Immunology: Can we re-train our T cells to attack cancer?


Posted by Tamar A. on Jun 3, 2020 3:00:00 AM

The human immune system comprises an array of ingenious cellular components and mechanisms that collectively protect us from harmful exogenous pathogens. Harnessing this power to attack and treat cancer is an intense area of research. Understanding why cancer cells normally evade immune surveillance and developing strategies to help the immune system distinguish between tumor cells and healthy tissue may lead to the development of highly efficacious therapies for those afflicted with cancer.

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Topics: Immunology

Immunology: What cells have a myeloid lineage and how are they identified?


Posted by Tamar A. on May 27, 2020 3:00:00 AM

During haematopoiesis in humans, cells of the myeloid lineage are derived from a common myeloid progenitor (CMP) in the bone marrow. This lineage — which includes monocytes, granulocytes, erythrocytes, and platelets — is a primary component of the innate immune system and serves as a first line of defense against infection.

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Topics: Immunology

9B11 Is a Superior Clone against Top-cited Myc-tag Competitor


Posted by Tamar A. on May 20, 2020 3:15:00 AM

Biological research is about expanding knowledge, so it's not uncommon for scientists to become interested in studying proteins for which antibodies have not yet been developed. How then, can scientists pursue mechanistic studies of these novel or less-characterized proteins? Epitope tagging is a powerful tool for these protein studies and is used in myriad experimental applications. However, care must be taken not only in choice of epitope tag, but in selection and validation of antibodies.

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Topics: Antibody Performance

10 Must-have Markers for Senescence Research


Posted by Tamar A. on May 13, 2020 3:00:00 AM

Interested in studying senescence? Understanding when and why cell cycle arrest occurs is critical to many fields of research, including (but not limited to) studies of development, aging, and cancer. We all know the best tools produce the best results, so make sure you have all your bases covered with this list of the top 10 targets for your senescence research!

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The Importance of Research Antibody Specificity and Reproducibility During the SARS-CoV-2 Crisis


Posted by Srikanth S on May 6, 2020 3:38:00 PM

During this time of crisis, healthcare providers and researchers around the world are racing to develop SARS-CoV-2 testing methods. Testing strategies include PCR to diagnose active infection (by testing for the presence of viral genetic material) and the use of antibody test kits on blood (also known as serological tests), in order to track exposure and possible immunity in the population (1,2,3). With these diagnostic tools, the scientific community aims to get a better understanding of the scale and trajectory of the SARS-CoV-2 pandemic.

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Topics: Antibody Performance, Antibody Validation, Reproducibility

The Science Behind Sampler Kits


Posted by Chris S on Apr 29, 2020 3:00:00 AM

If one were asked to describe a Cell Signaling Technology (CST) antibody sampler kit, we would totally understand an answer like, “it’s an assortment of antibodies.” Honestly, on its face, that answer isn’t exactly wrong. It just makes the process seem a little random. Especially considering the reality:

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Topics: Antibody Performance, Primary Antibodies

Cell Process - How is cell proliferation quantified?


Posted by Tamar A. on Apr 22, 2020 3:00:00 AM

The production of new cells through cellular proliferation impacts the development, growth, and maintenance of all tissues in the body. This process must be tightly regulated, since uncontrolled cell division – as seen in various cancers – can lead to tumor formation and disrupt organ function. These broad implications for biological activities highlight the importance of understanding and accurately measuring cellular proliferation in a variety of contexts.

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Topics: Cell Biology

Cell Process: What is cell viability and how can it be measured?


Posted by Tamar A. on Apr 15, 2020 3:00:00 AM

The health of cells in culture is critical to the success of your experiments. Have you ever been excited about the experimental results of a knock-down, drug treatment, or culture condition, only to realize later that the effects are skewed due to the amount of cell death that occurred in your samples? Measuring and comparing cell viability in your assays is important, whether it’s the data you’re pursuing or an important control in your experiment.

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Topics: Cell Biology

Hallmarks of Validation - Complementary Strategies


Posted by Katie C on Apr 8, 2020 3:00:00 AM

Depending on the antigenic target being studied and the application being used, it may be advisable to employ complementary strategies during antibody validation. These approaches can provide vital information regarding antibody specificity or functionality and can be carefully tailored to the biological nature of the target as well as to the exacting requirements of the downstream assay.

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Topics: Antibody Performance, Antibody Validation

Hallmarks of Validation - Heterologous Strategy


Posted by Katie C on Apr 1, 2020 3:00:00 AM

For antigenic targets where expression of the protein is very low or unknown, the use of recombinant proteins or exogenous expression in a surrogate cell line may be necessary for antibody validation. Although endogenous systems are preferred for their closer representation of in vivo conditions, heterologous strategy offers several advantages.

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Topics: Antibody Performance, Antibody Validation

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