Mastering Phospho-Flow: Immune Cell Activation Webinar


Posted by Chris S on Jul 11, 2018 3:15:00 AM

To characterize activation of immune cells, measurement of intracellular signaling using phospho-specific antibodies combined with immune phenotyping using surface markers is ideal. Owing to the unique capability of flow cytometry to measure events at a single-cell level, “Phospho Flow” allows for the identification of rare signaling events during processes such as co-stimulatory signaling and manipulation of checkpoint inhibition. However, combining extracellular and intracellular antibodies in a flow protocol can present technical challenges. 
 
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Topics: Flow, Protocols

Intracellular Flow Cytometry in Action


Posted by Liana G on Aug 9, 2017 9:45:00 AM


Traditionally, flow cytometry has been used to identify distinct cell types within a heterogeneous pool of cells, based on extracellular or surface marker expression, an application commonly known as immuno-phenotyping. However, this technology is also readily amenable to intracellular target detection and can be successfully applied to the study of complex signaling events.

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Topics: Protocols, Flow, Cell Biology, techniques

Intracellular Flow Cytometry in Action


Posted by Liana G on Aug 12, 2015 8:00:00 AM


Traditionally, flow cytometry has been used to identify distinct cell types within a heterogeneous pool of cells, based on extracellular or surface marker expression, an application commonly known as immuno-phenotyping. However, this technology is also readily amenable to intracellular target detection and can be successfully applied to the study of complex signaling events.

READ MORE >

Topics: Protocols, Flow, Cell Biology, techniques

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