CST BLOG: Lab Expectations

The official blog of Cell Signaling Technology® (CST) where we discuss what to expect from your time at the bench, share tips, tricks, and information.

Lab Expectations listing page

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Strategies for Successful Multiplex Immunofluorescence Experiments Using Conjugated Antibodies

One of the benefits of working with immunofluorescent (IF) analysis is the ability to compare multip...
Ginny Bain, PhD Apr 6, 2022

Tools for Classifying Cells and Identifying Protein Localization

When do I want to classify cells? Why do I care where a protein localizes? These are actually two di...
Andrea T Jan 26, 2022

How S6 Ribosomal Protein (5G10) Rabbit mAb Can Help You With Your Immunofluorescence Staining

One antibody is used in our lab so often that we each keep our own bulk stock! This antibody is S6 R...
John B Jan 27, 2021

Tissue Clearing from a Microscope Enthusiast’s Perspective

Biological imaging data has massive potential in terms of complexity of experiment and richness of d...
Ginny Bain, PhD Oct 30, 2019

Fluorescent Staining Using Multiple Antibodies: Two Common Techniques

The use of multiple antibodies in a single experiment can provide useful information to researchers....
Tara W Sep 13, 2017

Successful Immunofluorescence: Antibody Dilution and Incubation Conditions

Part four of a series on immunofluorescence techniques. Check out previous posts on Validation, Expe...
Kenneth Buck, PhD May 10, 2017

Successful Immunofluorescence: Fixation and Permeabilization

Part Three of a four-part series on immunofluorescence. Read Part 1: The Importance of Validation an...
Kenneth Buck, PhD Mar 15, 2017

Successful Immunofluorescence: Experimental Controls

 Part two of a four-part series on Immunofluorescence. Check out our posts on Validation and Fixatio...
Kenneth Buck, PhD Mar 8, 2017

Successful Immunofluorescence: The Importance of Validation

Part one of a four-part series on Immunofluorescence. Check out Experimental Controls, Fixation and ...
Kenneth Buck, PhD Mar 1, 2017

Immunofluorescence: The Questions to Ask Before Starting Your Next  Experiment

Specificity, consistency, and optimized assay conditions are three key elements that help ensure rel...
Liana G Oct 19, 2016

Three Questions to Ask Before Starting Your Next Immunofluorescence Experiment

Specificity, consistency, and optimized assay conditions are three key elements that help ensure rel...
Carolyn P Sep 7, 2016

5 Steps to Publication-Worthy IF Images

A picture is worth a thousand words, or in the case of immunofluorescent imaging, a thousand protein...
Tara W Sep 23, 2015

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