CST BLOG: Lab Expectations

The official blog of Cell Signaling Technology® (CST) where we discuss what to expect from your time at the bench, share tips, tricks, and information.

Video: How to Choose Enzymatic or Sonication Protocol for ChIP

The success of your chromatin immunoprecipitation (ChIP) experiment depends on the fragmentation of ...
Ken B Nov 6, 2019 3:00:00 AM

Tech Tips Video: How much antibody should I use for ChIP?

It's time to check out another video from the CST Tech Tips playlist! In this edition of Tech Tips, ...
Ken B Nov 28, 2018 3:15:00 AM

Tech Tips Video: Milk or BSA? Choosing a blocking protein for Western Blot

So you've set the timer to five minutes for the first of three TBST washes for your western blot mem...
Ken B Sep 5, 2018 3:00:00 AM

Mastering Phospho-Flow: Immune Cell Activation Webinar

To characterize activation of immune cells, measurement of intracellular signaling using phospho-spe...
Chris S Jul 11, 2018 3:15:00 AM

Are you ready to transfer your protein? Wet vs. semi-dry transfer

 You are all amped up to run a western blot to identify “your favorite protein.” The lysates have be...
Neha G Apr 18, 2018 3:15:00 AM

Keep ChIP’ing away.

So your experiments and data are funneling you down an inescapable path. You need to show direct gen...
Curtis D Jan 31, 2018 6:00:00 AM

Have you ever wondered: Am I setting up my loading controls correctly?

You’re gathering data from all your experiments and preparing to present to your advisor and thesis ...
Ken B Nov 1, 2017 3:00:00 AM

Fluorescent Staining Using Multiple Antibodies

The use of multiple antibodies in a single experiment can provide useful information to researchers....
Tara W Sep 13, 2017 3:00:00 AM

5 Steps to Publication-Worthy IF Images

A picture is worth a thousand words, or in the case of immunofluorescent imaging, a thousand protein...
Tara W Sep 6, 2017 3:30:00 PM

Switching from Manual to Automated IHC? We've got your back

If you’ve ever transitioned your IHC experiments from a manual protocol to an automated platform, yo...
Chris G Aug 30, 2017 3:00:00 AM

Intracellular Flow Cytometry in Action

Traditionally, flow cytometry has been used to identify distinct cell types within a heterogeneous p...
Liana G Aug 9, 2017 9:45:00 AM

4 Steps to Better Immunohistochemistry: Steps 2, 3, and 4

This is part two of a two-part series on how to optimize your IHC protocols. Part one introduced the...
Carolyn P May 31, 2017 3:00:00 AM

4 Steps to Better Immunohistochemistry: Step 1 - Antigen Retrieval

It’s Friday night and you could be out with your friends right now, but instead you’re tucked away i...
Carolyn P May 24, 2017 3:00:00 AM

Successful Immunofluorescence: Antibody Dilution and Incubation Conditions

Part four of a series on immunofluorescence techniques. Check out previous posts on Validation, Expe...
Ken B May 10, 2017 3:01:00 AM

Successful Immunofluorescence: Fixation and Permeabilization

Part Three of a four-part series on Immunofluorescence. Check out The Importance of Validation and E...
Ken B Mar 15, 2017 3:00:00 AM

Successful Immunofluorescence: Experimental Controls

 Part two of a four-part series on Immunofluorescence. Check out our posts on Validation and Fixatio...
Ken B Mar 8, 2017 3:00:00 AM

Successful Immunofluorescence: The Importance of Validation

Part one of a four-part series on Immunofluorescence. Check out Experimental Controls and Fixation a...
Ken B Mar 1, 2017 3:00:00 AM

3 Questions to ask before starting your next immunofluorescence experiment.

Specificity, consistency, and optimized assay conditions are three key elements that help ensure rel...
Liana G Oct 19, 2016 3:00:00 AM

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