Journal Club: TREM2 Opens a Rabbit Hole of Questions for Alzheimer’s Researchers

Posted by Richard C on Nov 8, 2017 3:00:00 AM

It's almost time for the 2017 Society for Neuroscience meeting. To get your neurons excited for the meeting, here's a journal club discussing a recent paper with interesting findings for Alzheimer's disease. 

The pathological hallmark of Alzheimer’s disease (AD) is the accumulation of amyloid β (Aβ) plaques and neurofibrillary tangles. Despite decades of research, the direct (and indirect) contribution of these lesions in disease progression is poorly understood. Do these lesions directly cause neuronal dysfunction and neurodegeneration? If so, why do some patients accumulate these lesions, but exhibit normal neurological behavioral before death? Do AD patients have secondary defects in cellular and/or molecular processes that normally function to protect patients from accumulation of these nefarious lesions? If so, what are these cell types and what…


Take a breath.


Topics: Metabolism, Autophagy, Journal Club, Neuroscience, Alzheimer's Disease, Neurodegeneration

Have you ever wondered: Am I setting up my loading controls correctly?

Posted by Ken B on Nov 1, 2017 3:00:00 AM

You’re gathering data from all your experiments and preparing to present to your advisor and thesis committee at your annual progress report. You have an interesting hypothesis, and you have a validated antibody that recognizes your target protein on a western blot (WB). The molecular weight of the band is correct, and the expression of the target protein changes just the way you predicted it would. Now, you know — and you’d bet the house on it — when that powerpoint slide comes up, someone on your committee is going to ask about loading controls.


Topics: Protocols, Antibody Performance, Western Blot, Companion Reagents, techniques

What Assay Should You Be For Halloween?

Posted by Kira H on Oct 25, 2017 6:00:00 AM

In this time of ghosts, ghouls, and other scary things, we got to thinking about how we would respond in the event of a zombie apocalypse (ok, we may have watched a few too many scary movies while waiting for the confocal). Would you grab medicine and food for sustenance? Maybe you think offense is better than defense and go straight for the weapons … Or, perhaps you run immediately for the lab to maintain your 6-year old (and counting) thesis experiment?


Topics: Just for fun

Targeting Cancer Pathways - Tumor Resistance

Posted by Carolyn P on Oct 18, 2017 6:00:00 AM

Cell Signaling Technology is proud to partner with the Koch Institute at MITScience, and Science Signaling to present the Targeting Cancer Pathways webinar series. These webinars bring together thought leaders from around the world to share current findings and further cancer research community collaboration.


Topics: Cancer Research, Webinars

Organic Ideas for Composting at CST

Posted by Elias W on Oct 11, 2017 6:00:00 AM

As a company that believes in leaving our mark on science, but not on the earth, CST has made a commitment to use compostable cups and plates in our US cafeterias.


Topics: Corporate Social Responsibility

Webinar - Immunolabeling for Neurodegenerative Disease

Posted by Carolyn P on Oct 4, 2017 3:00:00 AM

This two part webinar features Dr. Li Huei Tsai from the Picower Institute at MIT and CST Development Scientist, Dr. Raphael Rozenfeld. Dr. Tsai discusses how modeling systems can be combined with immunolabeling and imaging techniques to unlock the mystery of Alzheimer's Disease. Dr Rozenfeld describes the rigorous validation procedure our antibodies undergo before being release, with special emphasis placed on our neuroscience portfolio. Check out the abstracts below and then click on the button to watch their exciting presentations.


Topics: Cancer Research

Autophagy: It’s a cell eat self world

Posted by Claire S on Sep 27, 2017 3:00:00 AM


If the thought of self-cannibalization is not appealing to you, you may not want to read the next sentence . . . Your cells are literally eating themselves right now! In the 1960s, Christian de Duve named this process “autophagy” from the Greek auto (self) and phagein (to eat). He was later awarded the Nobel Prize for Physiology or Medicine for his contribution to the cell biology field. Little did de Duve know what an issue this word would cause: do you pronounce it “aw-tof-a-gee” or, “auto-fay-gee”? Well, actually – it’s a bit like tom-ay-tow/tom-ah-tow – both are right! As de Duve himself said, “I coined the word, but not the pronunciation.”


Topics: Journal Club

Journal Club:  Hippo pathway and intestinal stem cell regeneration

Posted by Liana G on Sep 20, 2017 3:00:00 AM

Journal Club: YAP-dependent reprogramming of Lgr5+ stem cells drives intestinal regeneration and cancer.

The article we have chosen was published in the October 29th, 2015 issue of the journal Nature by Gregorieff et al., from the Lunenfeld Tanenbaum Research Institute and University of Toronto, Ontario Canada. The article is entitled YAP-dependent reprogramming of Lgr5+ stem cells drives intestinal regeneration and cancer, and focuses on stem cell dynamics within the intestinal epithelium, the most rapidly self-renewing tissue in adult mammals.


Topics: Cancer Research, Journal Club

Fluorescent Staining Using Multiple Antibodies

Posted by Tara W on Sep 13, 2017 3:00:00 AM

The use of multiple antibodies in a single experiment can provide useful information to researchers. Co-staining with multiple antibodies and cellular dyes is a simple, low-content form of multiplex analysis. Techniques for performing multiplex analyses in cells and tissues are powerful research tools that are applicable to general cell biology studies as well as diagnostic purposes. These techniques allow researchers to detect multiple biomarkers to assess their samples. They also allow for easy colocalization studies to determine relationships between analytes. Here we describe two common techniques for fluorescent staining using multiple antibodies in the same assay.


Topics: Protocols, Antibody Performance, Primary Antibodies, IF-IC, Companion Reagents, techniques

5 Steps to Publication-Worthy IF Images

Posted by Tara W on Sep 6, 2017 3:30:00 PM

A picture is worth a thousand words, or in the case of immunofluorescent imaging, a thousand proteins. The images used to illustrate a scientific experiment should convey as much information as the text itself. Here at CST, we pride ourselves in the quality of our antibodies and our rigorous validation process. When we approve our primary antibodies for IF, we like to showcase them using high quality images generated in-house. Beyond our recommended IF protocols (check it out here), here are some additional considerations to make when planning your IF staining. 


Topics: Protocols, Antibody Performance, Antibody Validation, IF-IC, techniques