CST BLOG: Lab Expectations

The official blog of Cell Signaling Technology (CST), where we discuss what to expect from your time at the bench, share tips, tricks, and information.

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More Than a Phenotype: A Guide to Assessing Tumor-infiltrating Immune Cell Types and Functions

Although the rise of cancer immunotherapy may seem meteoric to many, the history goes back over 100 ...
Emily Alonzo, PhD Feb 6, 2019

What makes an antibody monoclonal, polyclonal, or recombinant?

When you’re shopping for antibodies, there are so many factors to consider. For example, will it wor...
Chris Sumner Jan 30, 2019

Hallmarks of Cancer: Resisting Cell Death

One thing we know about cancer cells: they can resist death. They evade apoptosis, the mechanism tha...
Chris Sumner Jan 23, 2019

Can I accelerate my ELISA results?

Running an ELISA can be a pain. Identifying pairs for an ELISA is a tedious business, and that’s bef...
Chris Sumner Jan 16, 2019

When should I use flow cytometry instead of western blot?

Western blotting is used to detect proteins and analyze their expression using antibodies, but it do...

Mycoplasma Cell Contamination: Tips for Prevention, Testing & Treatment

Any form of cell culture contamination can ruin your day and destroy your hard work, but mycoplasma ...
Michelle Graham Dec 5, 2018

Writing a Research Grant: Timelines, Future Directions, & Your Grant Title

Finally, the finish line is approaching! You have completed your specific aims, significance and inn...
Tamar Aprahamian, PhD Nov 7, 2018

Writing a Research Grant: The Experimental Approach

In the previous post on grant writing, we described how to write an effective significance and innov...
Tamar Aprahamian, PhD Oct 31, 2018

Writing a Research Grant: Explaining Significance & Innovation

The significance and innovation section is a recent (within the last 10 years) addition to the Natio...
Tamar Aprahamian, PhD Oct 24, 2018

Writing a Research Grant: Selecting a Target Award & Writing Specific Aims

So you’re thinking of writing a grant. Or maybe your mentor has politely suggested that it would be ...
Tamar Aprahamian, PhD Oct 17, 2018

Demystifying Antibody Panel Design for Multiplex IHC

In recent years, immune checkpoints in the tumor microenvironment have been under intense study. If ...
Jen Z Oct 10, 2018

ChIP & ChIP-seq: Should I Use Monoclonal or Polyclonal Antibodies?

Researchers who run a lot of chromatin immunoprecipitation ChIP assays—maybe even your advisor—might...
Kenneth Buck, PhD Sep 26, 2018

Research Trends in Macrophage Plasticity

It’s an exciting time for immuno-oncology research, as potential predictive biomarkers from an expan...
Kenneth Buck, PhD Sep 19, 2018

Milk or BSA? How to Choose a Blocking Protein for Western Blot [Video]

So you've set the timer to five minutes for the first of three TBST washes for your western blot mem...
Kenneth Buck, PhD Sep 5, 2018

Targeting B-Cell Maturation Antigen (BCMA) Holds Promise for Multiple Myeloma Treatment

Multiple myeloma (MM) is a type of hematopoietic malignancy characterized, in part, by bone pain, an...
Amrik Singh, PhD Aug 8, 2018

APOE4 & Insulin Receptor Signaling: A Hidden Link Between Diabetes & Alzheimer’s Disease?

You might be surprised to learn that diabetes, a metabolic disease, may be linked to the neurodegene...
Richard Cho, PhD Aug 1, 2018

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