CST BLOG: Lab Expectations

The official blog of Cell Signaling Technology® (CST) where we discuss what to expect from your time at the bench, share tips, tricks, and information.

Targeting TIGIT for Immunotherapy and T Cell Exhaustion

The evolution of solid tumor cancer treatment has historically centered on a tripartite approach tha...
Tamar A. Feb 17, 2021 3:00:00 AM

What is a Recombinant Antibody and Why is it Important?

Recombinant antibodies offer several key advantages compared to traditional antibodies. These includ...
Emma E Jul 15, 2020 3:00:00 AM

The Science Behind Sampler Kits

If one were asked to describe a Cell Signaling Technology (CST) antibody sampler kit, we would total...
Chris S Apr 29, 2020 3:00:00 AM

Behind the Antibodies - Interview with a Development Scientist

Have you ever wondered about the minds behind our antibodies? We talk a lot about validation, specif...
Chris S Oct 16, 2019 3:15:00 AM

What makes an antibody monoclonal, polyclonal, or recombinant?

When you’re shopping for antibodies, there are so many factors to consider. For example, will it wor...
Anthony C Jan 30, 2019 3:10:00 AM

Tech Tips Video: How much antibody should I use for ChIP?

It's time to check out another video from the CST Tech Tips playlist! In this edition of Tech Tips, ...
Ken B Nov 28, 2018 3:15:00 AM

Fluorescent Staining Using Multiple Antibodies

The use of multiple antibodies in a single experiment can provide useful information to researchers....
Tara W Sep 13, 2017 3:00:00 AM

Have You Ever Wondered: What's The Lowdown on Phospho-Specific Antibodies?

Early exploration of unmapped biological signaling pathways were carried out using radiolabeled phos...
Ken B Jul 26, 2017 3:20:00 AM

Successful Immunofluorescence: Antibody Dilution and Incubation Conditions

Part four of a series on immunofluorescence techniques. Check out previous posts on Validation, Expe...
Ken B May 10, 2017 3:01:00 AM

Successful Immunofluorescence: Experimental Controls

 Part two of a four-part series on Immunofluorescence. Check out our posts on Validation and Fixatio...
Ken B Mar 8, 2017 3:00:00 AM

Successful Immunofluorescence: The Importance of Validation

Part one of a four-part series on Immunofluorescence. Check out Experimental Controls and Fixation a...
Ken B Mar 1, 2017 3:00:00 AM

Simplifying Proteomics - Part 2

Part 1 gave an overview on mass spectrometry-based proteomics. Now it’s time to talk about how this ...
Claire S Feb 8, 2017 10:40:01 AM

Why High Concentration doesn't always mean good Antibody Performance.

If you buy primary antibodies from different places, you may have noticed that many companies list t...
Carolyn P Dec 14, 2016 3:00:00 AM

3 Questions to ask before starting your next immunofluorescence experiment.

Specificity, consistency, and optimized assay conditions are three key elements that help ensure rel...
Liana G Oct 19, 2016 3:00:00 AM

3 Questions to ask before starting your next immunofluorescence experiment.

Specificity, consistency, and optimized assay conditions are three key elements that help ensure rel...
Carolyn P Sep 7, 2016 3:00:00 AM

Roundtable Discussion on Antibody Validation

Antibody Validation Roundtable: Specificity + Sensitivity + Reproducibility = Validation  Concerns a...
Carolyn P Feb 17, 2016 3:00:00 AM

Simplifying Proteomics - Part 3

Part 1 and part 2 introduced mass spectrometry-based proteomic methods, like PTMScan®, for the study...
Claire S Dec 9, 2015 3:00:00 AM

Simplifying Proteomics - Part 2

Part 1 gave an overview on mass spectrometry-based proteomics. Now it’s time to talk about how this ...
Claire S Dec 2, 2015 3:00:00 AM

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