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The official blog of Cell Signaling Technology, where we discuss what to expect from your time at the bench, share tips, tricks, and information.

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Antibody Essentials: Antibody Diversity and Classification

Understanding how antibodies are made and the pros and cons of the different types of antibodies is ...
Kenneth Buck, PhD Jul 14, 2021

Simplify iPSCs Research: Antibodies to Identify Pluripotency Markers

Reagent validation and experiment reproducibility are critical for advancing research in any scienti...
Chris Sumner Sep 30, 2020

Antibody Selection for Epitope Tagging: Insights from Myc-Tag Performance

Biological research is about expanding knowledge, so it's not uncommon for scientists to study prote...
Tamar Aprahamian, PhD May 20, 2020

Hallmarks of Validation - Complementary Strategies

Depending on the antigenic target being studied and the application being used, it may be advisable ...
Katie Crosby Apr 8, 2020

Hallmarks of Antibody Validation: Heterologous Strategy

For antigenic targets where expression of the protein is very low or unknown, the use of recombinant...
Katie Crosby Apr 1, 2020

Hallmarks of Validation - Multiple Antibody Strategy

A multiple antibody strategy is a powerful approach to antibody validation. One of the most common m...
Katie Crosby Mar 18, 2020

CiteAb Awards: Antibody Supplier of the Decade & Antibody Supplier Succeeding in Alzheimer's Research

We’re honored to win two CiteAb awards in 2020: Antibody Supplier of the Decade and Antibody Supplie...
Chris Sumner Mar 11, 2020

Hallmarks of Validation - Ranged Strategy

While a binary approach is one of the more desirable ways to evaluate antibody specificity, binary m...
Katie Crosby Feb 19, 2020

Hallmarks of Antibody Validation - Binary Strategy

A binary approach is one of the best ways to evaluate antibody specificity. By testing an antibody i...
Katie Crosby Jan 22, 2020

What makes an antibody monoclonal, polyclonal, or recombinant?

When you’re shopping for antibodies, there are so many factors to consider. For example, will it wor...
Chris Sumner Jan 30, 2019

Successful Immunofluorescence: The Importance of Validation

Part 1 of a four-part series on successful immunofluorescence. Read Part 2: Experimental Controls, P...
Kenneth Buck, PhD Mar 1, 2017

The Cost of a Failed Western Blot

Thinking about lab expenses isn’t as enjoyable as investigating your favorite signaling pathway. How...
Claire S Aug 13, 2015

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