CST BLOG: Lab Expectations

The official blog of Cell Signaling Technology (CST), where we discuss what to expect from your time at the bench, share tips, tricks, and information.

Lab Expectations listing page

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Validating a Monoclonal Antibody for the ADP-Ribosylation PTM

Protein adenosine diphosphate, or ADP-ribosylation, is a fascinating post-translational modification...

High-Throughput 3D Assay Methods: Analysis of TGF-β Signaling

Spheroids and related three-dimensional (3D) cell models are rapidly gaining popularity among resear...
Alexandra Foley Aug 16, 2023

Strategies for Successful Multiplex IF Experiments Using Conjugated Antibodies

One of the benefits of working with immunofluorescent (IF) analysis is the ability to compare multip...
Ginny Bain, PhD Apr 6, 2022

Subcellular Marker Selection for Immunofluorescence

When performing immunofluorescence (IF), it is common to multiplex with several antibodies and/or dy...
Ginny Bain, PhD Jan 12, 2022

Multiplexing the Brain: A Galaxy of Possibilities

The average human brain contains over 100 billion cells, which is nearly the same number as there ar...
Richard Cho, PhD Dec 15, 2021

How S6 Ribosomal Protein (5G10) Rabbit mAb Can Help You With Your Immunofluorescence Staining

One antibody is used in our lab so often that we each keep our own bulk stock! This antibody is S6 R...
John B Jan 27, 2021

Simplify iPSCs Research: Antibodies to Identify Pluripotency Markers

Reagent validation and experiment reproducibility are critical for advancing research in any scienti...
Chris Sumner Sep 30, 2020

Pros and Cons of Different Multiplexing Techniques for IHC and IF

Multiplex immunohistochemistry (mIHC) and multiplex immunofluorescence (mIF) are widely used to iden...
Alexandra Foley Jul 22, 2020

10 Must-have Markers for Senescence Research

Understanding when and why cell cycle arrest occurs is critical to many fields of research, includin...
Tamar Aprahamian, PhD May 13, 2020

Tissue Clearing from a Microscope Enthusiast’s Perspective

Biological imaging data has massive potential in terms of complexity of experiment and richness of d...
Ginny Bain, PhD Oct 30, 2019

Fluorescent Staining Using Multiple Antibodies: Two Common Techniques

The use of multiple antibodies in a single experiment can provide useful information to researchers....
Tara White Sep 13, 2017

Successful Immunofluorescence: Antibody Dilution & Incubation Conditions

Part four of a four-part series on successful immunofluorescence. Read Part 1: The Importance of Val...
Kenneth Buck, PhD May 10, 2017

Successful Immunofluorescence: Fixation and Permeabilization

Part Three of a four-part series on successful immunofluorescence. Read Part 1: The Importance of Va...
Kenneth Buck, PhD Mar 15, 2017

Successful Immunofluorescence: Experimental Controls

Part two of a four-part series on successful immunofluorescence. Read Part 1: The Importance of Vali...
Kenneth Buck, PhD Mar 8, 2017

Successful Immunofluorescence: The Importance of Validation

Part 1 of a four-part series on successful immunofluorescence. Read Part 2: Experimental Controls, P...
Kenneth Buck, PhD Mar 1, 2017

Immunofluorescence: The Questions to Ask Before Starting Your Next Experiment

Specificity, consistency, and optimized assay conditions are three key elements that help ensure rel...
Liana G Oct 19, 2016

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