CST BLOG

The official blog of Cell Signaling Technology, where we discuss what to expect from your time at the bench, share tips, tricks, and information.

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Dual Staining Mouse CD8 and FoxP3 with Confidence

For decades, immunohistochemistry (IHC) has been a powerful technique for the investigation and visu...
Tamar Aprahamian, PhD Sep 25, 2019

How to Prepare a ChIP-seq DNA Library

Chromatin immunoprecipitation sequencing (ChIP-seq) is a flexible and powerful technique used by res...
Tamar Aprahamian, PhD Sep 18, 2019

Hallmarks of Cancer: Tumor-Promoting Inflammation

Cancer cells hijack inflammatory mechanisms to promote their own growth and survival. During a norma...
Chris Sumner Aug 21, 2019

Taking Out the Mitochondria in Parkinson’s Disease

We’re proud to partner with The Michael J. Fox Foundation for Parkinson’s Research (MJFF) to move Pa...
Richard Cho, PhD Jul 31, 2019

The BAFfling Complexity of the BAF Complex in Cancer

How do cells dynamically regulate access to their tightly packed DNA to control gene expression and ...
Curtis Desilets Jul 24, 2019

Hallmarks of Cancer: Activating Invasion and Metastasis

Cancer cells invade local tissue and spread to distant sites via two distinct, but similar processes...
Chris Sumner Jul 17, 2019

Enzymatic vs Sonication: Optimizing Chromatin Prep for Successful ChIP

Part one of this series described the importance of including proper controls in your ChIP protocols...
Carolyn P Jun 26, 2019

Tissue Clearing Imaging Methods for Whole, Intact Brain Imaging

For generations of neuroscientists, using immunohistochemistry to study the brain in its anatomical ...
Richard Cho, PhD Jun 5, 2019

Pathways to Cancer: Epithelial-Mesenchymal Transition

The process of epithelial-mesenchymal transition (EMT), whereby differentiated epithelial cells tran...
Antony Wood, PhD May 29, 2019

Need cell type and function? Use flow cytometry

What is flow cytometry and how is it used? Flow cytometry enables you to save time and analyze many ...
Tamar Aprahamian, PhD May 8, 2019

Hallmarks of Cancer: Evading Growth Suppressors

Cancer cells resist inhibitory signals that might otherwise stop their growth. The major pathways in...
Chris Sumner May 1, 2019

General Tips for Successful Cell Culture

Culturing cells in the lab? Following these tips will add to your success and help you avoid wasted ...

Hallmarks of Cancer: Enabling Replicative Immortality

Cancer cells can revert to a pre-differentiated, stem-cell-like phenotype, allowing uninhibited cell...
Chris Sumner Apr 17, 2019

Hallmarks of Cancer: Sustaining Proliferative Signaling

Cancer cells stimulate their own growth, which means they become self-sufficient in growth signals a...
Chris Sumner Apr 3, 2019

Hypoxia & Cancer: The role of HIF-1α in Oxygen Sensing, Metabolism, and Tumorigenesis

Molecular oxygen (O2) is an essential element for metazoan life. Among its many roles, O2 functions ...
Jianxin Xie, PhD Mar 27, 2019

Necroptosis & Pyroptosis Add to Our Understanding of Apoptotic Cell Death

Over the last 50 years, laboratories have been able to demonstrate through experimentation the proce...
Gary Kasof, PhD Mar 13, 2019

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