CST BLOG: Lab Expectations

The official blog of Cell Signaling Technology® (CST) where we discuss what to expect from your time at the bench, share tips, tricks, and information.

Which fluorophores can I combine in my flow cytometry experiment?

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Which fluorophores can I combine in my flow cytometry experiment?

Flow cytometry offers the exciting ability to detect and quantify multiple readouts per cell, enabli...
Rob M Apr 13, 2022 3:00:00 AM

Antibody Staining Guide for Flow Cytometry

Getting started with flow cytometry or beginning to branch out from working solely with live cells t...
Rob M Mar 23, 2022 3:00:00 AM

Tools for Classifying Cells and Identifying Protein Localization

When do I want to classify cells? Why do I care where a protein localizes? These are actually two di...
Andrea T Jan 26, 2022 3:00:00 AM

Immunology: What cells have a lymphoid lineage and how are they identified?

In humans, hematopoietic stem cells (HSCs) continuously replenish all types of blood cells via a ser...
Emma E Jan 19, 2022 3:00:00 AM

Why use viability dyes in flow cytometry experiments?

Viability dyes are widely used when counting and passaging cells, assessing chemical toxicity, and p...
Rob M Nov 17, 2021 3:00:00 AM

4 Situations Where Flow Cytometry Might Be More Useful Than Western Blotting

Western blotting is used to detect proteins and analyze their expression using antibodies, but it do...
Chris S Dec 4, 2019 3:00:00 AM

Need cell type and function? Use flow cytometry!

What is flow cytometry and how is it used? Flow cytometry enables you to save time and analyze many ...
Tamar A. May 8, 2019 3:15:00 AM

When should I use flow cytometry for signaling instead of western blot?

Flow cytometry enables quantitative analysis of protein expression, signaling states, and physical c...
Chris S Jan 9, 2019 3:15:00 AM

Mastering Phospho-Flow: Immune Cell Activation Webinar

To characterize activation of immune cells, measurement of intracellular signaling using phospho-spe...
Chris S Jul 11, 2018 3:15:00 AM

Intracellular Flow Cytometry in Action

Traditionally, flow cytometry has been used to identify distinct cell types within a heterogeneous p...
Liana G Aug 12, 2015 8:00:00 AM
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