STR Profiling: What's growing in your incubator?


Posted by Hayley R. on Mar 6, 2019 3:15:00 AM

Cell lines are a crucial part of life science research and development. But did you know an estimated 18-36% of cell lines are believed to be misidentified or cross-contaminated with another cell line?

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Topics: techniques, Reproducibility

Hallmarks of Cancer: Deregulating Cellular Energetics


Posted by Chris S on Feb 27, 2019 3:15:00 AM

Cancer cells need a lot of energy to grow fast—to do so, they show abnormal metabolic pathways.

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Topics: Cell Biology, Cancer Research

Water Wise in the Lab


Posted by Elias W on Feb 20, 2019 3:10:00 AM

It probably comes as no surprise to you, since you work in a lab, that labs use five times the amount of water as a standard office building.

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Topics: Corporate Social Responsibility, CSR, Sustainability

Studying Post-translationally Modified Sites and Disease Variants with PhosphoSitePlus®


Posted by Florian G. on Feb 13, 2019 3:10:00 AM

Post-translational modifications (PTMs) are employed in the cell to alter protein function, and typically involve small chemical changes on the surface of the protein. The dynamic enzyme-catalyzed process of adding or subtracting PTMs mediates signaling in the cell. In other words, PTMs enhance protein-protein communication, enabling the cell to respond to internal or external signals.

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Topics: Post Translational Modification

More Than a Phenotype: a Guide to Assessing Tumor-infiltrating Immune Cell Types and Functions


Posted by Emily A. on Feb 6, 2019 3:05:00 AM

Although the rise of cancer immunotherapy may seem meteoric to many, the history goes back over 100 years to William B. Coley, often referred to as the father of immunotherapy. 

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Topics: Immunology

What makes an antibody monoclonal, polyclonal, or recombinant?


Posted by Anthony C on Jan 30, 2019 3:10:00 AM

When you’re shopping for antibodies, there are so many factors to consider. For example, will it work in my cell or tissue model? Has it been tested in the application I want to use? Sometimes it’s a struggle to find what you need because your options are limited, but in other instances there may be several reagents that seem like they could work in your experiment.

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Topics: Antibody Performance, Primary Antibodies, Antibody Validation

Hallmarks of Cancer: Resisting Cell Death


Posted by Chris S on Jan 23, 2019 3:15:00 AM

One thing we know about cancer cells: they can resist death. They evade apoptosis, the mechanism that programs cell death once cells become damaged. Normally, apoptosis helps keep an organism healthy through growth and development, maintaining body tissue by removing infected or damaged cells. But cancer cells do not follow this process, no matter how abnormally they grow.

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Topics: Cancer Research

Can I accelerate my ELISA results?


Posted by Chris S on Jan 16, 2019 3:10:00 AM

Running an ELISA can be a pain. Identifying pairs for an ELISA is a tedious business, and that’s before developing and validating the ELISA assay itself. Using a kit can simplify the process, but at what cost? Will that kit hinder reproducibility by introducing lot-to-lot variability over the course of my project’s lifetime? Many kits still require numerous reagent addition, incubation, and wash steps that add hands-on time and complexity to your assay.

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Topics: ELISA, techniques

When should I use flow cytometry for signaling instead of western blot?


Posted by Chris S on Jan 9, 2019 3:15:00 AM

Flow cytometry enables quantitative analysis of protein expression, signaling states, and physical characteristics (cell size/granularity) at the single-cell level. Modern flow cytometers are capable of collecting data on multiple proteins from thousands of cells per second in a heterogeneous mixture. While flow cytometry is commonly employed to identify cell types using phenotypic markers expressed on the cell surface, it can also be used to measure intracellular signaling events.

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Topics: Flow, techniques

Reflecting on the New Year with Janus and Jak


Posted by Ken B on Jan 2, 2019 10:45:49 AM

In Roman mythology, the New Year and the month of January are associated with Janus, the god of transitions and doorways (1). Janus is best known for having two faces: one looking to the past, and one to the future. Janus also lends his name to the Janus kinase (Jak) family of nonreceptor tyrosine kinases.

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Topics: Just for fun, Immunology

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