Introduction to the Hallmarks of Antibody Validation


Posted by Chris S on Jan 15, 2020 3:00:00 AM

Antibodies are essential reagents that support all levels of scientific research. Used in a multitude of applications to identify, quantify, and isolate specific target biomolecules, they have recently become the focus of intense scrutiny for their contribution to the ongoing reproducibility crisis.

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Topics: Antibody Performance, Antibody Validation

Immunology Overview: How does our immune system protect us?


Posted by April L on Jan 8, 2020 3:15:00 AM

Scientists and medical professionals have been studying what makes us sick and the myriad mechanisms the body uses to respond to illness for hundreds of years. Some of these mechanisms are quite simple and some are elegantly complex. In this first installment of the Immunology blog series we will review the different strategies the immune system employs to keep diseases at bay.

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Topics: Immunology

Coming to Lab Expectations in 2020


Posted by Chris S on Jan 1, 2020 3:15:00 AM

            The start of a new year is always a crazy time. There’s plenty to look back on and celebrate, things to anticipate, and of course there are opportunities for improvement. What do we look forward to in 2020? We’re about to celebrate our 5th blog anniversary! It’s hard to believe that Lab Expectations is five years old.

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Topics: Science Education

4 Situations Where Flow Cytometry Might Be More Useful Than Western Blotting


Posted by Chris S on Dec 4, 2019 3:00:00 AM

Western blotting is used to detect proteins and analyze their expression using antibodies, but it does have its limitations. Flow cytometry also employs antibodies, but it uses fluorescence to detect and analyze protein expression on a cell-by-cell basis.

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Topics: Western Blot, Flow, techniques

Critical Considerations for Optimizing Imaging Mass Cytometry


Posted by Chris S on Nov 20, 2019 3:15:00 AM

Understanding the microenvironment of disease is critical for the success of targeted therapies. For years, the complex landscape of the tumor microenvironment could only be studied in single-cell snapshots by flow cytometry or limited tissue staining. Advanced multiplexing technologies such as Imaging Mass Cytometry™ (IMC™) now enable the imaging of up to 40 protein markers on both the cellular and tissue level.

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Topics: IHC

Video: How to Choose Enzymatic or Sonication Protocol for ChIP


Posted by Ken B on Nov 6, 2019 3:00:00 AM

The success of your chromatin immunoprecipitation (ChIP) experiment depends on the fragmentation of chromatin, a critical step in the ChIP protocol. This can be accomplished with either sonication or enzymatic digestion. But how do you decide which chromatin fragmentation protocol to use in your ChIP experiments? A number of factors can influence your choice, making a decision seem daunting. So let’s simplify – watch the video and we'll will show you the way!

 

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Topics: Protocols, ChIP, techniques

Tissue Clearing from a Microscope Enthusiast’s Perspective


Posted by Ginny B. on Oct 30, 2019 3:00:00 AM

Biological imaging data has massive potential in terms of complexity of experiment and richness of dataset. While early imaging experiments were mostly descriptive, showing if "target X presents in cell of interest;" modern experiments have the potential to massively multiplex, define complex spatial relationships, measure levels or numbers of tiny subcellular components, and even measure mRNA.

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Topics: IF-IC, Neuroscience

Imaging Mass Cytometry: Endless possibilities to understand the microenvironment of disease


Posted by Kris H on Oct 23, 2019 3:15:00 AM

Understanding the microenvironment of disease is critical. Targeted cancer therapies may not be effective depending on the combination of cell types in the tumor. Likewise, an immune cell invasion in pancreatic tissue could indicate the progression of type 1 diabetes. For years, this complex landscape could only be witnessed in single-cell snapshots by flow cytometry or limited tissue staining. Imaging Mass Cytometry™ (IMC™) now offers a panorama of this dynamic world.

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Topics: IHC, techniques

Behind the Antibodies - Interview with a Development Scientist


Posted by Chris S on Oct 16, 2019 3:15:00 AM

Have you ever wondered about the minds behind our antibodies? We talk a lot about validation, specificity, sensitivity, and reproducibility. All of that is very important, but that doesn't tell you much about who developed it.

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Topics: Antibody Performance, Primary Antibodies, Antibody Validation

The Guide to the Hallmarks of Cancer Research Targets


Posted by Chris S on Oct 9, 2019 3:15:00 AM

If you haven’t heard already, it’s CST’s 20th anniversary. Woohoo! You could say this year is kind of a big deal for us. We have a lot to celebrate. However, once we started listing the successes over the years, we realized that we are most proud of what you – the researchers worldwide – have accomplished using our reagents. After all, we don’t live in a vacuum. We exist to serve you and enable important discoveries and the development of cures.

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Topics: Cell Biology, Cancer Research

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