CST BLOG: Lab Expectations

The official blog of Cell Signaling Technology® (CST) where we discuss what to expect from your time at the bench, share tips, tricks, and information.

Protect Your Pipeline: Don't let antibody supply disrupt your progress

During the pandemic, supply shortages took many of us by surprise. Who would have thought items as d...
Josh N Jun 17, 2020 3:00:00 AM

The Importance of Research Antibody Specificity and Reproducibility During the SARS-CoV-2 Crisis

During this time of crisis, healthcare providers and researchers around the world are racing to deve...
Srikanth S May 6, 2020 3:38:00 PM

Hallmarks of Validation - Complementary Strategies

Depending on the antigenic target being studied and the application being used, it may be advisable ...
Katie C Apr 8, 2020 3:00:00 AM

Hallmarks of Validation - Heterologous Strategy

For antigenic targets where expression of the protein is very low or unknown, the use of recombinant...
Katie C Apr 1, 2020 3:00:00 AM

Hallmarks of Validation - Multiple Antibody Strategy

A multiple antibody strategy is a powerful approach to antibody validation. One of the most common m...
Katie C Mar 18, 2020 3:00:00 AM

Hallmarks of Validation - Orthogonal Strategy

An orthogonal strategy for antibody validation involves cross-referencing antibody-based results wit...
Katie C Mar 4, 2020 3:00:00 AM

Hallmarks of Validation - Ranged Strategy

While a binary approach is one of the more desirable ways to evaluate antibody specificity, binary m...
Katie C Feb 19, 2020 3:15:00 AM

Hallmarks of Antibody Validation - Binary Strategy

A binary approach is one of the best ways to evaluate antibody specificity. By testing an antibody i...
Katie C Jan 22, 2020 3:00:00 AM

Introduction to the Hallmarks of Antibody Validation

Antibodies are essential reagents that support all levels of scientific research. Used in a multitud...
Chris S Jan 15, 2020 3:00:00 AM

Behind the Antibodies - Interview with a Development Scientist

Have you ever wondered about the minds behind our antibodies? We talk a lot about validation, specif...
Chris S Oct 16, 2019 3:15:00 AM

What makes an antibody monoclonal, polyclonal, or recombinant?

When you’re shopping for antibodies, there are so many factors to consider. For example, will it wor...
Anthony C Jan 30, 2019 3:10:00 AM

Myth-busting for ChIP/ChIP-seq: Mono vs. Poly

Researchers who run a lot of chromatin immunoprecipitation "ChIP" assays – maybe even your advisor –...
Neha G and Ken B Sep 26, 2018 3:15:00 AM

Antibody Validation 101

As a development scientist at CST, people sometimes ask me what exactly it is that I do. “I make ant...
Susan K Mar 7, 2018 6:15:00 AM

Antibody Company of the Year (2018)

We as scientists learn from each success and failure. Sometimes it takes many failures to achieve su...
Chris S Feb 7, 2018 6:15:00 AM

Webinar | Achieving Reproducibility: Don't Let Antibodies Be a Variable in Your Experiment

The importance of antibodies as tools in scientific research studies cannot be understated, yet thes...
Chris S Jan 10, 2018 6:30:00 AM

Switching from Manual to Automated IHC? We've got your back

If you’ve ever transitioned your IHC experiments from a manual protocol to an automated platform, yo...
Chris G Aug 30, 2017 3:00:00 AM

Successful Immunofluorescence: Experimental Controls

 Part two of a four-part series on Immunofluorescence. Check out our posts on Validation and Fixatio...
Ken B Mar 8, 2017 3:00:00 AM

Successful Immunofluorescence: The Importance of Validation

Part one of a four-part series on Immunofluorescence. Check out Experimental Controls and Fixation a...
Ken B Mar 1, 2017 3:00:00 AM

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