CST BLOG: Lab Expectations

The official blog of Cell Signaling Technology (CST), where we discuss what to expect from your time at the bench, share tips, tricks, and information.

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Western Blot Normalization: Am I setting up my loading controls correctly?

You’re gathering data from all your experiments and preparing to present to your advisor and thesis ...
Kenneth Buck, PhD Nov 1, 2017

Journal Club: Hippo pathway and intestinal stem cell regeneration

Journal Club: YAP-dependent reprogramming of Lgr5+ stem cells drives intestinal regeneration and can...

Fluorescent Staining Using Multiple Antibodies: Two Common Techniques

The use of multiple antibodies in a single experiment can provide useful information to researchers....
Tara White Sep 13, 2017

What Are Phospho-Specific Antibodies?

Early exploration of unmapped biological signaling pathways was carried out using radiolabeled phosp...
Kenneth Buck, PhD Jul 26, 2017

Successful Immunofluorescence: Antibody Dilution & Incubation Conditions

Part four of a four-part series on successful immunofluorescence. Read Part 1: The Importance of Val...
Kenneth Buck, PhD May 10, 2017

Journal Club | The genomic address of bivalent nucleosomes

The activity of genes and their regulatory elements is, in part, governed by their cell type-specifi...

Successful Immunofluorescence: Fixation and Permeabilization

Part Three of a four-part series on successful immunofluorescence. Read Part 1: The Importance of Va...
Kenneth Buck, PhD Mar 15, 2017

Successful Immunofluorescence: Experimental Controls

Part two of a four-part series on successful immunofluorescence. Read Part 1: The Importance of Vali...
Kenneth Buck, PhD Mar 8, 2017

Successful Immunofluorescence: The Importance of Validation

Part 1 of a four-part series on successful immunofluorescence. Read Part 2: Experimental Controls, P...
Kenneth Buck, PhD Mar 1, 2017

Beat the Blues of Writing a Scientific Rebuttal

You are glaring at your computer screen with a million thoughts in your head. You just got back the ...

The Questions to Ask Before Starting Your Next IF Experiment

Specificity, consistency, and optimized assay conditions are three key elements that help ensure rel...

The Reproducibility Crisis: An Open Letter from Our Chief Scientific Officer

A Message from our Chief Scientific Officer, Roberto Polakiewicz, Ph.D. The time is now for the scie...
Carolyn P Aug 10, 2016

Understanding the Proteome: An Introduction to Mass Spectrometry-Based Proteomics

After sequencing of the human genome was complete, it was time to roll up our sleeves and get starte...
Claire S Jun 15, 2016

CRISPR: Science Magazine’s 2015 Breakthrough of the Year

CRISPR, a nifty gene-editing tool, has swept the scientific community off its feet. It has a catchy ...

From Discovery to Diagnostics: Translational Applications of PTM Proteomics

Part 1 and part 2 introduced mass spectrometry-based proteomic methods, like PTMScan® technology, fo...
Claire S Dec 9, 2015

Enriching for PTMs: Antibody-Based Strategies for Proteomics Analysis

In part one, Understanding the Proteome: An Introduction to Mass Spectrometry-Based Proteomics, we g...
Claire S Dec 2, 2015

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