CST BLOG: Lab Expectations

The official blog of Cell Signaling Technology® (CST) where we discuss what to expect from your time at the bench, share tips, tricks, and information.

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When Specificity Is Simply Sweet

Rajan Burt, Samuel Myers, and colleagues from the La Jolla Institute for Immunology, Broad Institute, Cell Signaling Technology, and Yale University combined their expertise to successfully pioneer a new path towards identifying the ubiquitous protein modification O-GlcNAcylation. 

 

The story is an old one, as the simple sugar or monosaccharide O-GlcNAc was identified almost 40 years ago by Torres and Hart in 1984. O-GlcNAc is the one of the most common post-translational modifications (or PTM), it’s found everywhere on proteins inside the cell, including the nucleus, cytoplasm and mitochondria. Not to be confused with the highly complexed N-linked sugars present on proteins that face the extracellular milieu, O-GlcNAc is a simple monosaccharide involved in the most complex biological pathways involving the epigenetic regulation of transcription, stress response, translation, protein degradation and homeostasis.

 

Because it functions as a cellular sensor for nutrient levels, O-GlcNAc serves as an integrator of multiple signaling pathways. Remarkably, O-GlcNAcylation cell-wide is controlled by only two proteins, OGT that adds the monosaccharide to serine and threonine residues and OGA that enzymatically removes the PTM. Newer, more holistic models of signal transduction shy away from merely linear models of activation and inhibition to models that incorporate multiple pathways as well as multiple modes of feed-back and cross talk. This explains in part how one PTM can be involved in seemingly diverse diseases like diabetes, neurodegeneration, and cancer in humans.

 

The key to the understanding the role of O-GlcNAc in biology and disease is reliably identifying the PTM. And scientists at Cell Signaling Technology have created a mixture of sensitive, specific rabbit monoclonal antibodies that can immunoprecipitate in one step the O-GlcNAcylated peptides derived from the parent proteins. This new antibody-based tool coupled with advances in mass spectrometry developed by scientists at the Broad Institute allow for the identification of over a thousand unique O-GlcNAcylated sites in one enrichment step.

 

Furthermore, the sensitivity and specificity of the O-GlcNAc antibody was demonstrated by a strong preference for O-GlcNAc as compared to its epimer O-GalNAc. The technique may be readily applied to native O-GlcNAc modified peptides, allowing investigators to explore O-GlcNAc questions in vivo and potentially from patient derived samples. So, without complicated and time-consuming chemical derivatization, using 10-fold less sample than previous experiments with significantly less mass spectrometer time this study demonstrates the synergy of highly specific reagents and innovative mass spectrometry in creating a pathway for discovery.

 

Read more about this research.

Charles F
Charles F
Chuck Farnsworth is a proteomics application scientist; his focus is to assist scientists around the world get the best data possible using CST’s proteomics kits. Camping with the kids and stargazing in the Mad River Valley of Vermont are his favorite things to do.

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