CST BLOG: Lab Expectations

The official blog of Cell Signaling Technology® (CST) where we discuss what to expect from your time at the bench, share tips, tricks, and information.

Lab Expectations listing page

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Comparing Antibody Concentrations & Performance; CST® Antibody Details You May Not Know

  When purchasing antibodies from Cell Signaling Technology (CST), you may notice that we typically ...
Alexandra Foley Oct 12, 2022

Tools to Initiate Discovery

You’re at an impasse. Maybe you’re embarking on a new research project, or your current project has ...
Chris Sumner Mar 16, 2022

When Specificity Is Simply Sweet

Rajan Burt, Samuel Myers, and colleagues from the La Jolla Institute for Immunology, Broad Institute...

Tools to Confirm Antibody Specificity

You want to generate accurate results. You’ve planned out your experiment, accounting for all necess...
Chris Sumner Mar 2, 2022

Tools for Screening and Quantifying Proteins

Whether your heart is devoted to cardiac research, you’re excited to investigate neurodegenerative d...
Steve M Oct 20, 2021

Targeting TIGIT for Immunotherapy and T Cell Exhaustion

The evolution of solid tumor cancer treatment has historically centered on a tripartite approach tha...
Tamar A Feb 17, 2021

What is a Recombinant Antibody and Why is it Important?

Recombinant antibodies offer several key advantages compared to traditional antibodies. These includ...
Emma E Jul 15, 2020

The Science Behind Sampler Kits

If one were asked to describe a Cell Signaling Technology (CST) antibody sampler kit, we would total...
Chris Sumner Apr 29, 2020

Behind the Antibodies - Interview with a Development Scientist

Have you ever wondered about the minds behind our antibodies? We talk a lot about validation, specif...
Chris Sumner Oct 16, 2019

What makes an antibody monoclonal, polyclonal, or recombinant?

When you’re shopping for antibodies, there are so many factors to consider. For example, will it wor...
Anthony Michetti Jan 30, 2019

Tech Tips Video: How much antibody should I use for ChIP?

It's time to check out another video from the CST Tech Tips playlist! In this edition of Tech Tips, ...
Kenneth Buck, PhD Nov 28, 2018

Have You Ever Wondered: What's The Lowdown on Phospho-Specific Antibodies?

Early exploration of unmapped biological signaling pathways were carried out using radiolabeled phos...
Kenneth Buck, PhD Jul 26, 2017

Successful Immunofluorescence: Antibody Dilution and Incubation Conditions

Part four of a series on immunofluorescence techniques. Check out previous posts on Validation, Expe...
Kenneth Buck, PhD May 10, 2017

Successful Immunofluorescence: Experimental Controls

 Part two of a four-part series on Immunofluorescence. Check out our posts on Validation and Fixatio...
Kenneth Buck, PhD Mar 8, 2017

Successful Immunofluorescence: The Importance of Validation

Part one of a four-part series on Immunofluorescence. Check out Experimental Controls, Fixation and ...
Kenneth Buck, PhD Mar 1, 2017

Immunofluorescence: The Questions to Ask Before Starting Your Next  Experiment

Specificity, consistency, and optimized assay conditions are three key elements that help ensure rel...
Liana G Oct 19, 2016

Three Questions to Ask Before Starting Your Next Immunofluorescence Experiment

Specificity, consistency, and optimized assay conditions are three key elements that help ensure rel...
Carolyn P Sep 7, 2016

Roundtable Discussion on Antibody Validation

Antibody Validation Roundtable: Specificity + Sensitivity + Reproducibility = Validation  Concerns a...
Carolyn P Feb 17, 2016

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