CST BLOG: Lab Expectations

The official blog of Cell Signaling Technology® (CST) where we discuss what to expect from your time at the bench, share tips, tricks, and information.

Mastering Phospho-Flow: Immune Cell Activation Webinar

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Mastering Phospho-Flow: Immune Cell Activation Webinar

To characterize activation of immune cells, measurement of intracellular signaling using phospho-specific antibodies combined with immune phenotyping using surface markers is ideal. Owing to the unique capability of flow cytometry to measure events at a single-cell level, “Phospho Flow” allows for the identification of rare signaling events during processes such as co-stimulatory signaling and manipulation of checkpoint inhibition. However, combining extracellular and intracellular antibodies in a flow protocol can present technical challenges. 
 
 

 
Topics in this presentation include:
  • How small protocol changes can impact flow cytometry results;
  • Examples of flow protocol optimization using phospho-specific antibody panels for T cell activation and innate immunity; 
  • Guidance on experimental design for combining phenotypic markers with phospho-specific intracellular targets;
  • Discussion on the use of fix-and-perm kits.

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Chris Sumner
Chris Sumner
Chris Sumner was the Editor-in-Chief of Lab Expectations. When he's not reading/writing about curing disease, he's hiking in the woods, playing guitar, or searching for the world's best lobster roll.

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