields and impact the way investigators approach related research questions. Therefore, the suggestion that the data supporting some of these publications cannot be replicated is cause for concern.

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Topics: Primary Antibodies, Antibody Validation, Literature Review, Reproducibility

CRISPR: Science magazine’s 2015 breakthrough of the year


Posted by Liana G on Jun 29, 2016 3:30:00 AM

CRISPR, a nifty gene-editing tool, has swept the scientific community off its feet. It has a catchy name, it’s in every other publication, it is the subject of a volatile legal dispute involving claims to its intellectual property (1), its use has instigated a multitude of summits to do with its ethical ramifications and, despite the fact that it’s less than 4 years old, the Science journal named it the ‘breakthrough of the year’ (2). To give you some perspective, the other contenders were the New Horizon’s epic visit to the dwarf planet Pluto, the discovery of a lymphatic system in the brain, and the Ebola vaccine. 

Well, you get the picture, CRISPR is a big deal, but what on Earth is it and why are scientists so smitten by it?

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Topics: Literature Review, Reproducibility