Why High Concentration doesn't always mean good Antibody Performance.


Posted by Carolyn P on Dec 14, 2016 3:00:00 AM

If you buy primary antibodies from different places, you may have noticed that many companies list the size of their products in micrograms per ml (i.e., by weight). You may have also noticed that CST does not - CST antibodies are listed by performance (e.g., 10 western blots) instead.

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Topics: Antibody Performance, Primary Antibodies

The Cost of a Failed Western Blot


Posted by Claire S on Sep 14, 2016 3:00:00 AM


Thinking about lab expenses isn’t as enjoyable as investigating your favorite signaling pathway. However, because research money is hard to come by, it is something that should be considered when picking your reagents for western blotting. It makes sense to keep the quality of your primary antibody in mind, because the success of the entire experiment depends on the antibody being reliable, specific, and sensitive. If the antibody does not perform as expected your experiment may fail . . . and the cost of a failed western blot may be more than you think.

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Topics: Antibody Performance, Antibody Validation, Western Blot, techniques, Reproducibility

Roundtable Discussion on Antibody Validation


Posted by Carolyn P on Feb 17, 2016 3:00:00 AM

Antibody Validation Roundtable: Specificity + Sensitivity + Reproducibility = Validation 

Concerns about the use of incorrectly or insufficiently characterized antibodies in biomedical research have been raised by recent reports of nonreproducible research studies. Non-reproducible research studies have resulted in loss of time, resources, and scientific reputation, with publication retractions occurring in certain cases.

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Topics: Antibody Performance, Primary Antibodies, Antibody Validation, Webinars, Reproducibility

5 Steps to Publication-Worthy IF Images


Posted by Tara W on Sep 23, 2015 8:00:00 AM

A picture is worth a thousand words, or in the case of immunofluorescent imaging, a thousand proteins. The images used to illustrate a scientific experiment should convey as much information as the text itself. Here at CST, we pride ourselves in the quality of our antibodies and our rigorous validation process. When we approve our primary antibodies for IF, we like to showcase them using high quality images generated in-house. Beyond our recommended IF protocols (check it out here), here are some additional considerations to make when planning your IF staining. 

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Topics: Protocols, Antibody Performance, Antibody Validation, IF-IC

The Cost of a Failed Western Blot


Posted by Claire S on Aug 13, 2015 8:13:02 PM


Thinking about lab expenses isn’t as enjoyable as investigating your favorite signaling pathway. However, because research money is hard to come by, it is something that should be considered when picking your reagents for western blotting. It makes sense to keep the quality of your primary antibody in mind, because the success of the entire experiment depends on the antibody being reliable, specific, and sensitive. If the antibody does not perform as expected your experiment may fail . . . and the cost of a failed western blot may be more than you think.

READ MORE >

Topics: Antibody Performance, Antibody Validation, Western Blot, techniques

Why High Concentration doesn't always mean good Antibody Performance.


Posted by Carolyn P on Jul 29, 2015 5:00:00 AM

If you buy primary antibodies from different places, you may have noticed that many companies list the size of their products in micrograms per ml (i.e., by weight). You may have also noticed that CST does not - CST antibodies are listed by performance (e.g., 10 western blots) instead.

READ MORE >

Topics: Antibody Performance, Primary Antibodies, techniques

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