CST BLOG: Lab Expectations

The official blog of Cell Signaling Technology® (CST) where we discuss what to expect from your time at the bench, share tips, tricks, and information.

A Tale of Two Stains: Ponceau S vs Coomassie Blue

You've just finished running your lysate sample by SDS-PAGE, so you're pretty confident your protein...
Caroline J and Misbah K Dec 9, 2020 3:00:00 AM

Recommended Best Practices for Lysate Handling

The preparation of a cell lysate is crucial to the success of many assays, including techniques that...
Michelle G Sep 2, 2020 3:00:00 AM

4 Situations Where Flow Cytometry Might Be More Useful Than Western Blotting

Western blotting is used to detect proteins and analyze their expression using antibodies, but it do...
Chris S Dec 4, 2019 3:00:00 AM

Video: How to Choose Enzymatic or Sonication Protocol for ChIP

The success of your chromatin immunoprecipitation (ChIP) experiment depends on the fragmentation of ...
Ken B Nov 6, 2019 3:00:00 AM

Imaging Mass Cytometry: Endless possibilities to understand the microenvironment of disease

Understanding the microenvironment of disease is critical. Targeted cancer therapies may not be effe...
Kris H Oct 23, 2019 3:15:00 AM

General Tips for Successful Cell Culture

Culturing cells in the lab? Following these tips will add to your success and help you avoid wasted ...
Dan D Apr 24, 2019 3:10:00 AM

STR Profiling: What's growing in your incubator?

Cell lines are a crucial part of life science research and development. But did you know an estimate...
Hayley R. Mar 6, 2019 3:15:00 AM

Can I accelerate my ELISA results?

Running an ELISA can be a pain. Identifying pairs for an ELISA is a tedious business, and that’s bef...
Chris S Jan 16, 2019 3:10:00 AM

When should I use flow cytometry for signaling instead of western blot?

Flow cytometry enables quantitative analysis of protein expression, signaling states, and physical c...
Chris S Jan 9, 2019 3:15:00 AM

Mycoplasma Contamination!

Any form of cell culture contamination can ruin your day and destroy your hard work, but mycoplasma ...
Michelle G Dec 5, 2018 3:15:00 AM

Writing a Grant Part 4: Additional Details

Finally, the finish line is approaching! You have completed your specific aims, significance and inn...
Tamar A. Nov 7, 2018 3:10:00 AM

Writing a Grant Part 3: The Experimental Approach

In the previous post, we described how to write an effective significance and innovation section, fo...
Tamar A. Oct 31, 2018 3:10:00 AM

Writing a Grant Part 2: Significance and Innovation

The significance and innovation section is a recent (within the last 10 years) addition to the NIH a...
Tamar A. Oct 24, 2018 7:43:37 AM

Writing a Grant Part 1: First Things First

So you’re thinking of writing a grant? Or maybe your mentor has politely suggested that it would be ...
Tamar A. Oct 17, 2018 3:15:00 AM

Demystifying Multiplex IHC

In recent years, immune checkpoint proteins in the tumor microenvironment have been under intense st...
Jen Z Oct 10, 2018 3:15:00 AM

Tech Tips Video: Milk or BSA? Choosing a blocking protein for Western Blot

So you've set the timer to five minutes for the first of three TBST washes for your western blot mem...
Ken B Sep 5, 2018 3:00:00 AM

From Bench Scientist to Benchtop Support

I’m a Product Scientist at Cell Signaling Technology (CST). My typical work day no longer looks like...
Kaitlin M. Aug 29, 2018 3:15:00 AM

Painless Publication: How to Write a Conference Abstract

Our previous blog post, “Painless Publication: How to Write a Journal Abstract,” walked you through ...
Chris S Jun 27, 2018 3:15:00 AM

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