Hallmarks of Cancer: Sustaining Proliferative Signaling


Posted by Chris S on Apr 3, 2019 3:15:00 AM

Cancer cells stimulate their own growth, which means they become self-sufficient in growth signals, and no longer depend on external signals (like Epidermal Growth Factor EGF/ EGFR). Proliferation depends highly on these three important pathways: Akt, MAPK/Erk, and MTOR.

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Topics: Cell Biology, Cancer Research

Hallmarks of Cancer: Inducing Angiogenesis Energetics


Posted by Chris S on Mar 20, 2019 3:15:00 AM

Cancer cells stimulate the growth of blood vessels to supply nutrients to tumors. Angiogenesis is the formation of new blood vessels from pre-existing blood vessels. This plays an important role in tumor growth.

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Topics: Cell Biology, Cancer Research

Hallmarks of Cancer: Deregulating Cellular Energetics


Posted by Chris S on Feb 27, 2019 3:15:00 AM

Cancer cells need a lot of energy to grow fast—to do so, they show abnormal metabolic pathways.

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Topics: Cell Biology, Cancer Research

Hallmarks of Cancer: Resisting Cell Death


Posted by Chris S on Jan 23, 2019 3:15:00 AM

One thing we know about cancer cells: they can resist death. They evade apoptosis, the mechanism that programs cell death once cells become damaged. Normally, apoptosis helps keep an organism healthy through growth and development, maintaining body tissue by removing infected or damaged cells. But cancer cells do not follow this process, no matter how abnormally they grow.

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Topics: Cancer Research

Can I accelerate my ELISA results?


Posted by Chris S on Jan 16, 2019 3:10:00 AM

Running an ELISA can be a pain. Identifying pairs for an ELISA is a tedious business, and that’s before developing and validating the ELISA assay itself. Using a kit can simplify the process, but at what cost? Will that kit hinder reproducibility by introducing lot-to-lot variability over the course of my project’s lifetime? Many kits still require numerous reagent addition, incubation, and wash steps that add hands-on time and complexity to your assay.

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Topics: ELISA, techniques

When should I use flow cytometry for signaling instead of western blot?


Posted by Chris S on Jan 9, 2019 3:15:00 AM

Flow cytometry enables quantitative analysis of protein expression, signaling states, and physical characteristics (cell size/granularity) at the single-cell level. Modern flow cytometers are capable of collecting data on multiple proteins from thousands of cells per second in a heterogeneous mixture. While flow cytometry is commonly employed to identify cell types using phenotypic markers expressed on the cell surface, it can also be used to measure intracellular signaling events.

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Topics: Flow, techniques

Webinar | Deciphering Neurodegeneration: Inflammation, immune response, and Alzheimer's


Posted by Chris S on Nov 14, 2018 3:15:00 AM

Hear about the potential role of innate immunity in neurodegeneration and cognitive function, particularly in Alzheimer's disease. Learn how immune-related pathways regulate the development, refinement, and elimination of specific axons and synapses during development. Gain an understanding of how recent work can provide insight into protecting synapses in neurodegenerative and psychiatric disorders of synaptic dysfunction.

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Topics: Webinars

Mastering Phospho-Flow: Immune Cell Activation Webinar


Posted by Chris S on Jul 11, 2018 3:15:00 AM

To characterize activation of immune cells, measurement of intracellular signaling using phospho-specific antibodies combined with immune phenotyping using surface markers is ideal. Owing to the unique capability of flow cytometry to measure events at a single-cell level, “Phospho Flow” allows for the identification of rare signaling events during processes such as co-stimulatory signaling and manipulation of checkpoint inhibition. However, combining extracellular and intracellular antibodies in a flow protocol can present technical challenges. 
 
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Topics: Protocols, Flow

Patriotic Lab Bench


Posted by Chris S on Jul 4, 2018 3:15:00 AM

Our headquarters happens to be in the U.S. So we're taking some time to celebrate the Fourth of July. But also, Sunday was Canada Day for our northern neighbors and Bastille Day is coming up on July 14th. Happy Independence Day to anyone celebrating around the world. And to Australia, Cheers Mate, Happy Winter.

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Topics: Just for fun

Painless Publication: How to Write a Conference Abstract


Posted by Chris S on Jun 27, 2018 3:15:00 AM

Our previous blog post, “Painless Publication: How to Write a Journal Abstract,” walked you through the steps in writing the abstract for a journal article. Now we turn our focus to writing abstracts for conference proceedings. Although there are some similarities between these two types of abstracts, there are also some distinct considerations and approaches for conference abstracts.

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Topics: techniques, Q&A, Career Development, Science Education

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