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The official blog of Cell Signaling Technology, where we discuss what to expect from your time at the bench, share tips, tricks, and information.

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Dual Staining Mouse CD8 and FoxP3 with Confidence

For decades, immunohistochemistry (IHC) has been a powerful technique for the investigation and visu...
Tamar Aprahamian, PhD Sep 25, 2019

How to Prepare a ChIP-seq DNA Library

Chromatin immunoprecipitation sequencing (ChIP-seq) is a flexible and powerful technique used by res...
Tamar Aprahamian, PhD Sep 18, 2019

Taking Out the Mitochondria in Parkinson’s Disease

We’re proud to partner with The Michael J. Fox Foundation for Parkinson’s Research (MJFF) to move Pa...
Richard Cho, PhD Jul 31, 2019

The BAFfling Complexity of the BAF Complex in Cancer

How do cells dynamically regulate access to their tightly packed DNA to control gene expression and ...
Curtis Desilets Jul 24, 2019

Enzymatic vs Sonication: Optimizing Chromatin Prep for Successful ChIP

Part one of this series described the importance of including proper controls in your ChIP protocols...
Carolyn P Jun 26, 2019

Tissue Clearing Imaging Methods for Whole, Intact Brain Imaging

For generations of neuroscientists, using immunohistochemistry to study the brain in its anatomical ...
Richard Cho, PhD Jun 5, 2019

Pathways to Cancer: Epithelial-Mesenchymal Transition

The process of epithelial-mesenchymal transition (EMT), whereby differentiated epithelial cells tran...
Antony Wood, PhD May 29, 2019

Need cell type and function? Use flow cytometry

What is flow cytometry and how is it used? Flow cytometry enables you to save time and analyze many ...
Tamar Aprahamian, PhD May 8, 2019

General Tips for Successful Cell Culture

Culturing cells in the lab? Following these tips will add to your success and help you avoid wasted ...

Hypoxia & Cancer: The role of HIF-1α in Oxygen Sensing, Metabolism, and Tumorigenesis

Molecular oxygen (O2) is an essential element for metazoan life. Among its many roles, O2 functions ...
Jianxin Xie, PhD Mar 27, 2019

Necroptosis & Pyroptosis Add to Our Understanding of Apoptotic Cell Death

Over the last 50 years, laboratories have been able to demonstrate through experimentation the proce...
Gary Kasof, PhD Mar 13, 2019

STR Profiling: What's Growing in Your Incubator?

Cell lines are a crucial part of life science research and development. But did you know an estimate...

Studying Post-translationally Modified Sites and Disease Variants with PhosphoSitePlus®

Post-translational modifications (PTMs) are employed in the cell to alter protein function, and typi...

More Than a Phenotype: Assessing Tumor-infiltrating Immune Cell Type & Function

Although the rise of cancer immunotherapy may seem meteoric to many, the history actually stretches ...
Emily Alonzo, PhD Feb 6, 2019

What makes an antibody monoclonal, polyclonal, or recombinant?

When you’re shopping for antibodies, there are so many factors to consider. For example, will it wor...
Chris Sumner Jan 30, 2019

Can I accelerate my ELISA results?

Running an ELISA can be a pain. Identifying pairs for an ELISA is a tedious business, and that’s bef...
Chris Sumner Jan 16, 2019

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