CST BLOG: Lab Expectations

The official blog of Cell Signaling Technology® (CST) where we discuss what to expect from your time at the bench, share tips, tricks, and information.

How S6 Ribosomal Protein (5G10) Rabbit mAb Can Help You With Your Immunofluorescence Staining

One antibody is used in our lab so often that we each keep our own bulk stock! This antibody is S6 R...
John B Jan 27, 2021 3:00:00 AM

Tissue Clearing from a Microscope Enthusiast’s Perspective

Biological imaging data has massive potential in terms of complexity of experiment and richness of d...
Ginny B. Oct 30, 2019 3:00:00 AM

Fluorescent Staining Using Multiple Antibodies

The use of multiple antibodies in a single experiment can provide useful information to researchers....
Tara W Sep 13, 2017 3:00:00 AM

Successful Immunofluorescence: Antibody Dilution and Incubation Conditions

Part four of a series on immunofluorescence techniques. Check out previous posts on Validation, Expe...
Ken B May 10, 2017 3:01:00 AM

Successful Immunofluorescence: Fixation and Permeabilization

Part Three of a four-part series on Immunofluorescence. Check out The Importance of Validation and E...
Ken B Mar 15, 2017 3:00:00 AM

Successful Immunofluorescence: Experimental Controls

 Part two of a four-part series on Immunofluorescence. Check out our posts on Validation and Fixatio...
Ken B Mar 8, 2017 3:00:00 AM

Successful Immunofluorescence: The Importance of Validation

Part one of a four-part series on Immunofluorescence. Check out Experimental Controls and Fixation a...
Ken B Mar 1, 2017 3:00:00 AM

3 Questions to ask before starting your next immunofluorescence experiment.

Specificity, consistency, and optimized assay conditions are three key elements that help ensure rel...
Liana G Oct 19, 2016 3:00:00 AM

3 Questions to ask before starting your next immunofluorescence experiment.

Specificity, consistency, and optimized assay conditions are three key elements that help ensure rel...
Carolyn P Sep 7, 2016 3:00:00 AM

5 Steps to Publication-Worthy IF Images

A picture is worth a thousand words, or in the case of immunofluorescent imaging, a thousand protein...
Tara W Sep 23, 2015 8:00:00 AM

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