CST BLOG: Lab Expectations

The official blog of Cell Signaling Technology (CST), where we discuss what to expect from your time at the bench, share tips, tricks, and information.

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Beyond Your Primary Antibody: What You Need for a Successful Chromatin Profiling Experiment

If you’ve performed CUT&RUN, CUT&Tag, or ChIP/ChIP-seq experiments, you know how important i...
Andrea Tu, PhD May 8, 2024

How to Control Your Apoptosis, Autophagy, and Mitophagy Western Blots

Have you ever spent time treating cells, preparing cell extracts, and performing a western blot only...
Whitney Palmer Dec 4, 2023

Why use viability dyes in flow cytometry experiments?

Viability dyes are widely used when counting and passaging cells, assessing chemical toxicity, and p...
Rob MacDonald, PhD Nov 17, 2021

Tools for Modulating Cells

Cell behavior is a tightly regulated, finely tuned process where various different signals work toge...
Andrea Tu, PhD Oct 13, 2021

A Tale of Two Stains: Ponceau S vs Coomassie Blue

You've just finished running your lysate sample by SDS-PAGE, so you're pretty confident your protein...

Recommended Best Practices for Lysate Handling

The preparation of a cell lysate is crucial to the success of many assays, including techniques that...
Michelle G Sep 2, 2020

Loading Control Expression in Mouse Tissues

When it comes to mouse tissue samples, not all western blot loading control proteins are expressed e...
Supriya Singh, PhD Jun 24, 2020

Milk or BSA? How to Choose a Blocking Protein for Western Blot [Video]

So you've set the timer to five minutes for the first of three TBST washes for your western blot mem...
Kenneth Buck, PhD Sep 5, 2018

Western Blot Normalization: Am I setting up my loading controls correctly?

You’re gathering data from all your experiments and preparing to present to your advisor and thesis ...
Kenneth Buck, PhD Nov 1, 2017

Four Steps to Better Immunohistochemistry: Step 1 - Antigen Retrieval

It’s Friday night and you could be out with your friends right now, but instead you’re tucked away i...
Carolyn P May 24, 2017

Successful Immunofluorescence: Antibody Dilution & Incubation Conditions

Part four of a four-part series on successful immunofluorescence. Read Part 1: The Importance of Val...
Kenneth Buck, PhD May 10, 2017

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