Can I accelerate my ELISA results?


Posted by Chris S on Jan 16, 2019 3:10:00 AM

Running an ELISA can be a pain. Identifying pairs for an ELISA is a tedious business, and that’s before developing and validating the ELISA assay itself. Using a kit can simplify the process, but at what cost? Will that kit hinder reproducibility by introducing lot-to-lot variability over the course of my project’s lifetime? Many kits still require numerous reagent addition, incubation, and wash steps that add hands-on time and complexity to your assay.

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Topics: ELISA, techniques

When should I use flow cytometry for signaling instead of western blot?


Posted by Chris S on Jan 9, 2019 3:15:00 AM

Flow cytometry enables quantitative analysis of protein expression, signaling states, and physical characteristics (cell size/granularity) at the single-cell level. Modern flow cytometers are capable of collecting data on multiple proteins from thousands of cells per second in a heterogeneous mixture. While flow cytometry is commonly employed to identify cell types using phenotypic markers expressed on the cell surface, it can also be used to measure intracellular signaling events.

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Topics: Flow, techniques

Webinar | Deciphering Neurodegeneration: Inflammation, immune response, and Alzheimer's


Posted by Chris S on Nov 14, 2018 3:15:00 AM

Hear about the potential role of innate immunity in neurodegeneration and cognitive function, particularly in Alzheimer's disease. Learn how immune-related pathways regulate the development, refinement, and elimination of specific axons and synapses during development. Gain an understanding of how recent work can provide insight into protecting synapses in neurodegenerative and psychiatric disorders of synaptic dysfunction.

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Topics: Webinars

Mastering Phospho-Flow: Immune Cell Activation Webinar


Posted by Chris S on Jul 11, 2018 3:15:00 AM

To characterize activation of immune cells, measurement of intracellular signaling using phospho-specific antibodies combined with immune phenotyping using surface markers is ideal. Owing to the unique capability of flow cytometry to measure events at a single-cell level, “Phospho Flow” allows for the identification of rare signaling events during processes such as co-stimulatory signaling and manipulation of checkpoint inhibition. However, combining extracellular and intracellular antibodies in a flow protocol can present technical challenges. 
 
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Topics: Protocols, Flow

Painless Publication: How to Write a Conference Abstract


Posted by Chris S on Jun 27, 2018 3:15:00 AM

Our previous blog post, “Painless Publication: How to Write a Journal Abstract,” walked you through the steps in writing the abstract for a journal article. Now we turn our focus to writing abstracts for conference proceedings. Although there are some similarities between these two types of abstracts, there are also some distinct considerations and approaches for conference abstracts.

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Topics: techniques, Q&A, Career Development, Science Education

Responsible Science Podcast: Episode 5 - The Next Generation


Posted by Chris S on Jun 20, 2018 3:10:00 AM

We're excited to partner with Science: Disrupt to present a five-part podcast exploring what 'Responsible Science' means amidst the reproducibility crisis.

For the final episode of our ‘Responsible Science’ series, we look forward with some of the most exciting innovators coming up through the ranks of academia, intent on making it a better and more responsible place as they go.

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Topics: Reproducibility

Responsible Science Podcast: Episode 4 - Publishing


Posted by Chris S on Jun 13, 2018 3:20:00 AM

We're excited to partner with Science: Disrupt to present a five-part podcast exploring what 'Responsible Science' means amidst the reproducibility crisis.

For the fourth episode of our ‘Responsible Science’, we dive deep into the world of the science dissemination industry – organizations whose role is to effectively communicate science to the wider world, in the most efficient, fair and considered way possible, and ensure science can keep moving forward.

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Topics: Reproducibility, Science Education

Responsible Science Podcast: Episode 3 - The Industry


Posted by Chris S on Jun 6, 2018 3:10:00 AM

We're excited to partner with Science: Disrupt to present a five-part podcast exploring what 'Responsible Science' means amidst the reproducibility crisis.

For the third episode of our ‘Responsible Science’, we dive deep into the world of scientific industry – companies that bring science out of the lab, into the real world, and turn a profit at the same time.

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Topics: Reproducibility, Science Education

Responsible Science Podcast: Episode 2 - The Disruptors


Posted by Chris S on May 23, 2018 3:10:00 AM

We're excited to partner with Science: Disrupt to present a five-part podcast exploring what 'Responsible Science' means amidst the reproducibility crisis.

For the second episode of our ‘Responsible Science’ series, we dive deep into the world of startups intent on making science more responsible through new technologies, modern mentalities, and brand new tools.

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Topics: Reproducibility, Science Education

Shipping Temperature and Antibody Performance: You Asked. CST Answers.


Posted by Chris S on May 16, 2018 3:15:00 AM

Here at CST, we frequently get questions about the proper shipping temperature for antibodies. So we thought we would do an interview with an expert on this topic, our very own Seneca Stone who directs our global supply chain operations.

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Topics: Antibody Performance, Q&A

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