CST BLOG: Lab Expectations

The official blog of Cell Signaling Technology® (CST) where we discuss what to expect from your time at the bench, share tips, tricks, and information.

Tools to Confirm Antibody Specificity

You want to generate accurate results. You’ve planned out your experiment, accounting for all necess...
Chris S Mar 2, 2022 3:00:00 AM

The Importance of Using a Validated Antibody Pair in a High-throughput Immunoassay

Over the years there have been numerous additions and variations to the original ELISA format includ...
Mark S Sep 29, 2021 3:00:00 AM

Protect Your Pipeline: Don't let antibody supply disrupt your progress

During the pandemic, supply shortages took many of us by surprise. Who would have thought items as d...
Josh N Jun 17, 2020 3:00:00 AM

The Importance of Research Antibody Specificity and Reproducibility During the SARS-CoV-2 Crisis

During this time of crisis, healthcare providers and researchers around the world are racing to deve...
Srikanth S May 6, 2020 3:38:00 PM

Hallmarks of Validation - Complementary Strategies

Depending on the antigenic target being studied and the application being used, it may be advisable ...
Katie C Apr 8, 2020 3:00:00 AM

Hallmarks of Validation - Heterologous Strategy

For antigenic targets where expression of the protein is very low or unknown, the use of recombinant...
Katie C Apr 1, 2020 3:00:00 AM

Hallmarks of Validation - Multiple Antibody Strategy

A multiple antibody strategy is a powerful approach to antibody validation. One of the most common m...
Katie C Mar 18, 2020 3:00:00 AM

Hallmarks of Validation - Orthogonal Strategy

An orthogonal strategy for antibody validation involves cross-referencing antibody-based results wit...
Katie C Mar 4, 2020 3:00:00 AM

Hallmarks of Validation - Ranged Strategy

While a binary approach is one of the more desirable ways to evaluate antibody specificity, binary m...
Katie C Feb 19, 2020 3:15:00 AM

Hallmarks of Antibody Validation - Binary Strategy

A binary approach is one of the best ways to evaluate antibody specificity. By testing an antibody i...
Katie C Jan 22, 2020 3:00:00 AM

Introduction to the Hallmarks of Antibody Validation

Antibodies are essential reagents that support all levels of scientific research. Used in a multitud...
Chris S Jan 15, 2020 3:00:00 AM

Behind the Antibodies - Interview with a Development Scientist

Have you ever wondered about the minds behind our antibodies? We talk a lot about validation, specif...
Chris S Oct 16, 2019 3:15:00 AM

What makes an antibody monoclonal, polyclonal, or recombinant?

When you’re shopping for antibodies, there are so many factors to consider. For example, will it wor...
Anthony C Jan 30, 2019 3:10:00 AM

Myth-busting for ChIP/ChIP-seq: Mono vs. Poly

Researchers who run a lot of chromatin immunoprecipitation "ChIP" assays – maybe even your advisor –...
Neha G and Ken B Sep 26, 2018 3:15:00 AM

Antibody Validation 101

As a development scientist at CST, people sometimes ask me what exactly it is that I do. “I make ant...
Susan K Mar 7, 2018 6:15:00 AM

Antibody Company of the Year (2018)

We as scientists learn from each success and failure. Sometimes it takes many failures to achieve su...
Chris S Feb 7, 2018 6:15:00 AM

Webinar | Achieving Reproducibility: Don't Let Antibodies Be a Variable in Your Experiment

The importance of antibodies as tools in scientific research studies cannot be understated, yet thes...
Chris S Jan 10, 2018 6:30:00 AM

Switching from Manual to Automated IHC? We've got your back

If you’ve ever transitioned your IHC experiments from a manual protocol to an automated platform, yo...
Chris G Aug 30, 2017 3:00:00 AM
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