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The official blog of Cell Signaling Technology, where we discuss what to expect from your time at the bench, share tips, tricks, and information.

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Successful Immunofluorescence: Fixation and Permeabilization

Part Three of a four-part series on successful immunofluorescence. Read Part 1: The Importance of Va...
Kenneth Buck, PhDMar 15, 2017

Successful Immunofluorescence: Experimental Controls

Part two of a four-part series on successful immunofluorescence. Read Part 1: The Importance of Vali...
Kenneth Buck, PhDMar 8, 2017

Successful Immunofluorescence: The Importance of Validation

Part 1 of a four-part series on successful immunofluorescence. Read Part 2: Experimental Controls, P...
Kenneth Buck, PhDMar 1, 2017

Beat the Blues of Writing a Scientific Rebuttal

You are glaring at your computer screen with a million thoughts in your head. You just got back the ...

The Questions to Ask Before Starting Your Next IF Experiment

Specificity, consistency, and optimized assay conditions are three key elements that help ensure rel...

Understanding the Proteome: An Introduction to Mass Spectrometry-Based Proteomics

After sequencing of the human genome was complete, it was time to roll up our sleeves and get starte...
Claire SJun 15, 2016

From Discovery to Diagnostics: Translational Applications of PTM Proteomics

Part 1 and part 2 introduced mass spectrometry-based proteomic methods, like PTMScan® technology, fo...
Claire SDec 9, 2015

Enriching for PTMs: Antibody-Based Strategies for Proteomics Analysis

In part one, Understanding the Proteome: An Introduction to Mass Spectrometry-Based Proteomics, we g...
Claire SDec 2, 2015

Five Steps to Publication-Worthy IF Images

A picture is worth a thousand words, or in the case of immunofluorescent (IF) imaging, a thousand pr...
Tara WhiteSep 23, 2015

Monoclonal Antibody History: How was antibody technology discovered?

Today, monoclonal antibodies are a ubiquitous research tool. In fact, you’d be hard-pressed to find ...
Carolyn PAug 26, 2015

The Cost of a Failed Western Blot

Thinking about lab expenses isn’t as enjoyable as investigating your favorite signaling pathway. How...
Claire SAug 13, 2015

Four Steps to Better Immunohistochemistry: Steps 2, 3, and 4

This is part two of a two-part series on how to optimize your immunohistochemistry (IHC) protocols. ...
Carolyn PJul 15, 2015

Four Steps to Better Immunohistochemistry: Step 1 - Antigen Retrieval

It’s Friday night and you could be out with your friends right now, but instead you’re tucked away i...
Carolyn PJul 7, 2015

From PCR to ChIP-seq: How to Analyze Your ChIP Data

Part one of this series described the importance of including proper controls in your protocols. In ...
Carolyn PMar 11, 2015

Signal vs Noise: Choosing the Right Antibody for Successful ChIP

Part one of this series described the importance of including proper controls to your protocols. In ...
Carolyn PMar 4, 2015

Take Control: How to Use Positive and Negative Controls for Better ChIP Results

You're a confocal maestro, playing the lasers and the filters and the gain settings like a Stradivar...
Carolyn PFeb 18, 2015

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