4 Situations Where Flow Cytometry Might Be More Useful Than Western Blotting


Posted by Chris S on Dec 4, 2019 3:00:00 AM

Western blotting is used to detect proteins and analyze their expression using antibodies, but it does have its limitations. Flow cytometry also employs antibodies, but it uses fluorescence to detect and analyze protein expression on a cell-by-cell basis.

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Topics: Western Blot, Flow, techniques

Video: How to Choose Enzymatic or Sonication Protocol for ChIP


Posted by Ken B on Nov 6, 2019 3:00:00 AM

The success of your chromatin immunoprecipitation (ChIP) experiment depends on the fragmentation of chromatin, a critical step in the ChIP protocol. This can be accomplished with either sonication or enzymatic digestion. But how do you decide which chromatin fragmentation protocol to use in your ChIP experiments? A number of factors can influence your choice, making a decision seem daunting. So let’s simplify – watch the video and we'll will show you the way!

 

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Topics: Protocols, ChIP, techniques

Imaging Mass Cytometry: Endless possibilities to understand the microenvironment of disease


Posted by Kris H on Oct 23, 2019 3:15:00 AM

Understanding the microenvironment of disease is critical. Targeted cancer therapies may not be effective depending on the combination of cell types in the tumor. Likewise, an immune cell invasion in pancreatic tissue could indicate the progression of type 1 diabetes. For years, this complex landscape could only be witnessed in single-cell snapshots by flow cytometry or limited tissue staining. Imaging Mass Cytometry™ (IMC™) now offers a panorama of this dynamic world.

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Topics: IHC, techniques

General Tips for Successful Cell Culture


Posted by Dan D on Apr 24, 2019 3:10:00 AM

Culturing cells in the lab? Following these tips will add to your success and help you avoid wasted time or the dreaded cell contamination!

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Topics: techniques, Science Education, Tech Tips

STR Profiling: What's growing in your incubator?


Posted by Hayley R. on Mar 6, 2019 3:15:00 AM

Cell lines are a crucial part of life science research and development. But did you know an estimated 18-36% of cell lines are believed to be misidentified or cross-contaminated with another cell line?

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Topics: techniques, Reproducibility

Can I accelerate my ELISA results?


Posted by Chris S on Jan 16, 2019 3:10:00 AM

Running an ELISA can be a pain. Identifying pairs for an ELISA is a tedious business, and that’s before developing and validating the ELISA assay itself. Using a kit can simplify the process, but at what cost? Will that kit hinder reproducibility by introducing lot-to-lot variability over the course of my project’s lifetime? Many kits still require numerous reagent addition, incubation, and wash steps that add hands-on time and complexity to your assay.

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Topics: ELISA, techniques

When should I use flow cytometry for signaling instead of western blot?


Posted by Chris S on Jan 9, 2019 3:15:00 AM

Flow cytometry enables quantitative analysis of protein expression, signaling states, and physical characteristics (cell size/granularity) at the single-cell level. Modern flow cytometers are capable of collecting data on multiple proteins from thousands of cells per second in a heterogeneous mixture. While flow cytometry is commonly employed to identify cell types using phenotypic markers expressed on the cell surface, it can also be used to measure intracellular signaling events.

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Topics: Flow, techniques

Mycoplasma Contamination!


Posted by Michelle G on Dec 5, 2018 3:15:00 AM

Any form of cell culture contamination can ruin your day and destroy your hard work, but mycoplasma contamination is particularly devastating.

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Topics: techniques, Science Education

Writing a Grant Part 4: Additional Details


Posted by Tamar A. on Nov 7, 2018 3:10:00 AM

Finally, the finish line is approaching! You have completed your specific aims, significance and innovation, and the bulk of your research strategy. You have sent those files for numerous rounds of pre-peer review by your trusted colleagues and mentors. Now it’s time to focus on some smaller, yet still very important details.

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Topics: techniques, Career Development, Science Education

Writing a Grant Part 3: The Experimental Approach


Posted by Tamar A. on Oct 31, 2018 3:10:00 AM

In the previous post, we described how to write an effective significance and innovation section, focused on defining the problem and providing a high-level overview of your proposed solution. In this post, we’ll outline the approach, wherein you’ll expand upon the solution and illustrate exactly how you plan to conduct the research.

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Topics: techniques, Science Education

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