CST BLOG: Lab Expectations

The official blog of Cell Signaling Technology (CST), where we discuss what to expect from your time at the bench, share tips, tricks, and information.

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ChIP, CUT&RUN & CUT&Tag: Support Reagents for Successful Chromatin Profiling

If you’ve performed CUT&RUN, CUT&Tag, or ChIP/ChIP-seq experiments, you know how important i...
Andrea Tu, PhD May 8, 2024

Tools to Perform Genomic Profiling

Techniques used in genomic profiling have come a long way in the past few decades. Researchers can n...
Curtis Desilets Dec 1, 2021

How to Prepare a ChIP-seq DNA Library

Chromatin immunoprecipitation sequencing (ChIP-seq) is a flexible and powerful technique used by res...
Tamar Aprahamian, PhD Sep 18, 2019

How to Choose Enzymatic or Sonication Protocol for ChIP

Researchers use chromatin immunoprecipitation, or ChIP, to identify and characterize protein-DNA int...
Chris Sumner Jun 26, 2019

Tech Tips Video: How much antibody should I use for ChIP?

It's time to check out another video from the CST Tech Tips playlist! In this edition of Tech Tips, ...
Kenneth Buck, PhD Nov 28, 2018

Myth-busting for ChIP/ChIP-seq: Using Mono vs Polyclonal Antibodies

Researchers who run a lot of chromatin immunoprecipitation "ChIP" assays—maybe even your advisor—mig...
Kenneth Buck, PhD Sep 26, 2018

From PCR to ChIP-seq: How to Analyze Your ChIP Data

Part one of this series described the importance of including proper controls to your protocols. In ...
Carolyn P Mar 11, 2015

Signal vs Noise: Choosing the Right Antibody for Successful ChIP

Part one of this series described the importance of including proper controls to your protocols. In ...
Carolyn P Mar 4, 2015

Enzymatic vs Sonication: Optimizing Chromatin Prep for Successful ChIP

Part one of this series described the importance of including proper controls in your protocols. Now...
Carolyn P Feb 25, 2015

Take Control: How to Use Positive and Negative Controls for Better ChIP Results

You're a confocal maestro, playing the lasers and the filters and the gain settings like a Stradivar...
Carolyn P Feb 18, 2015

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