CST BLOG: Lab Expectations

The official blog of Cell Signaling Technology (CST), where we discuss what to expect from your time at the bench, share tips, tricks, and information.

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The Questions to Ask Before Starting Your Next IF Experiment

Specificity, consistency, and optimized assay conditions are three key elements that help ensure rel...

Understanding the Proteome: An Introduction to Mass Spectrometry-Based Proteomics

After sequencing of the human genome was complete, it was time to roll up our sleeves and get starte...
Claire S Jun 15, 2016

From Discovery to Diagnostics: Translational Applications of PTM Proteomics

Part 1 and part 2 introduced mass spectrometry-based proteomic methods, like PTMScan® technology, fo...
Claire S Dec 9, 2015

Enriching for PTMs: Antibody-Based Strategies for Proteomics Analysis

In part one, Understanding the Proteome: An Introduction to Mass Spectrometry-Based Proteomics, we g...
Claire S Dec 2, 2015

Five Steps to Publication-Worthy IF Images

A picture is worth a thousand words, or in the case of immunofluorescent (IF) imaging, a thousand pr...
Tara White Sep 23, 2015

History of the Monoclonal Antibody - Part I

Monoclonal antibodies are a pretty ubiquitous research tool. In fact, you’d be hard-pressed to find ...
Carolyn P Aug 26, 2015

The Cost of a Failed Western Blot

Thinking about lab expenses isn’t as enjoyable as investigating your favorite signaling pathway. How...
Claire S Aug 13, 2015

Four Steps to Better Immunohistochemistry: Steps 2, 3, and 4

This is part two of a two-part series on how to optimize your immunohistochemistry (IHC) protocols. ...
Carolyn P Jul 15, 2015

Four Steps to Better Immunohistochemistry: Antigen Retrieval

It’s Friday night and you could be out with your friends right now, but instead you’re tucked away i...
Carolyn P Jul 7, 2015

From PCR to ChIP-seq: How to Analyze Your ChIP Data

Part one of this series described the importance of including proper controls in your protocols. In ...
Carolyn P Mar 11, 2015

Signal vs Noise: Choosing the Right Antibody for Successful ChIP

Part one of this series described the importance of including proper controls to your protocols. In ...
Carolyn P Mar 4, 2015

Take Control: How to Use Positive and Negative Controls for Better ChIP Results

You're a confocal maestro, playing the lasers and the filters and the gain settings like a Stradivar...
Carolyn P Feb 18, 2015

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