CST BLOG: Lab Expectations

The official blog of Cell Signaling Technology (CST) where we discuss what to expect from your time at the bench, share tips, tricks, and information.

Lab Expectations listing page

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CST® Antibodies Validated on the Leica Microsystems Cell DIVE Multiplexed Imaging Solution

True innovation comes from collaboration–from bringing experts together to create techniques and too...
Alexandra Foley Mar 15, 2023

Increase Your Experimental Success with CUT&RUN-Validated Antibodies

CUT&RUN delivers the power of chromatin immunoprecipitation (ChIP) or ChIP-sequencing (ChIP-seq)...
Christopher Fry, PhD Oct 26, 2022

Tools to Confirm Antibody Specificity

You want to generate accurate results. You’ve planned out your experiment, accounting for all necess...
Chris Sumner Mar 2, 2022

The Importance of Using a Validated Antibody Pair in a High-throughput Immunoassay

Over the years, there have been numerous additions and variations to the original ELISA format inclu...
Mark S Sep 29, 2021

Protect Your Pipeline: Don't let antibody supply disrupt your progress

During the pandemic, supply shortages took many of us by surprise. Who would have thought items as d...
Josh N Jun 17, 2020

The Importance of Research Antibody Specificity and Reproducibility During the SARS-CoV-2 Crisis

During this time of crisis, healthcare providers and researchers around the world are racing to deve...

Hallmarks of Validation - Complementary Strategies

Depending on the antigenic target being studied and the application being used, it may be advisable ...
Katie Crosby Apr 8, 2020

Hallmarks of Antibody Validation: Heterologous Strategy

For antigenic targets where expression of the protein is very low or unknown, the use of recombinant...
Katie Crosby Apr 1, 2020

Hallmarks of Validation - Multiple Antibody Strategy

A multiple antibody strategy is a powerful approach to antibody validation. One of the most common m...
Katie Crosby Mar 18, 2020

Hallmarks of Validation - Orthogonal Strategy

An orthogonal strategy for antibody validation involves cross-referencing antibody-based results wit...
Katie Crosby Mar 4, 2020

Hallmarks of Validation - Ranged Strategy

While a binary approach is one of the more desirable ways to evaluate antibody specificity, binary m...
Katie Crosby Feb 19, 2020

Hallmarks of Antibody Validation - Binary Strategy

A binary approach is one of the best ways to evaluate antibody specificity. By testing an antibody i...
Katie Crosby Jan 22, 2020

Introduction to the Hallmarks of Antibody Validation

Antibodies are essential reagents that support all levels of scientific research. Used in a multitud...
Chris Sumner Jan 15, 2020

Behind the Antibodies - Interview with a Development Scientist

Have you ever wondered about the minds behind our antibodies? We talk a lot about validation, specif...
Chris Sumner Oct 16, 2019

What makes an antibody monoclonal, polyclonal, or recombinant?

When you’re shopping for antibodies, there are so many factors to consider. For example, will it wor...
Chris Sumner Jan 30, 2019

Antibody Validation 101

As a development scientist at CST, people sometimes ask me what exactly it is that I do. “I make ant...
Susan K Mar 7, 2018

Antibody Company of the Year (2018)

We as scientists learn from each success and failure. Sometimes it takes many failures to achieve su...
Chris Sumner Feb 7, 2018

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