CST BLOG: Lab Expectations

The official blog of Cell Signaling Technology® (CST) where we discuss what to expect from your time at the bench, share tips, tricks, and information.

What makes an antibody monoclonal, polyclonal, or recombinant?

When you’re shopping for antibodies, there are so many factors to consider. For example, will it wor...
Anthony C Jan 30, 2019 3:10:00 AM

Myth-busting for ChIP/ChIP-seq: Mono vs. Poly

Researchers who run a lot of chromatin immunoprecipitation "ChIP" assays – maybe even your advisor –...
Neha G and Ken B Sep 26, 2018 3:15:00 AM

From Bench Scientist to Benchtop Support

I’m a Product Scientist at Cell Signaling Technology (CST). My typical work day no longer looks like...
Kaitlin M. Aug 29, 2018 3:15:00 AM

Shipping Temperature and Antibody Performance: You Asked. CST Answers.

Here at CST, we frequently get questions about the proper shipping temperature for antibodies. So we...
Chris S May 16, 2018 3:15:00 AM

Antibody Company of the Year (2018)

We as scientists learn from each success and failure. Sometimes it takes many failures to achieve su...
Chris S Feb 7, 2018 6:15:00 AM

Keep ChIP’ing away.

So your experiments and data are funneling you down an inescapable path. You need to show direct gen...
Curtis D Jan 31, 2018 6:00:00 AM

Webinar | Achieving Reproducibility: Don't Let Antibodies Be a Variable in Your Experiment

The importance of antibodies as tools in scientific research studies cannot be understated, yet thes...
Chris S Jan 10, 2018 6:30:00 AM

Have you ever wondered: Am I setting up my loading controls correctly?

You’re gathering data from all your experiments and preparing to present to your advisor and thesis ...
Ken B Nov 1, 2017 3:00:00 AM

Fluorescent Staining Using Multiple Antibodies

The use of multiple antibodies in a single experiment can provide useful information to researchers....
Tara W Sep 13, 2017 3:00:00 AM

Switching from Manual to Automated IHC? We've got your back

If you’ve ever transitioned your IHC experiments from a manual protocol to an automated platform, yo...
Chris G Aug 30, 2017 3:00:00 AM

Have You Ever Wondered: What's The Lowdown on Phospho-Specific Antibodies?

Early exploration of unmapped biological signaling pathways were carried out using radiolabeled phos...
Ken B Jul 26, 2017 3:20:00 AM

Successful Immunofluorescence: Antibody Dilution and Incubation Conditions

Part four of a series on immunofluorescence techniques. Check out previous posts on Validation, Expe...
Ken B May 10, 2017 3:01:00 AM

Successful Immunofluorescence: Fixation and Permeabilization

Part Three of a four-part series on Immunofluorescence. Check out The Importance of Validation and E...
Ken B Mar 15, 2017 3:00:00 AM

Successful Immunofluorescence: Experimental Controls

 Part two of a four-part series on Immunofluorescence. Check out our posts on Validation and Fixatio...
Ken B Mar 8, 2017 3:00:00 AM

Successful Immunofluorescence: The Importance of Validation

Part one of a four-part series on Immunofluorescence. Check out Experimental Controls and Fixation a...
Ken B Mar 1, 2017 3:00:00 AM

How to Validate a Histone Modification Antibody

Antibodies targeted to histone modifications may bind non-specifically to similar, but off-target hi...
Carolyn P Feb 1, 2017 3:00:00 AM

Why High Concentration doesn't always mean good Antibody Performance.

If you buy primary antibodies from different places, you may have noticed that many companies list t...
Carolyn P Dec 14, 2016 3:00:00 AM

The Cost of a Failed Western Blot

Thinking about lab expenses isn’t as enjoyable as investigating your favorite signaling pathway. How...
Claire S Sep 14, 2016 3:00:00 AM
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