Nature Communications recently published a novel study from the laboratory of Daniel Figeys at the University of Ottowa, which begins to unravel the complexity of the microbiome of pediatric patients with Crohn’s disease or CD, which is a type of inflammatory bowel disease (IBD). It causes inflammation of your digestive tract, which can cause symptoms like abdominal pain, severe diarrhea, fatigue, weight loss, and malnutrition.
Case Study: Acetylated Lysine and Its Impact on Crohn’s Disease
Posted by Charles F and Josh N on Dec 16, 2020 3:00:00 AM
Taking Out the Mitochondria in Parkinson’s Disease
Posted by Richard C and Yiying Z on Jul 31, 2019 3:15:00 AM
Parkinson’s disease (PD) is a neurodegenerative disease marked by loss of dopaminergic neurons in the substantia nigra. Mutations in the gene that encodes a ubiquitin ligase PARK2/Parkin are known to cause autosomal recessive forms of familial PD1. Parkin plays a key role in mitochondrial homeostasis by regulating a specialized form of autophagy called mitophagy, the clearance of defective or damaged mitochondria by lysosomes2.
How does altered mitochondrial homeostasis contribute to PD?
Topics: Proteomics, Neurodegeneration
Webinar | Proteomics profiling of post-translational modifications in early drug discovery
Posted by Chris S on Mar 28, 2018 3:15:00 AM
Advances in mass spectrometry instrumentation and sample handling methods have propelled proteomics and extended its utility for both basic biology and early drug development. Changes in protein abundance and post-translational modification state often reflect the activity of a novel therapeutic agent as well as the sensitivity/resistance of a biological system to treatment.
Topics: Proteomics, Webinars
The Potential of Proteomics: A Clear Path to Discovery | Part II
Posted by Carolyn P on Feb 22, 2017 3:00:00 AM
See a real life example of how PTMScan technology can facilitate translational discovery. In this short video we describe how CST not only identified a major driver of NSCLC, which can respond to an FDA approved drug, but went a step further and developed an antibody that can be used to test which patients might be candidates for treatment. All thanks to the power of simplified proteomics.
Topics: Proteomics, PTMScan, techniques
Part 1 gave an overview on mass spectrometry-based proteomics. Now it’s time to talk about how this strategy can be used to identify peptides with post-translational modifications (PTM) from a complex biological sample.
Topics: Primary Antibodies, Post Translational Modification, Proteomics
The Potential of Proteomics: A Clear Path to Discovery
Posted by Carolyn P on Jan 11, 2017 3:00:00 AM
Whether your lab is well-versed and equipped to do Mass Spec analysis in-house or your lab has never considered setting up a proteomics experiment before, the Proteomics group at CST has a solution to help clear the way. Click below to watch the video and learn more.
Topics: Proteomics, PTMScan, techniques
After sequencing of the human genome was complete, it was time to roll up our sleeves and get started on the daunting task of unraveling the complexity of the proteome. Thus the era of proteomics, the study of the function of all expressed proteins, was born. This task is especially complicated
Post Translational Modification: Antibody Enrichment for Mass Spectrometry-based Proteomics
Continuing our theme of simplifying proteomics we present a webinar featuring Matthew Stokes, Ph.D., principal scientist of the proteomics group here at Cell Signaling Technology and Christopher Rose, Ph.D., a postdoctoral research fellow in the Gygi Lab at Harvard Medical School.
Dr. Stokes describes PTMScan® technology, a method that uses antibodies to enrich specific post-translationally modified (PTM) peptides (e.g., phosphorylated, methylated, ubiquitinated etc) from a complex mixture prior to LC-MS/MS analysis. Dr. Rose demonstrates how, by combining PTMScan technology with isobaric labeling, specifically with tandem mass tags (TMTs), his lab quantified over 15,000 ubiquitination events in Bortezomib treated cells.
Topics: Protocols, Cancer Research, Proteomics, PTMScan
Topics: Primary Antibodies, Post Translational Modification, Proteomics
Part 1 gave an overview on mass spectrometry-based proteomics. Now it’s time to talk about how this strategy can be used to identify peptides with post-translational modifications (PTM) from a complex biological sample.
Topics: Primary Antibodies, Post Translational Modification, Proteomics