CST BLOG: Lab Expectations

The official blog of Cell Signaling Technology (CST), where we discuss what to expect from your time at the bench, share tips, tricks, and information.

Lab Expectations listing page

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Journal Club: TREM2 Opens a Rabbit Hole of Questions for Alzheimer’s Researchers

It's almost time for the 2017 Society for Neuroscience meeting. To get your neurons excited for the ...
Richard Cho, PhD Nov 8, 2017

Western Blot Normalization: Am I setting up my loading controls correctly?

You’re gathering data from all your experiments and preparing to present to your advisor and thesis ...
Kenneth Buck, PhD Nov 1, 2017

Journal Club: Hippo pathway and intestinal stem cell regeneration

Journal Club: YAP-dependent reprogramming of Lgr5+ stem cells drives intestinal regeneration and can...
Liana G Sep 20, 2017

Fluorescent Staining Using Multiple Antibodies: Two Common Techniques

The use of multiple antibodies in a single experiment can provide useful information to researchers....
Tara White Sep 13, 2017

Switching from Manual to Automated IHC? We've got your back

If you’ve ever transitioned your IHC experiments from a manual protocol to an automated platform, yo...
Chris G Aug 30, 2017

CST Interns: A Fun and Productive Summer for Future STEM Leaders

The development and testing of new products at Cell Signaling Technology (CST) involves the work of ...
Kenneth Buck, PhD Aug 23, 2017

Have You Ever Wondered: What's The Lowdown on Phospho-Specific Antibodies?

Early exploration of unmapped biological signaling pathways was carried out using radiolabeled phosp...
Kenneth Buck, PhD Jul 26, 2017

Successful Immunofluorescence: Antibody Dilution & Incubation Conditions

Part four of a four-part series on successful immunofluorescence. Read Part 1: The Importance of Val...
Kenneth Buck, PhD May 10, 2017

In-depth Characterization of Immune Checkpoints in the Tumor Microenvironment

Characterize Immune Checkpoint Proteins & T Cell Exhaustion Using Multiplex IHC Advances in immu...
Chris Sumner May 3, 2017

Journal Club | The genomic address of bivalent nucleosomes

The activity of genes and their regulatory elements is, in part, governed by their cell type-specifi...
Liana G Apr 26, 2017

Journal Club | Zika virus turns off Akt signaling to hijack autophagy in developing neural tissue

The Zika virus (ZIKV) is related to flaviviruses that cause dengue and yellow fever, and is spread v...
Kenneth Buck, PhD Mar 29, 2017

Successful Immunofluorescence: Fixation and Permeabilization

Part Three of a four-part series on successful immunofluorescence. Read Part 1: The Importance of Va...
Kenneth Buck, PhD Mar 15, 2017

Successful Immunofluorescence: Experimental Controls

Part two of a four-part series on successful immunofluorescence. Read Part 1: The Importance of Vali...
Kenneth Buck, PhD Mar 8, 2017

Successful Immunofluorescence: The Importance of Validation

Part 1 of a four-part series on successful immunofluorescence. Read Part 2: Experimental Controls, P...
Kenneth Buck, PhD Mar 1, 2017

How to Validate a Histone Modification Antibody

Antibodies targeted to histone modifications may bind non-specifically to similar, but off-target hi...
Carolyn P Feb 1, 2017

Beat the Blues of Writing a Scientific Rebuttal

You are glaring at your computer screen with a million thoughts in your head. You just got back the ...
Neha G Jan 18, 2017

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