Successful Immunofluorescence: The Importance of Validation

Posted by Ken B on Mar 1, 2017 3:00:00 AM

Part one of a four-part series on Immunofluorescence. Check out Experimental Controls and Fixation and Permeabilzation.

After months of hard work, your research has zeroed in on a hypothesis you can test with immunofluorescence (IF). But now you have to make a choice. How do you decide which antibody to use to get reliable IF results? How do you know if the images are accurately reporting the target's localization? We explore some considerations in this post.


Topics: Protocols, Antibody Performance, Primary Antibodies, Antibody Validation, IF-IC, Reproducility

Season's Greetings from CST!

Posted by Ken B on Dec 21, 2016 3:00:00 AM

The holiday season is traditionally marked by festive choral music, decorated cookies, and in recent years, ugly sweater contests. CST employees get to experience all three traditions at the same time at the company’s yearly Holiday Concert.


Topics: Just for fun

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