CST BLOG: Lab Expectations

The official blog of Cell Signaling Technology (CST) where we discuss what to expect from your time at the bench, share tips, tricks, and information.

Hallmarks of Cancer: Activation Invasion and Metastasis

Read More
All Posts

Cancer cells invade local tissue and spread to distant sites via two distinct, but similar processes known as invasion and metastasis.


Tissue invasion is the mechanism by which tumor cells expand into nearby environments. Metastasis refers to the process of tumor cells breaking away from the primary tumor, migrating to a new location and establishing a new, or secondary tumor, in the new environment. Both of these complex processes leverage existing cellular mechanisms, such as the Adherens Junction Signaling pathway, to enable invasion and migration of the tumor cells.

Adherens junctions are dynamic structures that form, strengthen and spread, degrade, and then re-form as their associated proteins create connections with counterparts from adjacent cells. The Adherens junction serves multiple functions, such as initiation and stabilization of cell-cell adhesion, regulation of the actin cytoskeleton, intracellular signaling, and transcriptional regulation. They can appear as bands encircling the cell (zonula adherens) or as spots of attachment to the extracellular matrix (adhesion plaques).

Under normal conditions, this helps create order among the cells. Cell-cell junctions link cells to adjacent cells in tissue. They also regulate tissue homeostasis in critical cell processes that include tissue barrier function, cell proliferation, and migration. Defects in cell-cell junctions give rise to a wide range of tissue abnormalities that disrupt homeostasis and are common in genetic abnormalities and cancers. 

The connection between cell junctions and the cytoskeleton is still under investigation. It may be more dynamic than originally considered and may rely on multiple, weak associations between the cadherin-catenin complex and the actin cytoskeleton or rely on other membrane-associated proteins.

Learn more about Adherens Junction Signaling and the proteins involved.

Checkout the complete guide to the Hallmarks of Cancer Research targets. Download the eBook Now.


1) Hanahan D, Weinberg RA (January 2000). "The Hallmarks of Cancer". Cell. 100 (1): 57–70. doi:10.1016/S0092-8674(00)81683-9
2) Hanahan D, Weinberg RA (March 2011). "Hallmarks of Cancer: the next generation". Cell. 144 (5):646-74. doi: 10.1016/j.cell.2011.02.013.
3) Garcia, Miguel A. (April 2018). “Cell-Cell Junctions Organize Structural and Signaling Networks to Regulate Epithelial Tissue Homeostasis”. Cold Spring Harb Perspect Biol. 2; 10(4): a029181.
4) Hartstock A, Nelson WJ (March 2008). “Adherens and Tight Junctions: Structure, Function and Connections to the Actin Cytoskeleton”. Biochim Biophys Acta. 1778(3): 660–669.

Chris Sumner
Chris Sumner
Chris Sumner was the Editor-in-Chief of Lab Expectations. When he's not reading/writing about curing disease, he's hiking in the woods, playing guitar, or searching for the world's best lobster roll.

Related Posts

SignalStar™ Multiplex IHC: Speed Your Spatial Biology Research with Ready-to-Use, 8-Plex Antibody Panels

Enabled by recent advances in sequencing, mass spectrometry, and fluorescence microscopy, spatial biology...
Sarah Klein, PhD Sep 12, 2023

Seeding Labs: Donated CST Lab Equipment Finds New Purpose in Malawi & Beyond

Researchers at the Malawi University of Science and Technology (MUST) exist in a constant state of emerge...
Alexandra Foley Sep 6, 2023