Running an ELISA can be a pain. Identifying pairs for an ELISA is a tedious business, and that’s before developing and validating the ELISA assay itself. Using a kit can simplify the process, but at what cost? Will that kit hinder reproducibility by introducing lot-to-lot variability over the course of my project’s lifetime? Many kits still require numerous reagent addition, incubation, and wash steps that add hands-on time and complexity to your assay.