CST BLOG: Lab Expectations

The official blog of Cell Signaling Technology (CST), where we discuss what to expect from your time at the bench, share tips, tricks, and information.

Increase Your Experimental Success with CUT&RUN-Validated Antibodies

CUT&RUN delivers the power of chromatin immunoprecipitation (ChIP) or ChIP-sequencing (ChIP-seq)...
Christopher Fry, PhD Oct 26, 2022

Tools to Perform Genomic Profiling

Techniques used in genomic profiling have come a long way in the past few decades. Researchers can n...
Curtis Desilets Dec 1, 2021

Video: How to Choose Enzymatic or Sonication Protocol for ChIP

The success of your chromatin immunoprecipitation (ChIP) experiment depends on the fragmentation of ...
Kenneth Buck, PhD Nov 6, 2019

Preparing a ChIP-seq DNA library. Where do I start?

Chromatin immunoprecipitation sequencing (ChIP-seq) is a flexible and powerful technique used by res...
Tamar A Sep 18, 2019

How to Choose Enzymatic or Sonication Protocol for ChIP

Researchers use chromatin immunoprecipitation, or ChIP, to identify and characterize protein-DNA int...
Chris Sumner Jun 26, 2019

Tech Tips Video: How much antibody should I use for ChIP?

It's time to check out another video from the CST Tech Tips playlist! In this edition of Tech Tips, ...
Kenneth Buck, PhD Nov 28, 2018

Myth-busting for ChIP/ChIP-seq: Using Mono vs Polyclonal Antibodies

Researchers who run a lot of chromatin immunoprecipitation "ChIP" assays—maybe even your advisor—mig...
Kenneth Buck, PhD Sep 26, 2018

Keep ChIP’ing Away

So your experiments and data are funneling you down an inescapable path. You need to show direct gen...
Curtis Desilets Jan 31, 2018

Discovery Through Validation: ChIP Assays

Cell Signaling Technology is proud to present the following On-Demand Webinar: The Use of Highly Val...
Carolyn P Aug 2, 2017

Need Better ChIP Results? Use Enzymatic Digestion

The success or failure of a chromatin immunoprecipitation (ChIP) experiment is highly dependent on t...
Carolyn P Aug 5, 2015

4 Steps to Better ChIP results - Step 4 - Downstream Analysis

Part one of this series described the importance of including proper controls to your protocols. In ...
Carolyn P Mar 11, 2015

4 Steps to Better ChIP results - Step 3- Choosing an Antibody

Part one of this series described the importance of including proper controls to your protocols. In ...
Carolyn P Mar 4, 2015

4 Steps to Better ChIP results - Step 2- Prep well

Part one of this series described the importance of including proper controls to your protocols. Now...
Carolyn P Feb 25, 2015

4 Steps to Better ChIP results - Step 1- Take control

Introduction You're a confocal maestro, playing the lasers and the filters and the gain settings lik...
Carolyn P Feb 18, 2015

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