CST BLOG: Lab Expectations

The official blog of Cell Signaling Technology (CST) where we discuss what to expect from your time at the bench, share tips, tricks, and information.

Lab Expectations listing page

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Strategies for Successful Multiplex Immunofluorescence Experiments Using Conjugated Antibodies

One of the benefits of working with immunofluorescent (IF) analysis is the ability to compare multip...
Ginny Bain, PhD Apr 6, 2022

Why Do We Love Conjugation? Ask Reggie!

Reggie Prioli, Associate Director of Conjugation at Cell Signaling Technology® (CST®), has been conj...
Andrea T Feb 14, 2022

Tools for Classifying Cells and Identifying Protein Localization

When do I want to classify cells? Why do I care where a protein localizes? These are actually two di...
Andrea T Jan 26, 2022

Subcellular Marker Selection for Immunofluorescence

When performing immunofluorescence (IF), it is common to multiplex with several antibodies and/or dy...
Ginny Bain, PhD Jan 12, 2022

Multiplexing the Brain: A Galaxy of Possibilities

The average human brain contains over 100 billion cells, which is nearly the same number as there ar...
Richard Cho, PhD Dec 15, 2021

Tools for Monitoring Cell State

Have you been wondering how to monitor a cell state or cellular response? From a basic research stan...
Andrea T Oct 6, 2021

How S6 Ribosomal Protein (5G10) Rabbit mAb Can Help You With Your Immunofluorescence Staining

One antibody is used in our lab so often that we each keep our own bulk stock! This antibody is S6 R...
John B Jan 27, 2021

Pros and Cons of Different Multiplexing Techniques for mIHC and mIF

Multiplex immunohistochemistry (mIHC) and multiplex immunofluorescence (mIF) are widely used to iden...
Emma E Jul 22, 2020

10 Must-have Markers for Senescence Research

Understanding when and why cell cycle arrest occurs is critical to many fields of research, includin...
Tamar A May 13, 2020

Fluorescent Staining Using Multiple Antibodies: Two Common Techniques

The use of multiple antibodies in a single experiment can provide useful information to researchers....
Tara W Sep 13, 2017

Successful Immunofluorescence: Antibody Dilution & Incubation Conditions

Part four of a four-part series on successful immunofluorescence. Read Part 1: The Importance of Val...
Kenneth Buck, PhD May 10, 2017

Successful Immunofluorescence: Fixation and Permeabilization

Part Three of a four-part series on successful immunofluorescence. Read Part 1: The Importance of Va...
Kenneth Buck, PhD Mar 15, 2017

Successful Immunofluorescence: Experimental Controls

Part two of a four-part series on successful immunofluorescence. Read Part 1: The Importance of Vali...
Kenneth Buck, PhD Mar 8, 2017

Successful Immunofluorescence: The Importance of Validation

Part 1 of a four-part series on successful immunofluorescence. Read Part 2: Experimental Controls, P...
Kenneth Buck, PhD Mar 1, 2017

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