Not all cancer cells are equal, they evolve in response to selective pressure driven by accumulation of mutations. Cancer cells have to out-compete nearby cells for nutrients and other resources, avoid immune cell attack, and suppress apoptotic self-destruction.
Cancer cells hijack inflammatory mechanisms to promote their own growth and survival. During a normal inflammatory response by the innate and adaptive immune system, immune cells carry out their designated task of engulfing and/or destroying foreign invaders.
Gain insight into the processes by which senescent cells contribute to tumor suppression and age-related pathologies. This webinar explores the impact of senescence on age-related dysfunction and chronic disease and introduces potential therapies targeting senescent cells.
Careful planning and the fine tuning of experimental protocols are key to ensuring clear, interpretable scientific results. This is especially true for immunohistochemistry (IHC) studies, where each step in the often multiday process – from tissue preparation to stain development – can significantly impact the final outcome and analysis. Often, the simultaneous examination of multiple antigens is required to address specific scientific questions, which further complicates IHC protocol development. A general understating of the steps necessary to optimize IHC for multiple targets is essential to achieve reliable results. So, what are these steps?
Parkinson’s disease (PD) is a neurodegenerative disease marked by loss of dopaminergic neurons in the substantia nigra. Mutations in the gene that encodes a ubiquitin ligase PARK2/Parkin are known to cause autosomal recessive forms of familial PD1. Parkin plays a key role in mitochondrial homeostasis by regulating a specialized form of autophagy called mitophagy, the clearance of defective or damaged mitochondria by lysosomes2.
How does altered mitochondrial homeostasis contribute to PD?
The massive amount of DNA in the human body is truly baffling. Stretched end to end, the DNA from a single somatic cell is about 2 meters in length, doing the same for all the DNA in the average human would reach the end of the solar system and back!
Cancer cells invade local tissue and spread to distant sites via two distinct, but similar processes known as invasion and metastasis.
Our immune system has the ability to detect and fight infectious pathogens. It can also detect when normal cells become cancerous and kill those cells, preventing cancer progression. But over time, cancers can evolve and evade the immune response.
Researchers use chromatin immunoprecipitation, or ChIP, to identify and characterize protein-DNA interactions in the context of chromatin. ChIP experiments can use varying input samples, chromatin fragmentation methods, and provide ChIP-qPCR or ChIP-seq readouts.
Immunohistochemistry, or IHC, remains the simplest method for detecting biomarker expression while maintaining spatial context within tissues. You know that getting reliable IHC staining results hinges on the specificity and performance of your antibody. These are high stakes experiments, and you want to be 100% confident your antibody will detect the target of interest.
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