CST BLOG: Lab Expectations

The official blog of Cell Signaling Technology® (CST) where we discuss what to expect from your time at the bench, share tips, tricks, and information.

All Posts

In-depth characterization of immune checkpoints in the tumor microenvironment

Characterize Immune Checkpoint Proteins and T Cell Exhaustion
Using Multiplex IHC


Advances in immuno-oncology have successfully led to novel cancer therapeutics with favorable patient responses that are more durable than conventional cytotoxic chemotherapy (1). However, not all patients respond to immunotherapy; therefore investigators are trying to identify clinically relevant biomarkers with the goal of developing therapeutics based on personalized medicine (2,3).
Download our mIHC app notes and posters

Download our mIHC app notes and posters.

Spatial localization of multiple biomarkers is critical when cataloging subsets of immune infiltrate and cancer cells and their interactions in the tumor microenvironment. Multiplexed assays are required for investigations of multiple therapeutic targets and predictive biomarkers in limited and valuable patient samples. For these reasons, fluorescent multiplex immunohistochemistry (mIHC), which enables detection of 6 or more proteins/biomarkers in formalin-fixed, paraffin-embedded (FFPE) tissue samples, is a valuable tool for immuno-oncology.

In mIHC as well as in single/dual-plex chromogenic IHC approaches, using application-validated antibodies against relevant targets is crucial in order to obtain reliable results. Antibodies validated for IHC from CST enable investigators to get more information about biomarker expression, localization, interaction, and disease context.

Our mIHC app note and poster resources explore the protocol and technical considerations for selecting and using antibodies in mIHC to assess immune checkpoint proteins and T cell exhaustion in FFPE tissue samples.

References:
1. Sharma, P. and Allison, J.P. (2015) Cell 161, 205–14.
2. Mahoney, K.M. and Atkins, M.B. (2014) Oncology (Williston Park) 28 (suppl 3), 39–48.
3. Masucci, G.V. et al. (2016) J Immunother Cancer 4, 76.

Chris S
Chris S
Chris Sumner is the Editor-in-Chief of Lab Expectations. When he's not reading/writing about curing disease, he's hiking in the woods, playing guitar, or searching for the world's best lobster roll.

Related Posts

Why use viability dyes in flow cytometry experiments?

Viability dyes are widely used when counting and passaging cells, assessing chemical toxicity, and perfor...
Rob M Nov 17, 2021 3:00:00 AM

Rising Black Scientist Round Table

The Rising Black Scientists Awards were created by Cell Signaling Technology and Cell Press in 2020 to br...
Chris S Nov 8, 2021 8:15:00 AM

Emerging Discoveries in ER-Phagy

Autophagy is a catabolic process for the disposal of cytoplasmic contents that are captured by double-mem...
Gary K. Nov 3, 2021 3:00:00 AM