Pros and Cons of Different Multiplexing Techniques


Posted by Emma E on Jul 22, 2020 3:00:00 AM

Multiplex immunohistochemistry (mIHC) and multiplex immunofluorescence (mIF) are widely used to identify and localize different cell types within tissue samples. But choosing the right technique depends on the aims of the study and the tools available. A recent publication highlights the advantages and disadvantages of different approaches to mIHC/mIF and explains how these methods differ1.

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Topics: IHC, mIHC

Demystifying Multiplex IHC


Posted by Jen Z on Oct 10, 2018 3:15:00 AM

In recent years, immune checkpoint proteins in the tumor microenvironment have been under intense study. If you work in the immuno-oncology field, chances are you are either performing multiplex IHC (mIHC) or would like to. Ultimately, a multiplexed image like the one featured here provides a multi-layered depiction of a tumor, such that each antibody corresponds to a different fluorescent signal. If you want to detect more targets in your IHC, but aren’t sure how to design a panel of antibodies and fluorophores for mIHC, we’ll walk you through the process in this post.

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Topics: IHC, Cancer Research, mIHC, techniques, Immunology

Webinar - Multiplex Immunohistochemistry (IHC)


Posted by Liana G on Jun 8, 2016 3:00:00 AM

Analysis of Immune Checkpoint Control Protein Co-expression in Breast and Ovarian Cancer Using Novel Rabbit Monoclonal Antibodies and Multiplex IHC

With an increasing number of biomarkers and, often, limited availability of biopsy material, there is a growing need for multiplexed assays for both research and clinical purposes. IHC based solutions are particularly attractive in the field of immuno-oncology, as maintaining spatial context within the tumor microenvironment provides meaningful and potentially actionable information.

Watch the video below.

 

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Topics: Protocols, IHC, Cancer Immunology, Cancer Research, mIHC

Webinar - Multiplex Immunohistochemistry (IHC)


Posted by Liana G on Jan 13, 2016 3:00:00 AM

Analysis of Immune Checkpoint Control Protein Co-expression in Breast and Ovarian Cancer Using Novel Rabbit Monoclonal Antibodies and Multiplex IHC

With an increasing number of biomarkers and, often, limited availability of biopsy material, there is a growing need for multiplexed assays for both research and clinical purposes. IHC based solutions are particularly attractive in the field of immuno-oncology, as maintaining spatial context within the tumor microenvironment provides meaningful and potentially actionable information.

Watch the video below.

 

READ MORE >

Topics: Protocols, IHC, Cancer Immunology, Cancer Research, mIHC

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