CST BLOG: Lab Expectations

The official blog of Cell Signaling Technology (CST), where we discuss what to expect from your time at the bench, share tips, tricks, and information.

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What makes an antibody monoclonal, polyclonal, or recombinant?

When you’re shopping for antibodies, there are so many factors to consider. For example, will it wor...
Chris Sumner Jan 30, 2019

Myth-busting for ChIP/ChIP-seq: Using Mono vs Polyclonal Antibodies

Researchers who run a lot of chromatin immunoprecipitation "ChIP" assays—maybe even your advisor—mig...
Kenneth Buck, PhD Sep 26, 2018

Product Scientist Q&A: From Bench Scientist to Benchtop Support

I’m a Product Scientist at Cell Signaling Technology (CST). My typical work day no longer looks like...
Kaitlin M Aug 29, 2018

Shipping Temperature and Antibody Performance: You Asked. CST Answers.

Here at CST, we frequently get questions about the proper shipping temperature for antibodies. So we...
Chris Sumner May 16, 2018

Antibody Company of the Year (2018)

We as scientists learn from each success and failure. Sometimes it takes many failures to achieve su...
Chris Sumner Feb 7, 2018

Keep ChIP’ing Away

So your experiments and data are funneling you down an inescapable path. You need to show direct gen...
Curtis Desilets Jan 31, 2018

Webinar | Achieving Reproducibility: Don't Let Antibodies Be a Variable in Your Experiment

The importance of antibodies as tools in scientific research studies cannot be understated, yet thes...
Chris Sumner Jan 10, 2018

Have You Ever Wondered: What's The Lowdown on Phospho-Specific Antibodies?

Early exploration of unmapped biological signaling pathways was carried out using radiolabeled phosp...
Kenneth Buck, PhD Jul 26, 2017

Successful Immunofluorescence: Antibody Dilution & Incubation Conditions

Part four of a four-part series on successful immunofluorescence. Read Part 1: The Importance of Val...
Kenneth Buck, PhD May 10, 2017

Successful Immunofluorescence: Fixation and Permeabilization

Part Three of a four-part series on successful immunofluorescence. Read Part 1: The Importance of Va...
Kenneth Buck, PhD Mar 15, 2017

How to Validate a Histone Modification Antibody

Antibodies targeted to histone modifications may bind non-specifically to similar, but off-target hi...
Carolyn P Feb 1, 2017

Roundtable Discussion on Antibody Validation

Antibody Validation Roundtable: Specificity + Sensitivity + Reproducibility = Validation Concerns ab...
Carolyn P Feb 17, 2016

Five Steps to Publication-Worthy IF Images

A picture is worth a thousand words, or in the case of immunofluorescent (IF) imaging, a thousand pr...
Tara W Sep 23, 2015

The Cost of a Failed Western Blot

Thinking about lab expenses isn’t as enjoyable as investigating your favorite signaling pathway. How...
Claire S Aug 13, 2015

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