Flow cytometry enables quantitative analysis of protein expression, signaling states, and physical characteristics (cell size/granularity) at the single-cell level. Modern flow cytometers are capable of collecting data on multiple proteins from thousands of cells per second in a heterogeneous mixture. While flow cytometry is commonly employed to identify cell types using phenotypic markers expressed on the cell surface, it can also be used to measure intracellular signaling events.
Any form of cell culture contamination can ruin your day and destroy your hard work, but mycoplasma contamination is particularly devastating.
Finally, the finish line is approaching! You have completed your specific aims, significance and innovation, and the bulk of your research strategy. You have sent those files for numerous rounds of pre-peer review by your trusted colleagues and mentors. Now it’s time to focus on some smaller, yet still very important details.
In the previous post, we described how to write an effective significance and innovation section, focused on defining the problem and providing a high-level overview of your proposed solution. In this post, we’ll outline the approach, wherein you’ll expand upon the solution and illustrate exactly how you plan to conduct the research.
The significance and innovation section is a recent (within the last 10 years) addition to the NIH and most other foundation grant applications. It is a place for you to showcase WHY the work should be done – WHY there is a significant need for your study, and HOW the work is different from everyone else’s approach. What makes it groundbreaking, original research, work that will advance our scientific knowledge?
So you’re thinking of writing a grant? Or maybe your mentor has politely suggested that it would be in your best interest to do so?
Where do you start?
In recent years, immune checkpoint proteins in the tumor microenvironment have been under intense study. If you work in the immuno-oncology field, chances are you are either performing multiplex IHC (mIHC) or would like to. Ultimately, a multiplexed image like the one featured here provides a multi-layered depiction of a tumor, such that each antibody corresponds to a different fluorescent signal. If you want to detect more targets in your IHC, but aren’t sure how to design a panel of antibodies and fluorophores for mIHC, we’ll walk you through the process in this post.
So you've set the timer to five minutes for the first of three TBST washes for your western blot membrane. Now what? Sure, you could check your email or social media for the 30th time before lunch. Or you could do something informative, like check out a CST Tech Tips video! This is a new short video series featuring the same scientists who develop and validate CST antibodies, here to offer insights and protocol tips.
I’m a Product Scientist at Cell Signaling Technology (CST). My typical work day no longer looks like my fellow scientists’ daily grind, and at times, I even find it difficult to describe in words what I do, but I’ll give it a shot!
Our previous blog post, “Painless Publication: How to Write a Journal Abstract,” walked you through the steps in writing the abstract for a journal article. Now we turn our focus to writing abstracts for conference proceedings. Although there are some similarities between these two types of abstracts, there are also some distinct considerations and approaches for conference abstracts.