CST BLOG: Lab Expectations

The official blog of Cell Signaling Technology® (CST) where we discuss what to expect from your time at the bench, share tips, tricks, and information.

What makes an antibody monoclonal, polyclonal, or recombinant?

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What makes an antibody monoclonal, polyclonal, or recombinant?

When you’re shopping for antibodies, there are so many factors to consider. For example, will it wor...
Anthony C Jan 30, 2019 3:10:00 AM

Myth-busting for ChIP/ChIP-seq: Mono vs. Poly

Researchers who run a lot of chromatin immunoprecipitation "ChIP" assays – maybe even your advisor –...
Neha G and Ken B Sep 26, 2018 3:15:00 AM

From Bench Scientist to Benchtop Support

I’m a Product Scientist at Cell Signaling Technology (CST). My typical work day no longer looks like...
Kaitlin M. Aug 29, 2018 3:15:00 AM

Shipping Temperature and Antibody Performance: You Asked. CST Answers.

Here at CST, we frequently get questions about the proper shipping temperature for antibodies. So we...
Chris S May 16, 2018 3:15:00 AM

Antibody Company of the Year (2018)

We as scientists learn from each success and failure. Sometimes it takes many failures to achieve su...
Chris S Feb 7, 2018 6:15:00 AM

Keep ChIP’ing away.

So your experiments and data are funneling you down an inescapable path. You need to show direct gen...
Curtis D Jan 31, 2018 6:00:00 AM

Webinar | Achieving Reproducibility: Don't Let Antibodies Be a Variable in Your Experiment

The importance of antibodies as tools in scientific research studies cannot be understated, yet thes...
Chris S Jan 10, 2018 6:30:00 AM

Have you ever wondered: Am I setting up my loading controls correctly?

You’re gathering data from all your experiments and preparing to present to your advisor and thesis ...
Ken B Nov 1, 2017 3:00:00 AM

Fluorescent Staining Using Multiple Antibodies

The use of multiple antibodies in a single experiment can provide useful information to researchers....
Tara W Sep 13, 2017 3:00:00 AM

Switching from Manual to Automated IHC? We've got your back

If you’ve ever transitioned your IHC experiments from a manual protocol to an automated platform, yo...
Chris G Aug 30, 2017 3:00:00 AM

Have You Ever Wondered: What's The Lowdown on Phospho-Specific Antibodies?

Early exploration of unmapped biological signaling pathways were carried out using radiolabeled phos...
Ken B Jul 26, 2017 3:20:00 AM

Successful Immunofluorescence: Antibody Dilution and Incubation Conditions

Part four of a series on immunofluorescence techniques. Check out previous posts on Validation, Expe...
Ken B May 10, 2017 3:01:00 AM

Successful Immunofluorescence: Fixation and Permeabilization

Part Three of a four-part series on Immunofluorescence. Check out The Importance of Validation and E...
Ken B Mar 15, 2017 3:00:00 AM

Successful Immunofluorescence: Experimental Controls

 Part two of a four-part series on Immunofluorescence. Check out our posts on Validation and Fixatio...
Ken B Mar 8, 2017 3:00:00 AM

Successful Immunofluorescence: The Importance of Validation

Part one of a four-part series on Immunofluorescence. Check out Experimental Controls and Fixation a...
Ken B Mar 1, 2017 3:00:00 AM

How to Validate a Histone Modification Antibody

Antibodies targeted to histone modifications may bind non-specifically to similar, but off-target hi...
Carolyn P Feb 1, 2017 3:00:00 AM

Why High Concentration doesn't always mean good Antibody Performance.

If you buy primary antibodies from different places, you may have noticed that many companies list t...
Carolyn P Dec 14, 2016 3:00:00 AM

The Cost of a Failed Western Blot

Thinking about lab expenses isn’t as enjoyable as investigating your favorite signaling pathway. How...
Claire S Sep 14, 2016 3:00:00 AM
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