CST BLOG: Lab Expectations

The official blog of Cell Signaling Technology® (CST) where we discuss what to expect from your time at the bench, share tips, tricks, and information.

The Cost of a Failed Western Blot

Thinking about lab expenses isn’t as enjoyable as investigating your favorite signaling pathway. How...
Claire S Sep 14, 2016 3:00:00 AM

3 Questions to ask before starting your next immunofluorescence experiment.

Specificity, consistency, and optimized assay conditions are three key elements that help ensure rel...
Carolyn P Sep 7, 2016 3:00:00 AM

The Cost of a Failed Western Blot

Thinking about lab expenses isn’t as enjoyable as investigating your favorite signaling pathway. How...
Claire S Aug 13, 2015 8:13:02 PM

Intracellular Flow Cytometry in Action

Traditionally, flow cytometry has been used to identify distinct cell types within a heterogeneous p...
Liana G Aug 12, 2015 8:00:00 AM

Need Better ChIP results? Use Enzymatic Digestion

The success or failure of a chromatin immunprecipitation (ChIP) experiment is highly dependent on th...
Carolyn P Aug 5, 2015 12:33:00 PM

Why High Concentration doesn't always mean good Antibody Performance.

If you buy primary antibodies from different places, you may have noticed that many companies list t...
Carolyn P Jul 29, 2015 5:00:00 AM

4 Steps to Better Immunohistochemistry: Steps 2, 3, and 4.

This is part two of a two-part series on how to optimize your IHC protocols. Part one introduced the...
Carolyn P Jul 15, 2015 7:00:00 AM

4 Steps to Better Immunohistochemistry: Step 1 - Antigen Retrieval

It’s Friday night - you’re tucked away in a dark, little room filled with microscopes.You could be o...
Carolyn P Jul 8, 2015 7:00:00 AM

PTMScan Tutorial Video

Simplifying proteomics part 1 introduced mass spectrometry-based proteomics for profiling post-trans...
Claire S Jun 10, 2015 5:30:00 AM

4 Steps to Better ChIP results - Step 4 - Downstream Analysis

Part one of this series described the importance of including proper controls to your protocols. In ...
Carolyn P Mar 11, 2015 3:00:00 AM

4 Steps to Better ChIP results - Step 3- Choosing an Antibody

Part one of this series described the importance of including proper controls to your protocols. In ...
Carolyn P Mar 4, 2015 3:00:00 AM

4 Steps to Better ChIP results - Step 2- Prep well

Part one of this series described the importance of including proper controls to your protocols. Now...
Carolyn P Feb 25, 2015 3:00:00 AM

4 Steps to Better ChIP results - Step 1- Take control

Introduction You're a confocal maestro, playing the lasers and the filters and the gain settings lik...
Carolyn P Feb 18, 2015 3:00:00 AM
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