Western blotting is used to detect proteins and analyze their expression using antibodies, but it does have its limitations. Flow cytometry also employs antibodies, but it uses fluorescence to detect and analyze protein expression on a cell-by-cell basis.
4 Situations Where Flow Cytometry Might Be More Useful Than Western Blotting
Posted by Chris S on Dec 4, 2019 3:00:00 AM
Topics: Western Blot, Flow, techniques
What is flow cytometry and how is it used?
Flow cytometry enables you to save time and analyze many characteristics of your cells in one experiment, using classic principles of antibody detection.
Topics: Flow
When should I use flow cytometry for signaling instead of western blot?
Posted by Chris S on Jan 9, 2019 3:15:00 AM
Flow cytometry enables quantitative analysis of protein expression, signaling states, and physical characteristics (cell size/granularity) at the single-cell level. Modern flow cytometers are capable of collecting data on multiple proteins from thousands of cells per second in a heterogeneous mixture. While flow cytometry is commonly employed to identify cell types using phenotypic markers expressed on the cell surface, it can also be used to measure intracellular signaling events.
Topics: Flow, techniques
Traditionally, flow cytometry has been used to identify distinct cell types within a heterogeneous pool of cells, based on extracellular or surface marker expression, an application commonly known as immuno-phenotyping. However, this technology is also readily amenable to intracellular target detection and can be successfully applied to the study of complex signaling events.
Topics: Protocols, Flow, Cell Biology, techniques
Traditionally, flow cytometry has been used to identify distinct cell types within a heterogeneous pool of cells, based on extracellular or surface marker expression, an application commonly known as immuno-phenotyping. However, this technology is also readily amenable to intracellular target detection and can be successfully applied to the study of complex signaling events.
Topics: Protocols, Flow, Cell Biology, techniques