CST BLOG: Lab Expectations

The official blog of Cell Signaling Technology® (CST) where we discuss what to expect from your time at the bench, share tips, tricks, and information.

Which fluorophores can I combine in my flow cytometry experiment?

Flow cytometry offers the exciting ability to detect and quantify multiple readouts per cell, enabli...
Rob M Apr 13, 2022 3:00:00 AM

Strategies for Successful Multiplex Immunofluorescence Experiments Using Conjugated Antibodies

One of the benefits of working with immunofluorescent (IF) analysis is the ability to compare multip...
Ginny B. Apr 6, 2022 3:00:00 AM

Antibody Staining Guide for Flow Cytometry

Getting started with flow cytometry or beginning to branch out from working solely with live cells t...
Rob M Mar 23, 2022 3:00:00 AM

Subcellular Marker Selection for Immunofluorescence

When performing immunofluorescence (IF), it is common to multiplex with several antibodies and/or dy...
Ginny B. Jan 12, 2022 3:00:00 AM

Multiplexing the Brain: A Galaxy of Possibilities

The average human brain contains over 100 billion cells, which is nearly the same number as there ar...
Richard C Dec 15, 2021 3:00:00 AM

Using SignalScan to Investigate Infection and Immune Response

The highly contagious severe acute respiratory syndrome coronavirus-2 or SARS-CoV-2 is the cause of ...
Charles F Sep 22, 2021 3:00:00 AM

A Tale of Two Stains: Ponceau S vs Coomassie Blue

You've just finished running your lysate sample by SDS-PAGE, so you're pretty confident your protein...
Caroline J and Misbah K Dec 9, 2020 3:00:00 AM

Recommended Best Practices for Lysate Handling

The preparation of a cell lysate is crucial to the success of many assays, including techniques that...
Michelle G Sep 2, 2020 3:00:00 AM

4 Situations Where Flow Cytometry Might Be More Useful Than Western Blotting

Western blotting is used to detect proteins and analyze their expression using antibodies, but it do...
Chris S Dec 4, 2019 3:00:00 AM

Video: How to Choose Enzymatic or Sonication Protocol for ChIP

The success of your chromatin immunoprecipitation (ChIP) experiment depends on the fragmentation of ...
Ken B Nov 6, 2019 3:00:00 AM

Imaging Mass Cytometry: Endless possibilities to understand the microenvironment of disease

Understanding the microenvironment of disease is critical. Targeted cancer therapies may not be effe...
Kris H Oct 23, 2019 3:15:00 AM

General Tips for Successful Cell Culture

Culturing cells in the lab? Following these tips will add to your success and help you avoid wasted ...
Dan D Apr 24, 2019 3:10:00 AM

STR Profiling: What's growing in your incubator?

Cell lines are a crucial part of life science research and development. But did you know an estimate...
Hayley R. Mar 6, 2019 3:15:00 AM

Can I accelerate my ELISA results?

Running an ELISA can be a pain. Identifying pairs for an ELISA is a tedious business, and that’s bef...
Chris S Jan 16, 2019 3:10:00 AM

When should I use flow cytometry for signaling instead of western blot?

Flow cytometry enables quantitative analysis of protein expression, signaling states, and physical c...
Chris S Jan 9, 2019 3:15:00 AM

Mycoplasma Contamination!

Any form of cell culture contamination can ruin your day and destroy your hard work, but mycoplasma ...
Michelle G Dec 5, 2018 3:15:00 AM

Writing a Grant Part 4: Additional Details

Finally, the finish line is approaching! You have completed your specific aims, significance and inn...
Tamar A. Nov 7, 2018 3:10:00 AM

Writing a Grant Part 3: The Experimental Approach

In the previous post, we described how to write an effective significance and innovation section, fo...
Tamar A. Oct 31, 2018 3:10:00 AM
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