Biological research is about expanding knowledge, so it's not uncommon for scientists to become interested in studying proteins for which antibodies have not yet been developed. How then, can scientists pursue mechanistic studies of these novel or less-characterized proteins? Epitope tagging is a powerful tool for these protein studies and is used in myriad experimental applications. However, care must be taken not only in choice of epitope tag, but in selection and validation of antibodies.
Topics: Antibody Performance
Interested in studying senescence? Understanding when and why cell cycle arrest occurs is critical to many fields of research, including (but not limited to) studies of development, aging, and cancer. We all know the best tools produce the best results, so make sure you have all your bases covered with this list of the top 10 targets for your senescence research!
The production of new cells through cellular proliferation impacts the development, growth, and maintenance of all tissues in the body. This process must be tightly regulated, since uncontrolled cell division – as seen in various cancers – can lead to tumor formation and disrupt organ function. These broad implications for biological activities highlight the importance of understanding and accurately measuring cellular proliferation in a variety of contexts.
Topics: Cell Biology
The health of cells in culture is critical to the success of your experiments. Have you ever been excited about the experimental results of a knock-down, drug treatment, or culture condition, only to realize later that the effects are skewed due to the amount of cell death that occurred in your samples? Measuring and comparing cell viability in your assays is important, whether it’s the data you’re pursuing or an important control in your experiment.
Topics: Cell Biology
For decades, immunohistochemistry (IHC) has been a powerful technique for the investigation and visualization of cellular components in their native histological context. IHC has served as an important tool in medicine – enabling the diagnosis of complex pathological conditions – and in basic research to advance the understanding of key biological processes.
Chromatin immunoprecipitation sequencing (ChIP-seq) is a flexible and powerful technique used by researchers to elucidate how gene regulation is involved with different biological events and with the progression of various conditions like cancer and neurodegenerative diseases.
Careful planning and the fine tuning of experimental protocols are key to ensuring clear, interpretable scientific results. This is especially true for immunohistochemistry (IHC) studies, where each step in the often multiday process – from tissue preparation to stain development – can significantly impact the final outcome and analysis. Often, the simultaneous examination of multiple antigens is required to address specific scientific questions, which further complicates IHC protocol development. A general understating of the steps necessary to optimize IHC for multiple targets is essential to achieve reliable results. So, what are these steps?
What is flow cytometry and how is it used?
Flow cytometry enables you to save time and analyze many characteristics of your cells in one experiment, using classic principles of antibody detection.
Finally, the finish line is approaching! You have completed your specific aims, significance and innovation, and the bulk of your research strategy. You have sent those files for numerous rounds of pre-peer review by your trusted colleagues and mentors. Now it’s time to focus on some smaller, yet still very important details.
In the previous post, we described how to write an effective significance and innovation section, focused on defining the problem and providing a high-level overview of your proposed solution. In this post, we’ll outline the approach, wherein you’ll expand upon the solution and illustrate exactly how you plan to conduct the research.