CST BLOG: Lab Expectations

The official blog of Cell Signaling Technology (CST), where we discuss what to expect from your time at the bench, share tips, tricks, and information.

What is glycolysis and what is its role in metabolism?

Read More
All Posts

Glycolysis and oxidative respiration are the two major metabolic pathways cells employ in order to convert biochemical energy from nutrients into adenosine triphosphate (ATP) to fuel cellular function.

  • Adenosine triphosphate (ATP) is the primary carrier of energy in all living cells.
  • ATP is made up of three components: A nitrogenous base (adenine), a sugar ribose, and a triphosphate
  • Energy is released from the breakdown of ATP to adenosine diphosphate (ADP) via hydrolysis to fuel various cellular processes.

What role does glycolysis play in metabolism?

Glycolysis is the metabolic pathway that converts glucose into pyruvate.

  • Occurs in the cytosol and is oxygen-independent
  • The free energy released during the biochemical reactions in glycolysis is used to generate a net gain of two molecules of ATP.
  • Pyruvate generated via glycolysis is transported into mitochondria where it enters the tricarboxylic acid (TCA) cycle under normoxic conditions, or is converted to lactate when oxygen levels are low.
  • Under hypoxic conditions (low O2), rates of glycolysis increase to compensate for decreased oxidative respiration to fulfill cellular energy demands.

20-ODA-53110 Mitochondria_resized

Oxidative Metabolism vs Glycolysis

Oxidative respiration is the primary mechanism that cells use to release chemical energy stored in nutrients (primarily glucose) to fuel cellular activity.

  • Occurs in mitochondria, and, as its name implies, requires oxygen.  
  • Acetyl-CoA is produced from pyruvate molecules generated via glycolysis and enters the TCA cycle to generate the high-energy molecules NADH, FADH2, and ATP.
  • More efficient than glycolysis: oxidative respiration yields 30-36 molecules of ATP per glucose molecule.

Although oxidative respiration is the predominant metabolic energy-producing pathway in normal cells, cancer cells reprogram their metabolism and more readily rely on glycolysis, even in the presence of oxygen, for energy production. This phenomenon is known as the Warburg effect.

Antibody Sampler Kits for Studying Glycolysis & Metabolism 

Chris Sumner
Chris Sumner
Chris Sumner was a writer at CST. When he's not reading/writing about curing disease, he's hiking in the woods, playing guitar, or searching for the world's best lobster roll.

Recent Posts

Mechanisms of Cell Death: Apoptosis

What is apoptosis? Apoptosis is a form of programmed cell death (PCD) characterized by cell shrinkage, ch...
Gary Kasof, PhD Jun 18, 2025

Mapping RNA-Protein Interactions with RBP-eCLIP: Powering Discovery in RNA Biology

Guest author Dr Vince Harjono is an Application Scientist Manager at Eclipsebio. CST and Eclipsebio have ...

Key Epithelial-Mesenchymal Transition (EMT) Markers in Cancer

EMT, or epithelial-mesenchymal transition, is a biological process in which epithelial cells gain the abi...
Susan Keezer, PhD Jun 4, 2025