CST BLOG: Lab Expectations

The official blog of Cell Signaling Technology® (CST) where we discuss what to expect from your time at the bench, share tips, tricks, and information.

Lab Expectations listing page

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Which Fluorophores Can I Combine in My Flow Cytometry Experiment?

Flow cytometry offers the exciting ability to detect and quantify multiple readouts per cell, enabli...
Rob MacDonald, PhD Apr 13, 2022

Antibody Staining Guide for Flow Cytometry

Getting started with flow cytometry or beginning to branch out from working solely with live cells t...
Rob MacDonald, PhD Mar 23, 2022

Subcellular Marker Selection for Immunofluorescence

When performing immunofluorescence (IF), it is common to multiplex with several antibodies and/or dy...
Ginny Bain, PhD Jan 12, 2022

Multiplexing the Brain: A Galaxy of Possibilities

The average human brain contains over 100 billion cells, which is nearly the same number as there ar...
Richard Cho, PhD Dec 15, 2021

Using SignalScan to Investigate Infection and Immune Response

The highly contagious severe acute respiratory syndrome coronavirus-2 or SARS-CoV-2 is the cause of ...

A Tale of Two Stains: Ponceau S vs. Coomassie Blue

You've just finished running your lysate sample by SDS-PAGE, so you're pretty confident your protein...

Recommended Best Practices for Lysate Handling

The preparation of a cell lysate is crucial to the success of many assays, including techniques that...
Michelle G Sep 2, 2020

4 Situations Where Flow Cytometry Might Be More Useful Than Western Blotting

Western blotting is used to detect proteins and analyze their expression using antibodies, but it do...
Chris Sumner Dec 4, 2019

Video: How to Choose Enzymatic or Sonication Protocol for ChIP

The success of your chromatin immunoprecipitation (ChIP) experiment depends on the fragmentation of ...
Kenneth Buck, PhD Nov 6, 2019

General Tips for Successful Cell Culture

Culturing cells in the lab? Following these tips will add to your success and help you avoid wasted ...
Dan D Apr 24, 2019

STR Profiling: What's Growing in Your Incubator?

Cell lines are a crucial part of life science research and development. But did you know an estimate...
Hayley R Mar 6, 2019

Can I accelerate my ELISA results?

Running an ELISA can be a pain. Identifying pairs for an ELISA is a tedious business, and that’s bef...
Chris Sumner Jan 16, 2019

When should I use flow cytometry for signaling instead of western blot?

Flow cytometry enables quantitative analysis of protein expression, signaling states, and physical c...
Chris Sumner Jan 9, 2019

Mycoplasma Contamination!

Any form of cell culture contamination can ruin your day and destroy your hard work, but mycoplasma ...
Michelle G Dec 5, 2018

Writing a Grant Part 4: Additional Details

Finally, the finish line is approaching! You have completed your specific aims, significance and inn...
Tamar A Nov 7, 2018

Writing a Grant Part 3: The Experimental Approach

In the previous post, we described how to write an effective significance and innovation section, fo...
Tamar A Oct 31, 2018

Writing a Grant Part 2: Significance and Innovation

The significance and innovation section is a recent (within the last 10 years) addition to the NIH a...
Tamar A Oct 24, 2018

Writing a Grant Part 1: First Things First

So you’re thinking of writing a grant? Or maybe your mentor has politely suggested that it would be ...
Tamar A Oct 17, 2018

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