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Webinar - BioPlex: a protein interaction network created from thousands of protein immunopurifications

BioPlex: a protein interaction network created from thousands of protein immunopurifications

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Steven Gygi, Ph.D
Professor in the Department of Cell Biology, Harvard Medical School

Protein-protein interactions form a network whose structure drives cellular function and whose organization informs all biological inquiry. Using high-throughput affinity-purification mass spectrometry, we identify interacting partners for 2,594 human proteins in HEK293T cells. The resulting network (BioPlex) contains... 23,744 interactions, 86% unknown, among 7,668 proteins. BioPlex accurately depicts known complexes, attaining 80-100% coverage for most CORUM complexes. Network structure reveals 4 key features: 1) BioPlex subdivides into >300 communities, uniting proteins with shared function. 2) Interactions predict 2,968 associations among co-occurring Pfam domains. 3) Attributes including localization, biological process, and molecular function were determined for thousands of proteins - many uncharacterized. 4) BioPlex reveals interactions of biological or clinical significance. To demonstrate complementary studies inspired by BioPlex, we interrogated interactions of wild-type and mutant VAPB variants implicated in familial Amyotrophic Lateral Sclerosis. The network provides a framework for hypothesis generation and refinement as applied to protein function, mechanism, and activity.

Viewers will:

  • Learn how affinity purifications can be performed at scale and the associated caveats.
  • Understand how an interaction network can be used to predict a protein’s cellular properties

BioPlex: a protein interaction network created from thousands of protein immunopurifications

More Proteomics webinars can be viewed here. Also be sure to browse webinars on a variety topics available on-demand.

Claire S
Claire S
Claire is a Science Writer at CST

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