Nature Communications recently published a novel study from the laboratory of Daniel Figeys at the University of Ottowa, which begins to unravel the complexity of the microbiome of pediatric patients with Crohn’s disease or CD, which is a type of inflammatory bowel disease (IBD). It causes inflammation of your digestive tract, which can cause symptoms like abdominal pain, severe diarrhea, fatigue, weight loss, and malnutrition.
Case Study: Acetylated Lysine and Its Impact on Crohn’s Disease
Posted by Charles F and Josh N on Dec 16, 2020 3:00:00 AM
Studying Post-translationally Modified Sites and Disease Variants with PhosphoSitePlus®
Posted by Florian G. on Feb 13, 2019 3:10:00 AM
Post-translational modifications (PTMs) are employed in the cell to alter protein function, and typically involve small chemical changes on the surface of the protein. The dynamic enzyme-catalyzed process of adding or subtracting PTMs mediates signaling in the cell. In other words, PTMs enhance protein-protein communication, enabling the cell to respond to internal or external signals.
Topics: Post Translational Modification
Researchers who run a lot of chromatin immunoprecipitation "ChIP" assays – maybe even your advisor – might subscribe to the idea that polyclonal antibodies perform better than monoclonal antibodies. But is that always actually true?
It’s worth your time to understand the differences between the two in terms of antigen recognition and specificity, and dispel some myths.
Topics: Antibody Performance, ChIP, Antibody Validation, Post Translational Modification, Reproducibility
Have You Ever Wondered: What's The Lowdown on Phospho-Specific Antibodies?
Posted by Ken B on Jul 26, 2017 3:20:00 AM
Early exploration of unmapped biological signaling pathways were carried out using radiolabeled phospho-imaging. The development of phospho-specific antibodies to detect and quantify protein phosphorylation made life easier for researchers (less 32P waste to deal with), but the interpretation of data from these experiments comes with its own set of caveats.
Topics: Antibody Performance, Primary Antibodies, Western Blot, Post Translational Modification, techniques
Part two of a four-part series on Immunofluorescence. Check out our posts on Validation and Fixation and Permeabilization.
After months of hard work, your research has honed in on a hypothesis you can test with immunofluorescence (IF). You've chosen antibodies and performed pilot IF experiments (see The Importance of Validation), and the localization of the protein appears reasonable. But how can you be sure the IF data you've acquired represents real biological phenomena? We present two examples of experimental controls in this post.
Topics: Protocols, Antibody Performance, Primary Antibodies, Antibody Validation, IF-IC, Post Translational Modification, Reproducibility
Part 1 gave an overview on mass spectrometry-based proteomics. Now it’s time to talk about how this strategy can be used to identify peptides with post-translational modifications (PTM) from a complex biological sample.
Topics: Primary Antibodies, Post Translational Modification, Proteomics
After sequencing of the human genome was complete, it was time to roll up our sleeves and get started on the daunting task of unraveling the complexity of the proteome. Thus the era of proteomics, the study of the function of all expressed proteins, was born. This task is especially complicated
Topics: Primary Antibodies, Post Translational Modification, Proteomics
Part 1 gave an overview on mass spectrometry-based proteomics. Now it’s time to talk about how this strategy can be used to identify peptides with post-translational modifications (PTM) from a complex biological sample.
Topics: Primary Antibodies, Post Translational Modification, Proteomics
After sequencing of the human genome was complete, it was time to roll up our sleeves and get started on the daunting task of unraveling the complexity of the proteome. Thus the era of proteomics, the study of the function of all expressed proteins, was born. This task is especially complicated because unlike the human genome, which is largely static in every cell, the proteome is different between say a liver cell and a brain cell, or between a healthy cell and a cancerous cell, or even between an individual cell at the different stages of development. To address this challenge the Human Proteome Project was founded. Its mission is to characterize all human genes by generating a map of the protein based molecular architecture of the body. In this way, it will become a resource to help elucidate the biological and molecular function of genes and facilitate the advanced diagnosis and treatment of disease.