CST BLOG: Lab Expectations

The official blog of Cell Signaling Technology (CST), where we discuss what to expect from your time at the bench, share tips, tricks, and information.

Shipping Temperature and Antibody Performance: You Asked. CST Answers.

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Here at CST, we frequently get questions about the proper shipping temperature for antibodies. So we thought we would do an interview with an expert on this topic, our very own Seneca Stone, who directs our global supply chain operations.

CST antibodies undergo rigorous stability testing to ensure qualityCST antibodies undergo rigorous stability testing to ensure quality. To reduce our carbon footprint, cold shipping is used only when necessary.


Question: Why do most of my CST orders ship at room temperature?

Seneca: We pride ourselves on top-quality antibodies, and stability testing is a key component to guaranteeing high quality. Many of our antibodies are unaffected by ambient temperatures during the short shipping period. In fact, roughly 60% of our antibodies are able to be shipped without any cooling measures needed. Only about 39% of our antibodies require cold shipping, and less than 1% require sub-zero shipping conditions.


Question: How is an antibody’s best shipping temperature determined?

Seneca: Each antibody undergoes testing to determine the impact of exposure to different temperatures over a one-week period. Samples of the antibody are packaged and stored at three temperatures: −20°C, room temperature, and 37°C. At the end of seven days, the antibodies are assessed to determine if there have been changes in their activity at any of the tested temperatures.

For example, the shipping temperature test results for our Anti-rabbit IgG HRP-linked Antibody #7074 demonstrate the consistent performance of the antibody after storage at all three temperatures:

Western blot analysis of extracts from SK-MEL-28 cells at 20C,  RT and 37C

Western blot analysis of extracts from SK-MEL-28 cells probed with EEA1 Antibody #2411 and GAPDH (D16H11) XP® Rabbit mAb #5174. The secondary, Anti-rabbit IgG HRP-linked Antibody #7074, was incubated at -20°C,  room temperature (RT), or 37°C for one week before being used for detection. 


If you receive an antibody at room temperature, rest assured that it has been thoroughly tested to ensure antibody performance is still optimal following shipping.


Question: What about ambient shipping during hot summer weather?

Seneca: It is possible that a shipment could be exposed to high temperatures as it sits inside the delivery truck on the day of delivery. That is why our test includes a temperature of 37°C (98.6°F). As shown in the results for Anti-rabbit IgG HRP-linked Antibody #7074, the antibody is stable even after one week at this elevated temperature, which far exceeds the length of time a shipment might be exposed to hot temperatures.

You can see more test results for other antibodies by visiting the CST Stability Test Results webpage.


Question: Why does the temperature vary from shipment to shipment?

Seneca: The shipping temperature for an order is determined based on the mix of products included in the order. If all items in an order have been verified stable at ambient temperature, then the entire order is shipped at ambient temperature. However, if one or more items require cold shipping, then the entire order is shipped in packaging designed to keep your product between 2 and 8°C until it arrives in your lab. If you typically receive your orders at ambient temperature but notice this changes when you include a new item in your usual order, it means the new item requires cold shipping.


Question: Does this mean I can store my antibodies at room temperature?

Seneca: In the majority of cases, long-term storage at ambient temperature is discouraged because it could result in antibody degradation. You should check the datasheet for each antibody you receive to determine the proper storage temperature. In most cases, your storage temperature will be −20°C, although some conjugated antibodies may be stored at 4°C. The correct storage temperature is listed on the vial.

Data sheets for each CST antibody are available on our Data Sheets webpage so you can easily identify or double-check the proper storage temperature for your antibodies.


Question: Why not just ship them all cold?

Seneca: Here at CST, we are always looking for viable ways of reducing our environmental footprint. By testing our antibodies for shipping temperature options, we are able to reduce the amount of shipping material waste generated while still maintaining the highest product quality.

We hope this information helps you understand the reasoning behind our shipping temperature strategy. We are here to answer any other questions you may have on this or other topics. Just reach out to our Technical Support team or call your CST representative. We are always happy to help!


View Stability Test Results

Chris Sumner
Chris Sumner
Chris Sumner was the Editor-in-Chief of Lab Expectations. When he's not reading/writing about curing disease, he's hiking in the woods, playing guitar, or searching for the world's best lobster roll.

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