Traditionally, flow cytometry has been used to identify distinct cell types within a heterogeneous pool of cells, based on extracellular or surface marker expression, an application commonly known as immuno-phenotyping. However, this technology is also readily amenable to intracellular target detection and can be successfully applied to the study of complex signaling events.
Thus, when combined with the detection of cell surface markers intracellular flow cytometry becomes a powerful platform, which enables characterization of signaling networks at a single cell level within phenotypically distinct cell populations.
Advantages of Intracellular Flow Cytometry:
- Multi-parameter (e.g., multiple intra- and extra-cellular targets, viability, DNA content, cytokine profile, etc.)
- Sensitivity (single cell analysis)
- Quantitative output
- High throughput (up to a 1000 cells/sec can be processed)
- Fast readout (min to hours to generate data)
Check out the video below to see how it works . . .
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