Hallmarks of Antibody Validation - Binary Strategy


Posted by Katie C on Jan 22, 2020 3:00:00 AM

A binary approach is one of the best ways to evaluate antibody specificity. By testing an antibody in biologically relevant positive and negative expression systems, it is possible to confirm that it  recognizes the target antigen in its native environment without cross-reacting with other biomolecules present in the sample.

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Topics: Antibody Performance, Antibody Validation

Introduction to the Hallmarks of Antibody Validation


Posted by Chris S on Jan 15, 2020 3:00:00 AM

Antibodies are essential reagents that support all levels of scientific research. Used in a multitude of applications to identify, quantify, and isolate specific target biomolecules, they have recently become the focus of intense scrutiny for their contribution to the ongoing reproducibility crisis.

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Topics: Antibody Performance, Antibody Validation

Behind the Antibodies - Interview with a Development Scientist


Posted by Chris S on Oct 16, 2019 3:15:00 AM

Have you ever wondered about the minds behind our antibodies? We talk a lot about validation, specificity, sensitivity, and reproducibility. All of that is very important, but that doesn't tell you much about who developed it.

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Topics: Antibody Performance, Primary Antibodies, Antibody Validation

What makes an antibody monoclonal, polyclonal, or recombinant?


Posted by Anthony C on Jan 30, 2019 3:10:00 AM

When you’re shopping for antibodies, there are so many factors to consider. For example, will it work in my cell or tissue model? Has it been tested in the application I want to use? Sometimes it’s a struggle to find what you need because your options are limited, but in other instances there may be several reagents that seem like they could work in your experiment.

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Topics: Antibody Performance, Primary Antibodies, Antibody Validation

Myth-busting for ChIP/ChIP-seq: Mono vs. Poly


Posted by Neha G and Ken B on Sep 26, 2018 3:15:00 AM

Researchers who run a lot of chromatin immunoprecipitation "ChIP" assays – maybe even your advisor – might subscribe to the idea that polyclonal antibodies perform better than monoclonal antibodies. But is that always actually true?

It’s worth your time to understand the differences between the two in terms of antigen recognition and specificity, and dispel some myths. 

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Topics: Antibody Performance, ChIP, Antibody Validation, Post Translational Modification, Reproducibility

Antibody Validation 101


Posted by Susan K on Mar 7, 2018 6:15:00 AM

As a development scientist at CST, people sometimes ask me what exactly it is that I do. “I make antibodies,” is my typical reply, but that is not exactly true.

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Topics: Antibody Validation

Antibody Company of the Year (2018)


Posted by Chris S on Feb 7, 2018 6:15:00 AM

We as scientists learn from each success and failure. Sometimes it takes many failures to achieve success. And some discoveries are made with no fanfare, far from the spotlight. Other times, a good day’s work is even sweeter when you realize someone noticed!

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Topics: Antibody Performance, Antibody Validation, Cancer Research, Reproducility

Webinar | Achieving Reproducibility: Don't Let Antibodies Be a Variable in Your Experiment


Posted by Chris S on Jan 10, 2018 6:30:00 AM

The importance of antibodies as tools in scientific research studies cannot be understated, yet these reagents have increasingly come under fire for their lack of reproducibility. Part of the issue is that the antibody market is composed of hundreds of vendors and resellers with varying definitions for validation and consistency. Cell Signaling Technology (CST) believes that antibody suppliers should be held accountable for the products they provide, but that vendors alone cannot solve the reproducibility “crisis." How antibodies are validated and used in the laboratory is a critical component to this process. Researchers need to be more attentive to following established protocols and leverage the expertise of the scientists who have developed and tested the product they are using. Journals need to be more active in enforcing existing policies regarding materials and methods or develop more clear-cut means to identify and describe the use of biological reagents in published research. During this webinar we will address the role vendors, researchers, and journals should play in minimizing irreproducibility. We will also outline CST’s antibody validation process, while highlighting steps all users should consider when selecting and using antibodies in their research.

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Topics: Antibody Performance, Antibody Validation, Webinars, Reproducibility

5 Steps to Publication-Worthy IF Images


Posted by Tara W on Sep 6, 2017 3:30:00 PM

A picture is worth a thousand words, or in the case of immunofluorescent imaging, a thousand proteins. The images used to illustrate a scientific experiment should convey as much information as the text itself. Here at CST, we pride ourselves in the quality of our antibodies and our rigorous validation process. When we approve our primary antibodies for IF, we like to showcase them using high quality images generated in-house. Beyond our recommended IF protocols (check it out here), here are some additional considerations to make when planning your IF staining. 

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Topics: Protocols, Antibody Performance, Antibody Validation, IF-IC, techniques

Switching from Manual to Automated IHC? We've got your back


Posted by Chris G on Aug 30, 2017 3:00:00 AM

If you’ve ever transitioned your IHC experiments from a manual protocol to an automated platform, you may have found the conversion process to be a drag. It isn’t an easy thing to do. For that reason, we’re happy to announce our IHC Leadership in Automation initiative. This rigorous validation initiative expands on our already thorough measures, allowing researchers to not only use CST products with our recommended manual IHC protocol, but also to bridge the assay to new platforms and techniques. Our foray into the world of automated IHC aims to reduce the amount of time researchers spend on assay transfer and protocol optimization.

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Topics: Protocols, IHC, Antibody Performance, Antibody Validation, techniques, Automated IHC

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