Simplifying proteomics part 1 introduced mass spectrometry-based proteomics for profiling post-translational modifications (PTMs). Part 2 explained how you can go fishing for low abundance PTM peptides from a complex mixture using PTMScan.
If visualization is more your thing, it might be helpful to watch the PTMScan tutorial video to learn more about:
- How PTMScan employs PTM-sequence motif and PTM-specific antibodies to immunoenrich PTM-containing peptides before LC-MS/MS
- How you can use PTMScan services and kits from CST to study PTMs like phosphorylation, acetylation, ubiquitination, methylation, and succinylation
- The PTMScan service workflow from experimental design to data analysis