CST BLOG: Lab Expectations

The official blog of Cell Signaling Technology (CST), where we discuss what to expect from your time at the bench, share tips, tricks, and information.

Lab Expectations listing page

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Writing a Grant Part 1: First Things First

So you’re thinking of writing a grant. Or maybe your mentor has politely suggested that it would be ...
Tamar A Oct 17, 2018

Demystifying Antibody Panel Design for Multiplex IHC

In recent years, immune checkpoint proteins in the tumor microenvironment have been under intense st...
Jen Z Oct 10, 2018

Milk or BSA? How to Choose a Blocking Protein for Western Blot [Video]

So you've set the timer to five minutes for the first of three TBST washes for your western blot mem...
Kenneth Buck, PhD Sep 5, 2018

Product Scientist Q&A: From Bench Scientist to Benchtop Support

I’m a Product Scientist at Cell Signaling Technology (CST). My typical work day no longer looks like...
Kaitlin M Aug 29, 2018

Painless Publication: How to Write a Conference Abstract

Our previous blog post, “Painless Publication: How to Write a Journal Abstract,” walked you through ...
Chris Sumner Jun 27, 2018

Painless Publication: How to Write a Journal Abstract

Have you ever sat frozen at the keyboard, facing the due date for your abstract and asking yourself ...
Chris Sumner May 9, 2018

Are you ready to transfer your protein? Wet vs. semi-dry transfer

You are all amped up to run a western blot to identify “your favorite protein.” The lysates have bee...
Neha G Apr 18, 2018

Western Blot Normalization: Am I setting up my loading controls correctly?

You’re gathering data from all your experiments and preparing to present to your advisor and thesis ...
Kenneth Buck, PhD Nov 1, 2017

Fluorescent Staining Using Multiple Antibodies: Two Common Techniques

The use of multiple antibodies in a single experiment can provide useful information to researchers....
Tara W Sep 13, 2017

Have You Ever Wondered: What's The Lowdown on Phospho-Specific Antibodies?

Early exploration of unmapped biological signaling pathways was carried out using radiolabeled phosp...
Kenneth Buck, PhD Jul 26, 2017

4 Steps to Better Immunohistochemistry: Steps 2, 3, and 4

This is part two of a two-part series on how to optimize your IHC protocols. Part one introduced the...
Carolyn P May 31, 2017

Four Steps to Better Immunohistochemistry: Step 1 - Antigen Retrieval

It’s Friday night and you could be out with your friends right now, but instead you’re tucked away i...
Carolyn P May 24, 2017

Successful Immunofluorescence: Fixation and Permeabilization

Part Three of a four-part series on successful immunofluorescence. Read Part 1: The Importance of Va...
Kenneth Buck, PhD Mar 15, 2017

The Potential of Proteomics: A Clear Path to Discovery | Part II

See a real life example of how PTMScan technology can facilitate translational discovery. In this sh...
Carolyn P Feb 22, 2017

The Potential of Proteomics: A Clear Path to Discovery

Whether your lab is well-versed and equipped to do Mass Spec analysis in-house or your lab has never...
Carolyn P Jan 11, 2017

Three Questions to Ask Before Starting Your Next Immunofluorescence Experiment

Specificity, consistency, and optimized assay conditions are three key elements that help ensure rel...
Carolyn P Sep 7, 2016

The Cost of a Failed Western Blot

Thinking about lab expenses isn’t as enjoyable as investigating your favorite signaling pathway. How...
Claire S Aug 13, 2015

Intracellular Flow Cytometry in Action

Traditionally, flow cytometry has been used to identify distinct cell types within a heterogeneous p...
Liana G Aug 12, 2015

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