CST BLOG: Lab Expectations

The official blog of Cell Signaling Technology® (CST) where we discuss what to expect from your time at the bench, share tips, tricks, and information.

Using SignalScan to Investigate Infection and Immune Response

The highly contagious severe acute respiratory syndrome coronavirus-2 or SARS-CoV-2 is the cause of ...
Charles F Sep 22, 2021 3:00:00 AM

A Tale of Two Stains: Ponceau S vs Coomassie Blue

You've just finished running your lysate sample by SDS-PAGE, so you're pretty confident your protein...
Caroline J and Misbah K Dec 9, 2020 3:00:00 AM

Recommended Best Practices for Lysate Handling

The preparation of a cell lysate is crucial to the success of many assays, including techniques that...
Michelle G Sep 2, 2020 3:00:00 AM

4 Situations Where Flow Cytometry Might Be More Useful Than Western Blotting

Western blotting is used to detect proteins and analyze their expression using antibodies, but it do...
Chris S Dec 4, 2019 3:00:00 AM

Video: How to Choose Enzymatic or Sonication Protocol for ChIP

The success of your chromatin immunoprecipitation (ChIP) experiment depends on the fragmentation of ...
Ken B Nov 6, 2019 3:00:00 AM

Imaging Mass Cytometry: Endless possibilities to understand the microenvironment of disease

Understanding the microenvironment of disease is critical. Targeted cancer therapies may not be effe...
Kris H Oct 23, 2019 3:15:00 AM

General Tips for Successful Cell Culture

Culturing cells in the lab? Following these tips will add to your success and help you avoid wasted ...
Dan D Apr 24, 2019 3:10:00 AM

STR Profiling: What's growing in your incubator?

Cell lines are a crucial part of life science research and development. But did you know an estimate...
Hayley R. Mar 6, 2019 3:15:00 AM

Can I accelerate my ELISA results?

Running an ELISA can be a pain. Identifying pairs for an ELISA is a tedious business, and that’s bef...
Chris S Jan 16, 2019 3:10:00 AM

When should I use flow cytometry for signaling instead of western blot?

Flow cytometry enables quantitative analysis of protein expression, signaling states, and physical c...
Chris S Jan 9, 2019 3:15:00 AM

Mycoplasma Contamination!

Any form of cell culture contamination can ruin your day and destroy your hard work, but mycoplasma ...
Michelle G Dec 5, 2018 3:15:00 AM

Writing a Grant Part 4: Additional Details

Finally, the finish line is approaching! You have completed your specific aims, significance and inn...
Tamar A. Nov 7, 2018 3:10:00 AM

Writing a Grant Part 3: The Experimental Approach

In the previous post, we described how to write an effective significance and innovation section, fo...
Tamar A. Oct 31, 2018 3:10:00 AM

Writing a Grant Part 2: Significance and Innovation

The significance and innovation section is a recent (within the last 10 years) addition to the NIH a...
Tamar A. Oct 24, 2018 7:43:37 AM

Writing a Grant Part 1: First Things First

So you’re thinking of writing a grant? Or maybe your mentor has politely suggested that it would be ...
Tamar A. Oct 17, 2018 3:15:00 AM

Demystifying Multiplex IHC

In recent years, immune checkpoint proteins in the tumor microenvironment have been under intense st...
Jen Z Oct 10, 2018 3:15:00 AM

Tech Tips Video: Milk or BSA? Choosing a blocking protein for Western Blot

So you've set the timer to five minutes for the first of three TBST washes for your western blot mem...
Ken B Sep 5, 2018 3:00:00 AM

From Bench Scientist to Benchtop Support

I’m a Product Scientist at Cell Signaling Technology (CST). My typical work day no longer looks like...
Kaitlin M. Aug 29, 2018 3:15:00 AM

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