Writing a Grant Part 2: Significance and Innovation


Posted by Tamar A. on Oct 24, 2018 7:43:37 AM

The significance and innovation section is a recent (within the last 10 years) addition to the NIH and most other foundation grant applications. It is a place for you to showcase WHY the work should be done – WHY there is a significant need for your study, and HOW the work is different from everyone else’s approach. What makes it groundbreaking, original research, work that will advance our scientific knowledge?

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Topics: techniques, Science Education

Writing a Grant Part 1: First Things First


Posted by Tamar A. on Oct 17, 2018 3:15:00 AM

So you’re thinking of writing a grant? Or maybe your mentor has politely suggested that it would be in your best interest to do so?

Where do you start?

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Topics: techniques, Career Development, Science Education

Demystifying Multiplex IHC


Posted by Jen Z on Oct 10, 2018 3:15:00 AM

In recent years, immune checkpoint proteins in the tumor microenvironment have been under intense study. If you work in the immuno-oncology field, chances are you are either performing multiplex IHC (mIHC) or would like to. Ultimately, a multiplexed image like the one featured here provides a multi-layered depiction of a tumor, such that each antibody corresponds to a different fluorescent signal. If you want to detect more targets in your IHC, but aren’t sure how to design a panel of antibodies and fluorophores for mIHC, we’ll walk you through the process in this post.

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Topics: IHC, Cancer Research, mIHC, techniques, Immunology

Tech Tips Video: Milk or BSA? Choosing a blocking protein for Western Blot


Posted by Ken B on Sep 5, 2018 3:00:00 AM

So you've set the timer to five minutes for the first of three TBST washes for your western blot membrane. Now what? Sure, you could check your email or social media for the 30th time before lunch. Or you could do something informative, like check out a CST Tech Tips video! This is a new short video series featuring the same scientists who develop and validate CST antibodies, here to offer insights and protocol tips.

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Topics: Protocols, Western Blot, techniques, Tech Tips

From Bench Scientist to Benchtop Support


Posted by Kaitlin M. on Aug 29, 2018 3:15:00 AM

I’m a Product Scientist at Cell Signaling Technology (CST). My typical work day no longer looks like my fellow scientists’ daily grind, and at times, I even find it difficult to describe in words what I do, but I’ll give it a shot!

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Topics: Antibody Performance, techniques, Science Education

Painless Publication: How to Write a Conference Abstract


Posted by Chris S on Jun 27, 2018 3:15:00 AM

Our previous blog post, “Painless Publication: How to Write a Journal Abstract,” walked you through the steps in writing the abstract for a journal article. Now we turn our focus to writing abstracts for conference proceedings. Although there are some similarities between these two types of abstracts, there are also some distinct considerations and approaches for conference abstracts.

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Topics: techniques, Q&A, Career Development, Science Education

Painless Publication: How to Write a Journal Abstract


Posted by Chris S on May 9, 2018 3:10:00 AM

Have you ever sat frozen at the keyboard, facing the due date for your abstract and asking yourself how you can condense all of this information into 200 words or less? If this scenario resonates with you, rest assured you are not alone. There is a learning curve for writing an effective abstract, and thankfully there are also some tips that will help you get started and finish strong.

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Topics: techniques, Q&A, Career Development, Science Education

Are you ready to transfer your protein? Wet vs. semi-dry transfer


Posted by Neha G on Apr 18, 2018 3:15:00 AM

 You are all amped up to run a western blot to identify “your favorite protein.” The lysates have been run and proteins separated by SDS-PAGE. Now it’s time to transfer proteins from the gel to the membrane, and you’re sitting wondering….wet or semi-dry?? Or maybe you are better prepared than I was as a graduate student and you already know your next step, in which case you are aware of the pros and cons of wet and semi-dry transfer.

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Topics: Protocols, Western Blot, techniques, Science Education

Keep ChIP’ing away.


Posted by Curtis D on Jan 31, 2018 6:00:00 AM

So your experiments and data are funneling you down an inescapable path. You need to show direct gene regulation by your protein of interest. You think to yourself, “Oh, ChIP...”

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Topics: Protocols, Antibody Performance, ChIP, techniques, Reproducility

Have you ever wondered: Am I setting up my loading controls correctly?


Posted by Ken B on Nov 1, 2017 3:00:00 AM

You’re gathering data from all your experiments and preparing to present to your advisor and thesis committee at your annual progress report. You have an interesting hypothesis, and you have a validated antibody that recognizes your target protein on a western blot (WB). The molecular weight of the band is correct, and the expression of the target protein changes just the way you predicted it would. Now, you know — and you’d bet the house on it — when that powerpoint slide comes up, someone on your committee is going to ask about loading controls.

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Topics: Protocols, Antibody Performance, Western Blot, Companion Reagents, techniques

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